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公开(公告)号：US20060134633A1
公开(公告)日：2006-06-22
申请号：US10768729
申请日：2004-01-28
申请人： Yi-Ju Chen , John Leamon , Kenton Lohman , Michael Ronan , Jonathan Rothberg , Maithreyan Srinivasan , Michael Weiner
发明人： Yi-Ju Chen , John Leamon , Kenton Lohman , Michael Ronan , Jonathan Rothberg , Maithreyan Srinivasan , Michael Weiner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.
摘要翻译： 本发明涉及DNA测序方法。 更具体地，本发明涉及通过使用封闭和未封闭的测序引物对DNA的有义链和反义链进行测序的方法。 简而言之，这些方法包括将未封闭引物退火至第一条核酸的步骤; 将第二封闭引物退火至核酸的第二链; 用聚合酶沿着第一链延长核酸; 终止第一个测序引物; 解封第二底漆; 并沿着第二链延长核酸。
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公开(公告)号：US20050079510A1
公开(公告)日：2005-04-14
申请号：US10767899
申请日：2004-01-28
申请人： Jan Berka , Yi-Ju Chen , John Leamon , Steve Lefkowitz , Kenton Lohman , Vinod Makhijani , Jonathan Rothberg , Gary Sarkis , Maithreyan Srinivasan , Michael Weiner
发明人： Jan Berka , Yi-Ju Chen , John Leamon , Steve Lefkowitz , Kenton Lohman , Vinod Makhijani , Jonathan Rothberg , Gary Sarkis , Maithreyan Srinivasan , Michael Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US20050130173A1
公开(公告)日：2005-06-16
申请号：US10767779
申请日：2004-09-22
申请人： John Leamon , Kenton Lohman , Jonathan Rothberg , Michael Weiner
发明人： John Leamon , Kenton Lohman , Jonathan Rothberg , Michael Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译： 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。
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公开(公告)号：US20060040297A1
公开(公告)日：2006-02-23
申请号：US11195254
申请日：2005-08-01
申请人： John Leamon , Kenton Lohman , Jonathan Rothberg , Michael Weiner
发明人： John Leamon , Kenton Lohman , Jonathan Rothberg , Michael Weiner
IPC分类号： C12Q1/68
CPC分类号： B01L3/502761 , B01L3/5027 , B01L3/5085 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G06F19/22
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
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公开(公告)号：US20080003142A1
公开(公告)日：2008-01-03
申请号：US11803104
申请日：2007-05-11
申请人： Darren Link , Michael Weiner , David Marran , Jonathan Rothberg
发明人： Darren Link , Michael Weiner , David Marran , Jonathan Rothberg
CPC分类号： B01F3/0807 , B01F13/0062 , B01J19/0093 , B01L3/502715 , B01L3/502746 , B01L3/502784 , B01L3/565 , B01L7/525 , B01L9/527 , B01L2200/027 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/165 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/086 , B03C5/005 , B03C5/026 , C12N15/1068 , C12N15/1086 , C12Q1/00 , C12Q1/6806 , C12Q1/6837 , C12Q1/6844 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/628 , C12Q2565/629 , G01N15/147 , G01N21/05 , G01N21/64 , G01N27/3275 , G01N35/08 , G01N2021/0346 , G01N2035/00237 , G01N2035/00326 , G01N2201/024 , Y10T137/87571 , Y10T137/87619 , Y10T137/87652 , C12Q2525/191 , C12Q2563/159 , C12Q2535/101 , C12Q2563/149 , C12Q2531/113
摘要： The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
摘要翻译： 本发明提供了可用于进行生物学，化学和诊断测定的新型微流体底物和方法。 基板可以包括多个可电寻址的通道支承流体模块，其一体地布置成使得提供用于不混溶流体流动的连续通道。
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公开(公告)号：US20060160178A1
公开(公告)日：2006-07-20
申请号：US10991309
申请日：2004-11-17
申请人： Jonathan Rothberg , Michael Weiner
发明人： Jonathan Rothberg , Michael Weiner
CPC分类号： C07K16/2803 , C07K16/241 , C07K2317/34 , C07K2317/622
摘要： The present invention provides a high throughput method for generating fully human monoclonal antibodies.
摘要翻译： 本发明提供了用于产生完全人单克隆抗体的高通量方法。
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公开(公告)号：US20080014589A1
公开(公告)日：2008-01-17
申请号：US11803101
申请日：2007-05-11
申请人： Darren Link , Michael Weiner , David Marran , Jonathan Rothberg
发明人： Darren Link , Michael Weiner , David Marran , Jonathan Rothberg
IPC分类号： C12Q1/68 , B01D57/00 , B81B1/00 , C12N9/00 , G01N1/28 , G01N27/26 , G01N33/00 , G01N11/00 , C40B50/08 , C12P19/34 , C12Q1/00
CPC分类号： B01F3/0807 , B01F13/0062 , B01J19/0093 , B01L3/502715 , B01L3/502746 , B01L3/502784 , B01L3/565 , B01L7/525 , B01L9/527 , B01L2200/027 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/165 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/086 , B03C5/005 , B03C5/026 , C12N15/1068 , C12N15/1086 , C12Q1/00 , C12Q1/6806 , C12Q1/6837 , C12Q1/6844 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/628 , C12Q2565/629 , G01N15/147 , G01N21/05 , G01N21/64 , G01N27/3275 , G01N35/08 , G01N2021/0346 , G01N2035/00237 , G01N2035/00326 , G01N2201/024 , Y10T137/87571 , Y10T137/87619 , Y10T137/87652 , C12Q2525/191 , C12Q2563/159 , C12Q2535/101 , C12Q2563/149 , C12Q2531/113
摘要： The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
摘要翻译： 本发明提供了可用于进行生物学，化学和诊断测定的新型微流体底物和方法。 基板可以包括多个可电寻址的通道支承流体模块，其一体地布置成使得提供用于不混溶流体流动的连续通道。
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公开(公告)号：US20070092872A1
公开(公告)日：2007-04-26
申请号：US10222298
申请日：2002-08-15
申请人： Jonathan Rothberg , Joel Bader , Scott Dewell , Keith McDade , John Simpson , Jan Berka , Christopher Colangelo , Michael Weiner
发明人： Jonathan Rothberg , Joel Bader , Scott Dewell , Keith McDade , John Simpson , Jan Berka , Christopher Colangelo , Michael Weiner
CPC分类号： G01N21/6452 , B01L3/5027 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2300/0893 , B82Y15/00 , B82Y30/00 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , G01N21/0303 , G01N21/7703 , C12Q2565/519 , C12Q2533/101 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.
摘要翻译： 本文公开了用于测序核酸的方法和装置。 这些方法允许非常大量的独立测序反应并行排列，允许非常大数量（> 10,000）不同寡核苷酸的同时测序。
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9.发明申请Method for isolation of independent, parallel chemical micro-reactions using a porous filter 审中-公开使用多孔过滤器分离独立的，平行的化学微反应的方法公开(公告)号：US20050009022A1
公开(公告)日：2005-01-13
申请号：US10482491
申请日：2002-07-08
IPC分类号： C12N15/09 , B01D61/14 , B01D71/56 , B01J19/00 , C12M1/34 , C12M1/40 , C12Q1/68 , C12Q1/6869 , C40B40/06 , C40B40/10 , C40B60/14
CPC分类号： B01J19/0046 , B01J2219/00283 , B01J2219/00286 , B01J2219/00313 , B01J2219/00355 , B01J2219/00414 , B01J2219/00418 , B01J2219/00423 , B01J2219/00466 , B01J2219/00497 , B01J2219/005 , B01J2219/00524 , B01J2219/00576 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00641 , B01J2219/00648 , B01J2219/00659 , B01J2219/00677 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , C12Q1/6869 , C40B40/06 , C40B40/10 , C40B60/14
摘要： The present invention relates to the field of fluid dynamics. More specifically, this invention relates to methods and apparatus for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.
摘要翻译： 本发明涉及流体动力学领域。 更具体地，本发明涉及用于以基本上二维阵列并行进行密集包装的独立化学反应的方法和装置。 因此，本发明还着重于将该阵列用于诸如DNA测序，最优选焦磷酸测序和DNA扩增的应用。
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公开(公告)号：US20060163160A1
公开(公告)日：2006-07-27
申请号：US11042219
申请日：2005-01-25
申请人： Michael Weiner , John Lanzafame , John Hammond
发明人： Michael Weiner , John Lanzafame , John Hammond
IPC分类号： B01D61/00
CPC分类号： B01D63/08 , B01D61/20 , B01D63/06 , B01D63/088 , B01D2315/04
摘要： A processes for the separation and purification of microtubules of halloysite clay by separating an inlet stream of a liquid slurry of suspended clay particles flowing into a cross-flow filter into a decantate stream and a filtrate stream, the filtrate stream having a higher concentration of microtubular particles than the inlet stream. Also disclosed are subsequent preparations of novel structures and compositions of matter that include microtubules of halloysite clay.
摘要翻译： 通过将流入交叉流过滤器的悬浮粘土颗粒的液体浆料的入口流分离成倾析物流和滤液流来分离和纯化多层高岭土粘土的微管的方法，所述滤液流具有较高浓度的微管 颗粒比入口流。 还公开了随后的包括多水高岭土粘土的微管的物质结构和组成的制备。
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