公开(公告)号：US5529926A

公开(公告)日：1996-06-25

申请号：US423441

申请日：1995-04-19

申请人： Jan Maat ,  Wouter Musters ,  Hein Stam ,  Peter J. Schaap ,  Peter J. van de Vonderwoort ,  Jacob Visser ,  Johannes M. Verbakel

发明人： Jan Maat ,  Wouter Musters ,  Hein Stam ,  Peter J. Schaap ,  Peter J. van de Vonderwoort ,  Jacob Visser ,  Johannes M. Verbakel

IPC分类号： A21D8/04 ,  A23C9/12 ,  C07K14/00 ,  C07K14/37 ,  C07K14/41 ,  C12H1/00 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/09 ,  C12N15/53 ,  C12N15/80 ,  C12Q1/68 ,  C12R1/07 ,  C12R1/225 ,  C12R1/46 ,  C12R1/645 ,  C12R1/66 ,  C12R1/665 ,  C12R1/685 ,  C12R1/78 ,  C12R1/84 ,  C12R1/85 ,  C12N1/20

CPC分类号： C12Y108/03002 ,  A21D8/042 ,  A23C9/1213 ,  C12H1/003 ,  C12N15/80 ,  C12N9/0051

摘要： The invention relates to recombinant DNA technology for the production of an enzyme having sulfhydryl oxidase ("SOX") activity. This SOX-enzyme can be used where the oxidation of free sulfhydryl groups (thio compounds) to the corresponding disulfides is desirable. SOX enzyme may be used for treatment of bakery products or for removal of off-flavour from milk or beer.

摘要翻译： 本发明涉及用于生产具有巯基氧化酶（“SOX”）活性的酶的重组DNA技术。 这种SOX酶可用于将游离巯基（硫代化合物）氧化成相应二硫化物的地方。 SOX酶可用于处理面包店产品或从牛奶或啤酒中除去异味。

公开(公告)号：US5133893A

公开(公告)日：1992-07-28

申请号：US726639

申请日：1991-07-02

申请人： David Thom ,  Ton Swarthoff ,  Jan Maat

发明人： David Thom ,  Ton Swarthoff ,  Jan Maat

IPC分类号： C12N9/20 ,  C11D3/386 ,  C11D10/02 ,  C12R1/01 ,  C12R1/38

CPC分类号： C11D3/38627

摘要： The invention relates to a detergent composition comprising lipases. By inclusion of a certain immunologically defined class of lipases in a detergent composition which comprises as detergent-active material solely an anionic synthetic detergent, and as builder a water-soluble inorganic or organic builder salt, an improved overall detergency is obtained. The builder salt is typically sodium tripolyphosphate or sodium carbonate, and the lipase is typically obtained from certain Pseudomonas or Chromobacter strains.

摘要翻译： 本发明涉及包含脂肪酶的洗涤剂组合物。 通过在洗涤剂组合物中包含某些免疫学定义类别的脂肪酶，其仅包含作为洗涤剂活性物质的阴离子合成洗涤剂，并且作为助洗剂是水溶性无机或有机助洗剂盐，获得了改善的总洗涤性。 助洗剂盐通常是三聚磷酸钠或碳酸钠，并且脂肪酶通常从某些假单胞菌属或Chromobacter菌株获得。

公开(公告)号：US4966842A

公开(公告)日：1990-10-30

申请号：US194923

申请日：1988-05-17

申请人： Cornelis T. Verrips ,  Jan Maat ,  Luppo Edens ,  Adrianus M. Ledeboer

发明人： Cornelis T. Verrips ,  Jan Maat ,  Luppo Edens ,  Adrianus M. Ledeboer

IPC分类号： C12N15/09 ,  C07K14/43 ,  C12N1/20 ,  C12N1/21 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

CPC分类号： C07K14/43

摘要： The invention relates to structural genes consisting of DNA sequences encoding non-processed and partly processed thaumatin, to the various allelic forms of said non-processed thaumatin and to recombinant DNA's and plasmids comprising said structural genes coding for the various allelic forms of preprothaumatin, and naturally and/or artificially modified preprothaumatin in various stages of its natural processing, and to the use of said recombinant plasmids to transform microorganisms, particularly bacteria in which said genes are expressed.

公开(公告)号：US5741672A

公开(公告)日：1998-04-21

申请号：US473295

申请日：1995-06-07

申请人： Adrianus M. Ledeboer ,  Jan Maat ,  Cornelis T. Verrips ,  Christiaan Visser ,  Zbigniew A. Janowicz ,  Cornelis P. Hollenberg

发明人： Adrianus M. Ledeboer ,  Jan Maat ,  Cornelis T. Verrips ,  Christiaan Visser ,  Zbigniew A. Janowicz ,  Cornelis P. Hollenberg

IPC分类号： C11D3/386 ,  C12N9/04 ,  C12N9/10 ,  C12P21/00 ,  C07H21/04 ,  C12N1/19 ,  C12N15/00

CPC分类号： C12N9/0006 ,  C11D3/38654 ,  C12N9/1022 ,  A01K2217/05

摘要： The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, 0in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.

摘要翻译： 已经分离了多形汉逊酵母的醇氧化酶（MOX）和二羟基丙酮合成酶（DHAS）的结构基因及其调控DNA序列，确定了核苷酸序列。 本发明涉及MOX基因的用途，以及MOX和/或DAS的调节DNA序列与MOX基因组合使用，任选地在其修饰之后，或其它氧化酶基因或其他基因产生 工程微生物，在特定酵母中。 所述工程微生物可以以允许大规模工业应用的产率产生氧化酶或其它酶。 此外，所述工程微生物可以产生具有相对于其在氧化反应和/或漂白和洗涤剂产品中的应用具有改进性能的氧化酶。

公开(公告)号：US4891316A

公开(公告)日：1990-01-02

申请号：US742139

申请日：1985-06-07

申请人： Cornelis T. Verrips ,  Adrianus M. Ledeboer ,  Luppo Edens ,  Robert Klok ,  Jan Maat

发明人： Cornelis T. Verrips ,  Adrianus M. Ledeboer ,  Luppo Edens ,  Robert Klok ,  Jan Maat

IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/43 ,  C12N1/20 ,  C12N1/21 ,  C12N15/31 ,  C12N15/66 ,  C12N15/70 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

CPC分类号： C07K14/43 ,  C12N15/66 ,  C12N15/70

摘要： The invention relates to DNA sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles comprising said DNA sequences and their use in transforming microorganisms.

摘要翻译： 本发明涉及编码成熟的马蹄拟糖的各种等位基因形式的DNA序列，以及包含所述DNA序列的克隆载体及其在转化微生物中的用途。

公开(公告)号：US4707291A

公开(公告)日：1987-11-17

申请号：US870252

申请日：1986-06-03

申请人： David Thom ,  Ton Swarthoff ,  Jan Maat

发明人： David Thom ,  Ton Swarthoff ,  Jan Maat

IPC分类号： C11D10/02 ,  C11D3/386 ,  C12N9/20 ,  C12R1/01 ,  C12R1/38 ,  C11D7/42

CPC分类号： C11D3/38627

摘要： The invention relates to a detergent composition comprising lipases. By inclusion of a certain, immunologically defined class of lipases in a detergent composition which comprises a mixture of an anionic and a nonionic detergent, an improved overall detergency is obtained. Typical suitable lipases are obtained from certain Pseudomonas and Chromobacter strains.

摘要翻译： 本发明涉及包含脂肪酶的洗涤剂组合物。 通过在包含阴离子和非离子洗涤剂的混合物的洗涤剂组合物中包含某些免疫限定类型的脂肪酶，获得了改进的总洗涤性。 典型的合适的脂肪酶是从某些假单胞菌属和Chromobacter菌株获得的。

公开(公告)号：US06586209B1

公开(公告)日：2003-07-01

申请号：US07955726

申请日：1993-02-18

申请人： Robert F. M. van Gorcom ,  Johanna G. M. Hessing ,  Jan Maat ,  Martinus Roza ,  Johannes Maria A. Verbakel

发明人： Robert F. M. van Gorcom ,  Johanna G. M. Hessing ,  Jan Maat ,  Martinus Roza ,  Johannes Maria A. Verbakel

IPC分类号： C12N1509

CPC分类号： C12Y302/01032 ,  A21D8/042 ,  C12N9/248 ,  C12Y302/01008

摘要： An isolated DNA with a nucleotide sequence encoding a ripening form of a xylanase fungal origin having bread improving activity. Cells are transformed with this DNA and used to produce the ripening form of xylanase which itself is suitable for use in flour and dough.

摘要翻译： 具有编码具有面包改良活性的木聚糖酶真菌来源的成熟形式的核苷酸序列的分离的DNA。 细胞用该DNA转化并用于产生本身适用于面粉和面团的木聚糖酶的成熟形式。

公开(公告)号：US5683909A

公开(公告)日：1997-11-04

申请号：US135133

申请日：1993-10-12

申请人： Jan Kok ,  Jan Maat ,  Josephus Mauritius van der Vossen ,  Gerard Venema

发明人： Jan Kok ,  Jan Maat ,  Josephus Mauritius van der Vossen ,  Gerard Venema

IPC分类号： C12N15/74 ,  C12N15/75 ,  C12N15/69 ,  C12N15/70

CPC分类号： C12N15/746 ,  C12N15/75

摘要： The claimed invention is drawn to a recombinant plasmid which can replicate in Bacillus subtilis, Escherichia coli, and lactic acid Streptococcus bacteria comprising the replication of origin from Streptococcus cremoris plasmid pWV01 as its origin of replication, in addition to coding marker genes and genes of interest which code for improved fermenting properties.

摘要翻译： 所要求保护的发明涉及一种重组质粒，除了编码标记基因和感兴趣的基因之外，还可以在枯草芽孢杆菌，大肠杆菌和乳酸链球菌中复制，其包含起始于来自链球菌链球菌质粒pWV01的复制物作为其复制起点 该代码用于改善发酵性能。

公开(公告)号：US5240838A

公开(公告)日：1993-08-31

申请号：US587555

申请日：1990-09-24

申请人： Adrianus M. Ledeboer ,  Jan Maat ,  Cornelis T. Verrips ,  Christiaan Visser ,  Zbigniew A. Janowicz ,  Cornelis P. Hollenberg

发明人： Adrianus M. Ledeboer ,  Jan Maat ,  Cornelis T. Verrips ,  Christiaan Visser ,  Zbigniew A. Janowicz ,  Cornelis P. Hollenberg

IPC分类号： C07K14/60 ,  C11D3/386 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/68 ,  C12N15/81 ,  C12N15/90

CPC分类号： C12N9/0022 ,  C07K14/60 ,  C11D3/38654 ,  C12N15/68 ,  C12N15/81 ,  C12N15/815 ,  C12N15/90 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/1022 ,  A01K2217/05 ,  C07K2319/02 ,  C07K2319/75

摘要： The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.

摘要翻译： 已经分离了多形汉逊酵母的醇氧化酶（MOX）和二羟基丙酮合成酶（DHAS）的结构基因及其调控DNA序列，确定了核苷酸序列。 本发明涉及MOX基因的用途，以及MOX和/或DAS的调节DNA序列与MOX基因组合使用，任选地在其修饰之后，或其它氧化酶基因或其他基因产生 工程微生物，特别是酵母菌。 所述工程微生物可以以允许大规模工业应用的产率产生氧化酶或其它酶。 此外，所述工程微生物可以产生具有相对于其在氧化反应和/或漂白和洗涤剂产品中的应用具有改进性能的氧化酶。

公开(公告)号：US5108765A

公开(公告)日：1992-04-28

申请号：US498260

申请日：1990-03-23

申请人： Jan Maat ,  Martinus Roza

发明人： Jan Maat ,  Martinus Roza

IPC分类号： A21D2/18 ,  A21D8/04

CPC分类号： A21D8/042

摘要： A composition is disclosed which comprises cellulase, xylanase, peroxidase, and optionally an oxidase. The composition may be incorporated in flour as an additive to dough for bread or other baked dough products such as puff pastry. Flour compositions comprising a bread improver composition of cellulase, peroxidase and optionally an oxidase, and process for improving baked goods by using same are also shown.