公开(公告)号：US5955324A

公开(公告)日：1999-09-21

申请号：US914155

申请日：1997-08-19

申请人： Jian Qiang Fan ,  Yuan Chuan Lee ,  Kaoru Takegawa ,  Shojiro Iwahara ,  Akihiro Kondo ,  Ikunoshin Kato

发明人： Jian Qiang Fan ,  Yuan Chuan Lee ,  Kaoru Takegawa ,  Shojiro Iwahara ,  Akihiro Kondo ,  Ikunoshin Kato

IPC分类号： C12P19/14 ,  C08B37/08 ,  C12P19/26

CPC分类号： C12P19/14

摘要： To provide a process for producing a carbohydrate or a glycoconjugate without forming any hydrolytic product of the remodeled sugar chain.A process for producing a carbohydrate or a glycoconjugate by a remodeling reaction in the presence of a glycosidase which performs the remodeling reaction in an aqueous medium containing a water-soluble ketone and/or dioxane. Examples of the glycosidase include endoglycosidases such as Endo-A. Examples of the ketone include acetone.

摘要翻译： 提供生产碳水化合物或糖缀合物而不形成改性糖链的任何水解产物的方法。 在糖苷酶存在下通过重构反应制备碳水化合物或糖缀合物的方法，所述糖苷酶在含有水溶性酮和/或二恶烷的水性介质中进行重塑反应。 糖苷酶的实例包括内切糖苷酶如Endo-A。 酮的实例包括丙酮。

公开(公告)号：US06482806B1

公开(公告)日：2002-11-19

申请号：US09125397

申请日：1998-08-18

申请人： Nobuto Koyama ,  Hiroaki Sagawa ,  Eiji Kobayashi ,  Tatsuji Enoki ,  Hua-Kang Wu ,  Eiji Nishiyama ,  Suzu Deguchi ,  Katsushige Ikai ,  Hiromu Ohnogi ,  Motoko Ueda ,  Akihiro Kondo ,  Ikunoshin Kato

发明人： Nobuto Koyama ,  Hiroaki Sagawa ,  Eiji Kobayashi ,  Tatsuji Enoki ,  Hua-Kang Wu ,  Eiji Nishiyama ,  Suzu Deguchi ,  Katsushige Ikai ,  Hiromu Ohnogi ,  Motoko Ueda ,  Akihiro Kondo ,  Ikunoshin Kato

IPC分类号： A61K31727

CPC分类号： C07H7/033 ,  A61K8/60 ,  A61Q11/00 ,  Y10S977/928 ,  Y10S977/929 ,  Y10S977/93

摘要： A product obtained by heating at least one substance selected from the following (a), (b) and (c). (a) uronic acid or uronic acid derivative; (b) a saccharide compound containing uronic acid or a saccharide compound containing uronic acid derivative; and (c) a substance containing a saccharide compound containing uronic acid or a substance containing a saccharide compound containing uronic acid derivative; and food, beverage or a pharmaceutical agent which is characterized in containing the above-mentioned heat-treated product.

摘要翻译： 通过加热选自以下（a），（b）和（c）中的至少一种物质获得的产物：（a）糖醛酸或糖醛酸衍生物;（b）含有糖醛酸或含有糖醛酸的糖类化合物的糖类化合物 酸衍生物; 和（c）含有糖醛酸的糖类化合物或含有糖醛酸衍生物的糖类化合物的物质的物质;以及含有上述热处理物的食品，饮料或药剂。

公开(公告)号：US5472669A

公开(公告)日：1995-12-05

申请号：US19593

申请日：1993-02-19

申请人： Kojiro Miki ,  Takeshi Mori ,  Hiromi Ohkawa ,  Yumi Hosokawa ,  Akihiro Kondo ,  Ikunoshin Kato

发明人： Kojiro Miki ,  Takeshi Mori ,  Hiromi Ohkawa ,  Yumi Hosokawa ,  Akihiro Kondo ,  Ikunoshin Kato

IPC分类号： B67B7/18 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10

CPC分类号： B67B7/182 ,  G01N35/0099 ,  G01N2035/00495 ,  G01N2035/0405 ,  G01N2035/103 ,  G01N35/109 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

摘要： Pretreatment apparatus and process for analysis of sugar, the apparatus including a mechanical work hand which moves a vial containing a sample to various stations where reagents are added to the vial and where other pretreatment processes are performed on the sample. The pretreatment apparatus automatically controls the movement of the vial from one station to another and the various processes performed at each station, the processes including removal and storage of the vial's cap, addition of reagents into the vial, centrifugation and heating of the sample, and evaporation of excess reagents.

摘要翻译： 用于分析糖的预处理装置和方法，该装置包括机械手工，其将含有样品的小瓶移动到将试剂加入到小瓶中并在样品上进行其它预处理过程的各个站。 预处理装置自动控制小瓶从一个站到另一个站的移动，以及在每个站处执行的各种过程，包括小瓶盖的移除和储存，向小瓶中加入试剂，离心和加热样品，以及 蒸发过量试剂。

公开(公告)号：US4975533A

公开(公告)日：1990-12-04

申请号：US209723

申请日：1988-06-22

申请人： Akihiro Kondo ,  Ikunoshin Kato ,  Akira Obayashi ,  Sumihiro Hase

发明人： Akihiro Kondo ,  Ikunoshin Kato ,  Akira Obayashi ,  Sumihiro Hase

IPC分类号： G01N33/66 ,  C07H23/00 ,  G01N21/78 ,  G01N33/52 ,  G01N33/533 ,  G01N33/58

CPC分类号： C07H23/00 ,  G01N33/52 ,  G01N33/533

摘要： A method for the fluorescent labelling of a sugar or a sugar chain by labelling the reducing end of the sugar or sugar chain with a fluorescent substance, wherein the improvement comprises first forming a Schiff base by reacting the surgar or sugar chain with the fluorescent substance in the presence of an acid which is substantially free from water, and second, reducing said Schiff base.

摘要翻译： 一种通过用荧光物质标记糖或糖链的还原末端来对糖或糖链进行荧光标记的方法，其中所述改进包括首先通过将外科手术或糖链与荧光物质反应形成希夫碱 存在基本上不含水的酸，其次，还原所述席夫碱。

公开(公告)号：US20090221065A1

公开(公告)日：2009-09-03

申请号：US12281601

申请日：2007-03-02

申请人： Akihiro Kondo ,  Wenzhe Li ,  Takatoshi Nakagawa ,  Nobuto Koyama ,  Naoyuki Taniguchi ,  Ikunoshin Kato

发明人： Akihiro Kondo ,  Wenzhe Li ,  Takatoshi Nakagawa ,  Nobuto Koyama ,  Naoyuki Taniguchi ,  Ikunoshin Kato

IPC分类号： C12N15/63 ,  C07H21/02

CPC分类号： C12N15/1137 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/14 ,  C12Y204/01068

摘要： Disclosed is a means for reducing the expression amount of a FUT8 gene, reducing the expression amount of a FUT8 protein, and/or reducing the expression amount of a product produced by the action of FUT8.

摘要翻译： 公开了减少FUT8基因的表达量，降低FUT8蛋白的表达量和/或降低由FUT8的作用产生的产物的表达量的方法。

公开(公告)号：US20050202064A1

公开(公告)日：2005-09-15

申请号：US11114071

申请日：2005-04-26

申请人： Nobuto Koyama ,  Hiroaki Sagawa ,  Eiji Kobayashi ,  Tatsuji Enoki ,  Hua-Kang Wu ,  Eiji Nishiyama ,  Suzu Deguchi ,  Katsushige Ikai ,  Hiromu Ohnogi ,  Motoko Ueda ,  Akihiro Kondo ,  Ikunoshin Kato

发明人： Nobuto Koyama ,  Hiroaki Sagawa ,  Eiji Kobayashi ,  Tatsuji Enoki ,  Hua-Kang Wu ,  Eiji Nishiyama ,  Suzu Deguchi ,  Katsushige Ikai ,  Hiromu Ohnogi ,  Motoko Ueda ,  Akihiro Kondo ,  Ikunoshin Kato

IPC分类号： A23L3/3562 ,  A61K8/60 ,  A61K31/19 ,  A61K31/70 ,  A61K31/7012 ,  A61K31/715 ,  A61K31/727 ,  A61K31/728 ,  A61K31/732 ,  A61K31/734 ,  A61K31/737 ,  A61K35/00 ,  A61K35/12 ,  A61K35/74 ,  A61K36/00 ,  A61L2/16 ,  A61P35/00 ,  A61Q11/00 ,  C07H7/033 ,  C08B37/00

CPC分类号： C07H7/033 ,  A61K8/60 ,  A61Q11/00 ,  Y10S977/928 ,  Y10S977/929 ,  Y10S977/93

摘要： A product obtained by heating at least one substance selected from the following (a), (b) and (c). (a) uronic acid or uronic acid derivative; (b) a saccharide compound containing uronic acid or a saccharide compound containing uronic acid derivative; and (c) a substance containing a saccharide compound containing uronic acid or a substance containing a saccharide compound containing uronic acid derivative; and food, beverage or a pharmaceutical agent which is characterized in containing the above-mentioned heat-treated product.

摘要翻译： 通过加热至少一种选自以下（a），（b）和（c）的物质而得到的产物。 （a）糖醛酸或糖醛酸衍生物; （b）含有糖醛酸的糖类化合物或含有糖醛酸衍生物的糖类化合物; 和（c）含有糖醛酸的糖类化合物或含有糖醛酸衍生物的糖类化合物的物质的物质; 以及食品，饮料或药剂，其特征在于含有上述热处理产品。

公开(公告)号：US5500342A

公开(公告)日：1996-03-19

申请号：US288768

申请日：1994-08-11

申请人： Tsuyoshi Miyamura ,  Mutsumi Sano ,  Akihiro Kondo ,  Ikunoshin Kato

发明人： Tsuyoshi Miyamura ,  Mutsumi Sano ,  Akihiro Kondo ,  Ikunoshin Kato

IPC分类号： C12Q1/34 ,  C07H3/06 ,  C07H5/04 ,  C07H13/04 ,  C07H15/12 ,  C07H15/26 ,  G01N33/50 ,  G01N33/574 ,  C12Q1/00 ,  C07G3/00 ,  C07H19/00 ,  C12Q1/54

CPC分类号： C07H13/04 ,  C07H15/26 ,  C07H3/06 ,  G01N33/5005 ,  G01N33/57469 ,  Y10S435/81

摘要： A more exact, reliable, and simple method for determining the structure of a sugar chain, a kit to be used for this method, and novel oligosaccharides useful for the method as standard sugar chains are provided. The method for determining the structure of an N-acetyllactosamine type of sugar chain is characterized by determining the site of linkage and the mode of linkage of sugar residues at the non-reducing terminal side of a .beta.-N-acetylglucosamine residue linked to an .alpha.-mannose residue in M3 core by means of detecting the presence of the .beta.-N-acetylglucosamine residue after enzymatic or chemical treatment of the sugar chain. The kit for determining the structure of an N-acetyllactosamine type of sugar chain is characterized by containing at least one of the oligosaccharides represented by the chemical formula 1. The oligosaccharide is represented by the chemical formula 4.

摘要翻译： 提供了确定糖链结构的更准确，可靠和简单的方法，用于该方法的试剂盒，以及用作该标准糖链的方法的新型寡糖。 用于确定N-乙酰乳糖胺型糖链结构的方法的特征在于确定与α连接的β-N-乙酰氨基葡萄糖残基的非还原性末端侧的连接位点和连接方式 通过检测糖链的酶或化学处理后β-N-乙酰氨基葡萄糖残基的存在，在M3核心中的 - 甘露糖残基。 用于测定N-乙酰乳糖胺型糖链结构的试剂盒的特征在于含有至少一种由化学式1表示的寡糖。寡糖由化学式4表示。

公开(公告)号：US5089230A

公开(公告)日：1992-02-18

申请号：US491565

申请日：1990-03-09

申请人： Akihiro Kondo ,  Yoshiyuki Kato ,  Ikunoshin Kato ,  Hisao Tsuruta

发明人： Akihiro Kondo ,  Yoshiyuki Kato ,  Ikunoshin Kato ,  Hisao Tsuruta

IPC分类号： B01J19/00 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10

CPC分类号： G01N35/02 ,  B01J19/0046 ,  B01J2219/0059 ,  G01N2035/00396 ,  G01N2035/00495 ,  G01N2035/00534 ,  Y10T436/11 ,  Y10T436/111666

摘要： A reagent reaction apparatus for automatically performing a reaction by introducing various kinds of reagents to a sample solution. The reagent reaction apparatus includes a number of sample vials within a containing section and a plunger valve with fluid introducing port and a fluid discharge port located on top of the containing section. A mechanism sequentially injects into the sample vial a predetermined amount of reagents into a sample vial containing a measured amount of sample solutions. The sample vial is held in a rotary cone in order to apply centrifugal force. Then, the sample solution and reagents are subjected top a temperature adjusting bath which is preselected for the required reaction temperature. A mechanism transports the final product in the sample vial to a discharge station.

公开(公告)号：US08003781B2

公开(公告)日：2011-08-23

申请号：US12281601

申请日：2007-03-02

申请人： Akihiro Kondo ,  Wenzhe Li ,  Takatoshi Nakagawa ,  Nobuto Koyama ,  Naoyuki Taniguchi ,  Ikunoshin Kato

发明人： Akihiro Kondo ,  Wenzhe Li ,  Takatoshi Nakagawa ,  Nobuto Koyama ,  Naoyuki Taniguchi ,  Ikunoshin Kato

IPC分类号： C07H21/02 ,  C07H21/04

CPC分类号： C12N15/1137 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/14 ,  C12Y204/01068

摘要： Disclosed is a means for reducing the expression amount of a FUT8 gene, reducing the expression amount of a FUT8 protein, and/or reducing the expression amount of a product produced by the action of FUT8.

摘要翻译： 公开了减少FUT8基因的表达量，降低FUT8蛋白的表达量和/或降低由FUT8的作用产生的产物的表达量的方法。

公开(公告)号：US08835177B2

公开(公告)日：2014-09-16

申请号：US11917853

申请日：2006-06-12

申请人： Takahiro Marui ,  Tatsuji Enoki ,  Hiroaki Sagawa ,  Ikunoshin Kato

发明人： Takahiro Marui ,  Tatsuji Enoki ,  Hiroaki Sagawa ,  Ikunoshin Kato

IPC分类号： C12N15/87 ,  C07K14/78 ,  C12N15/86 ,  C12N15/867

CPC分类号： C12N15/86 ,  C07K14/78 ,  C12N2533/52 ,  C12N2740/13045 ,  C12N2810/857

摘要： A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.

摘要翻译： 一种将基因转移到脂肪细胞或祖细胞脂肪细胞中的方法，包括在具有逆转录病毒结合位点和靶标的物质存在的情况下用具有外来基因的逆转录病毒载体感染所述脂肪细胞或祖细胞的步骤 分子中的细胞结合位点或具有逆转录病毒结合位点的物质与具有靶细胞结合位点的物质的混合物，所述靶细胞结合位点具有能够结合VLA-5的区域和/或 可以结合VLA-4的区域。