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公开(公告)号：US09309541B2
公开(公告)日：2016-04-12
申请号：US14643788
申请日：2015-03-10
摘要： Strains of cyanobacteria that produce high levels of alpha ketoglutarate (AKG) and pyruvate are disclosed herein. Methods of culturing these cyanobacteria to produce AKG or pyruvate and recover AKG or pyruvate from the culture are also described herein. Nucleic acid sequences encoding polypeptides that function as ethylene-forming enzymes and their use in the production of ethylene are further disclosed herein. These nucleic acids may be expressed in hosts such as cyanobacteria, which in turn may be cultured to produce ethylene.
摘要翻译： 本文公开了产生高水平的α-酮戊二酸（AKG）和丙酮酸的蓝细菌菌株。 本文还描述了培养这些蓝细菌以产生AKG或丙酮酸并从培养物中回收AKG或丙酮酸的方法。 编码作为乙烯形成酶的多肽的核酸序列及其在乙烯生产中的用途在此进一步公开。 这些核酸可以在诸如蓝细菌的宿主中表达，其又可以培养以产生乙烯。
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公开(公告)号：US20060228774A1
公开(公告)日：2006-10-12
申请号：US10553097
申请日：2004-04-16
申请人： Paul King , Maria Ghirardi , Michael Seibert
发明人： Paul King , Maria Ghirardi , Michael Seibert
CPC分类号： C12N9/0067 , C12N9/001
摘要： The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hyrdogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H2 in a light catalyzed reaction having water as the reactant.
摘要翻译： 本发明提供了用于生产H 2 O 2的耐氧铁 - 氢化酶（[Fe] - 氢化酶）。 还公开了用于氧耐受[Fe] - 氢化酶的设计和工程中的方法，以及用耐氧[Fe] - 生成酶转化和培养合适的宿主细胞的方法。 最后，本发明提供了在具有水作为反应物的轻催化反应中，利用转化的，不对氧不敏感的宿主细胞批量生产H 2 O 2的方法。
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公开(公告)号：US07732174B2
公开(公告)日：2010-06-08
申请号：US10543866
申请日：2003-10-01
摘要： A method of using sequential chemostat culture vessels to provide continuous H2 production, in which photosynthetic O2 evolution and H2 photoproduction are separated physically into two separate bioreactors, comprising: a) growing a microorganism culture able to continuously generate H2 by photosynthetically producing cells at about the early-to-late log state in a first photobioreactor operating as a sulfur chemostat under aerobic and/or conditions; b) continuously feeding cells from the first photobioreactor to a second photobioreactor operating under anaerobic conditions and sulfur deprivation conditions resulting from constant uptake of sulfate in the first bioreactor and a low rate of culture flow between the first and second bioreactors, for induction of hydrogenase and H2 photoproduction to allow for continuous cultivation of the microorganism's cells in the first photobioreactor and constant H2 production in the second photobioreactor, and c) H2 gas from the second photobioreactor.
摘要翻译： 一种使用顺序恒化器培养容器提供连续H 2生产的方法，其中光合O 2进化和H 2光生产在物理上分离成两个单独的生物反应器，其包括：a）生长能够通过光合生产细胞在约 在有氧和/或条件下作为硫化学恒化器运行的第一个光生物反应器中的早期到晚期对数状态; b）将细胞从第一光生物反应器连续供给到在厌氧条件下操作的第二光生物反应器和由第一生物反应器中的硫酸盐持续摄取而产生的硫剥夺条件以及第一和第二生物反应器之间的较低培养流速，用于诱导氢化酶和 H2光生产以允许在第一光生物反应器中连续培养微生物细胞并在第二光生物反应器中恒定生成H2，以及c）来自第二光生物反应器的H2气。
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4.发明授权公开(公告)号：US07003101B2
公开(公告)日：2006-02-21
申请号：US10226048
申请日：2002-08-23
申请人： Michael Seibert
发明人： Michael Seibert
IPC分类号： H04M9/08
CPC分类号： H04B3/23
摘要： A method of controlling an echo canceller in a communications channel, is disclosed wherein input signals from the communications channel are first subbanded into a subband. Echo locations are then identified within the subband and are used to control the echo canceller. Typically, the echo canceller will be a fullband echo canceller with an adaptive filter, in which case the echo locations are used to control the filter coefficients.
摘要翻译： 公开了一种在通信信道中控制回波消除器的方法，其中来自通信信道的输入信号首先被分频成子带。 然后在子带内识别回波位置，并用于控制回波消除器。 通常，回波消除器将是具有自适应滤波器的全频带回波消除器，在这种情况下，回波位置用于控制滤波器系数。
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5.发明授权Double-talk and path change detection using a matrix of correlation coefficients 有权使用相关系数矩阵进行双向通话和路径变化检测公开(公告)号：US06944288B2
公开(公告)日：2005-09-13
申请号：US10042237
申请日：2002-01-11
申请人： Michael Seibert
发明人： Michael Seibert
CPC分类号： H04B3/234
摘要： A process is described which generates matrix coefficients using zero-lag auto and cross-correlations from signals commonly found in echo cancellers. Double-talk and path changes are then detected using matrix operations such as determinants, eigendecompositions, or singular value decompositions (SVDs). In a preferred embodiment, the determinant of the correlation-based matrix is compared against predetermined threshold values.
摘要翻译： 描述了使用来自回波消除器中通常发现的信号的零滞后自动和互相关产生矩阵系数的过程。 然后使用诸如决定因素，特征分解或奇异值分解（SVD）的矩阵运算来检测双向通话和路径改变。 在优选实施例中，将基于相关的矩阵的行列式与预定阈值进行比较。
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公开(公告)号：US08663958B2
公开(公告)日：2014-03-04
申请号：US12360756
申请日：2009-01-27
申请人： Paul King , Maria Lucia Ghirardi , Michael Seibert
发明人： Paul King , Maria Lucia Ghirardi , Michael Seibert
CPC分类号： C12N9/0067 , C12N9/001
摘要： The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H2 in a light catalyzed reaction having water as the reactant.
摘要翻译： 本发明提供用于生产H2的耐氧铁 - 氢化酶（[Fe] - 氢化酶）。 公开了用于氧耐受[Fe] - 氢化酶的设计和工程中的方法，以及用耐氧[Fe] - 氢化酶转化和培养合适的宿主细胞的方法。 最后，本发明提供了在具有水作为反应物的轻催化反应中，在大量生产H 2中利用转化的，不对氧不敏感的宿主细胞的方法。
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公开(公告)号：US20130176212A1
公开(公告)日：2013-07-11
申请号：US13345263
申请日：2012-01-06
IPC分类号： G06F3/033
CPC分类号： G06F3/033 , G06F3/0481 , G06F3/0482 , G06F3/0486 , G06F2203/04101 , G06F2203/04803
摘要： In graphical computing environments, applications are often presented as windows and other regions with visual controls that enable standardized region management functions, including repositioning the regions. However, in some scenarios (particularly devices with small displays), such user interface “chrome” may undesirable diminish the size of the region devoted to the output of the application, and removing such visual controls without eliminating repositioning functionality may be advantageous. Presented herein are repositioning gestures that may be invoked to request repositioning of a selected region by “hovering” a pointer near an edge of the display that is near the selected region; optionally by activating the pointer; and by moving the pointer away from the edge of the display (e.g., “dragging” the pointer while depressing a mouse button). This gesture may easily and unambiguously invoke a region repositioning mode for the selected region, despite the absence of visual repositioning controls confluent with the region.
摘要翻译： 在图形计算环境中，应用程序通常被视为具有可视化控件的窗口和其他区域，可实现标准化的区域管理功能，包括重新定位区域。 然而，在某些情况下（特别是具有小显示器的设备），这种用户界面“chrome”可能会不利地减小专用于应用的输出的区域的大小，并且在不消除重新定位功能的情况下去除这样的视觉控制可能是有利的。 这里呈现的是重新定位手势，其可以被调用以通过“靠近所选择的区域附近的显示器的边缘”悬停“指示重新定位所选择的区域; 可选地通过激活指针; 并且通过将指示器移动离开显示器的边缘（例如，在按下鼠标按钮的同时“拖动”指针）。 尽管没有与区域融合的视觉重新定位控制，这种手势可以容易且明确地调用所选区域的区域重新定位模式。
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公开(公告)号：US20070269874A1
公开(公告)日：2007-11-22
申请号：US10543866
申请日：2003-10-01
申请人： Sergey Kosourov , Maria Ghirardi , Michael Seibert
发明人： Sergey Kosourov , Maria Ghirardi , Michael Seibert
IPC分类号： C12P1/04
摘要： A method of using sequential chemostat culture vessels to provide continuous H2 production, in which photosynthetic O2 evolution and H2 photoproduction are separated physically into two separate bioreactors, comprising: a) growing a microorganism culture able to continuously generate H2 by photosynthetically producing cells at about the early-to-late log state in a first photobioreactor operating as a sulfur chemostat under aerobic and/or conditions; b) continuously feeding cells from the first photobioreactor to a second photobioreactor operating under anaerobic conditions and sulfur deprivation conditions resulting from constant uptake of sulfate in the first bioreactor and a low rate of culture flow between the first and second bioreactors, for induction of hydrogenase and H2 photoproduction to allow for continuous cultivation of the microorganism's cells in the first photobioreactor and constant H2 production in the second photobioreactor, and c) H2 gas from the second photobioreactor.
摘要翻译： 使用顺序恒化器培养容器提供连续H 2 O 2生产的方法，其中将光合作用的O 2进化和H 2光生产物理地分离成 两个单独的生物反应器，其包括：a）生长微生物培养物，其能够在有氧时在硫化学恒化器上操作的第一光生物反应器中以大约早期到晚期的对数状态光合生产细胞连续产生H 2 N 2 和/或条件; b）将细胞从第一光生物反应器连续供给到在厌氧条件下操作的第二光生物反应器和由第一生物反应器中的硫酸盐持续摄取而产生的硫剥夺条件以及第一和第二生物反应器之间的较低培养流速，用于诱导氢化酶和 H 2光生产以允许第一光生物反应器中的微生物细胞的连续培养和第二光生物反应器中的恒定H 2 N 2生产，以及c）H 2 / 来自第二光生物反应器的气体。
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9.发明授权Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms 失效使用含氢酶的含氧光合生物进行氢生产公开(公告)号：US06989252B2
公开(公告)日：2006-01-24
申请号：US09748690
申请日：2000-12-22
申请人： Anastasios Melis , Liping Zhang , John R. Benemann , Marc Forestier , Maria Ghirardi , Michael Seibert
发明人： Anastasios Melis , Liping Zhang , John R. Benemann , Marc Forestier , Maria Ghirardi , Michael Seibert
CPC分类号： C12N1/12 , C12P3/00 , Y10S435/946
摘要： A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.
摘要翻译： 可逆的生理过程提供了微生物中的氧释放和氢产生的时间分离，其包括在照明条件下在培养基中培养微生物培养物以累积内源底物的步骤，从培养基中消耗选自 由硫，铁和/或锰组成的组，将培养物与大气氧密封，将培养物轻轻孵育，由此光诱导的氧产生速率等于或小于呼吸速率，并收集放出的气体。 该方法特别有用于在微生物培养物中实现持续的光生物氢气生产，例如莱茵衣藻（Chlamydomonas reinhardtii）。
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公开(公告)号：US08890808B2
公开(公告)日：2014-11-18
申请号：US13345263
申请日：2012-01-06
IPC分类号： G06F3/033
CPC分类号： G06F3/033 , G06F3/0481 , G06F3/0482 , G06F3/0486 , G06F2203/04101 , G06F2203/04803
摘要： In graphical computing environments, applications are often presented as windows and other regions with visual controls that enable standardized region management functions, including repositioning the regions. However, in some scenarios (particularly devices with small displays), such user interface “chrome” may undesirable diminish the size of the region devoted to the output of the application, and removing such visual controls without eliminating repositioning functionality may be advantageous. Presented herein are repositioning gestures that may be invoked to request repositioning of a selected region by “hovering” a pointer near an edge of the display that is near the selected region; optionally by activating the pointer; and by moving the pointer away from the edge of the display (e.g., “dragging” the pointer while depressing a mouse button). This gesture may easily and unambiguously invoke a region repositioning mode for the selected region, despite the absence of visual repositioning controls confluent with the region.
摘要翻译： 在图形计算环境中，应用程序通常被视为具有可视化控件的窗口和其他区域，可实现标准化的区域管理功能，包括重新定位区域。 然而，在某些情况下（特别是具有小显示器的设备），这种用户界面“chrome”可能会不利地减小专用于应用的输出的区域的大小，并且在不消除重新定位功能的情况下去除这样的视觉控制可能是有利的。 这里呈现的是重新定位手势，其可以被调用以通过“靠近所选择的区域附近的显示器边缘附近的指针”悬停“来重新定位所选择的区域; 可选地通过激活指针; 并且通过将指示器移动离开显示器的边缘（例如，在按下鼠标按钮的同时“拖动”指针）。 尽管没有与区域融合的视觉重新定位控制，这种手势可以容易且明确地调用所选区域的区域重新定位模式。
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