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    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes
    1.
    发明申请
    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes 审中-公开
    用二苄唑胺染料标记多核苷酸的方法

    公开(公告)号：US20070099210A1

    公开(公告)日：2007-05-03

    申请号：US11466085

    申请日：2006-08-21

    申请人： Scott Benson ,  Joe Lam ,  Steven Menchen

    发明人： Scott Benson ,  Joe Lam ,  Steven Menchen

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C07D491/22

    CPC分类号： C09B11/24 ,  C07D209/60 ,  C07D221/10 ,  C07D311/78 ,  C07D491/14 ,  C07D491/147 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C09B11/02 ,  C12Q1/6869 ,  Y10T436/143333 ,  C12Q2563/107 ,  C12Q2527/125

    摘要： Dibenzorhodamine compounds having the structure are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.

    摘要翻译： 公开了具有该结构的二苄氧苯胺类化合物，包括其氮和芳基取代的形式。 此外，公开了可用于合成这些化合物的两种中间体，具有包括其氮和芳基取代形式的结构的第一中间体和具有其氮和芳基取代形式的结构的第二中间体，其中位置 C14至C18分别选自氢，氯，氟，低级烷基，羧酸，磺酸，-CH 2 OH，烷氧基，苯氧基，连接基团及其取代形式 。 本发明还包括包含二苄基罗丹胺化合物的能量转移染料，用二苯并呋喃胺化合物标记的核苷，以及使用二苯并呋喃胺化合物的核酸分析方法。

    UNNATURAL POLYMERASE SUBSTRATES THAT CAN SUSTAIN ENZYMATIC SYNTHESIS OF DOUBLE STRANDED NUCLEIC ACIDS FROM A NUCLEIC ACID TEMPLATE AND METHODS OF USE
    6.
    发明申请
    UNNATURAL POLYMERASE SUBSTRATES THAT CAN SUSTAIN ENZYMATIC SYNTHESIS OF DOUBLE STRANDED NUCLEIC ACIDS FROM A NUCLEIC ACID TEMPLATE AND METHODS OF USE 有权
    可以从核酸模板维持双重拉伸的核酸的酶合成和使用方法的UNNATURAL POLYMERASE底物

    公开(公告)号：US20090269759A1

    公开(公告)日：2009-10-29

    申请号：US12355487

    申请日：2009-01-16

    申请人： Steven M. Menchen, JR. ,  Barnett Rosenblum ,  Paul Kenney ,  Shoeb I. Khan ,  Zhaochun Ma ,  Jer-Kang Chen ,  Joe Lam ,  Boli Huang

    发明人： Steven M. Menchen, JR. ,  Barnett Rosenblum ,  Paul Kenney ,  Shoeb I. Khan ,  Zhaochun Ma ,  Jer-Kang Chen ,  Joe Lam ,  Boli Huang

    IPC分类号： C12Q1/68 ,  C07F9/02

    CPC分类号： C07H21/04 ,  C07H19/073 ,  C07H19/173 ,  C12Q1/6874 ,  C12Q2523/107 ,  C12Q2525/186 ,  C12Q2525/197

    摘要： Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3′ hydroxyl group. Methods of sequencing DNA using the substrates are also described.

    摘要翻译： 描述了可以维持来自核酸模板的双链核酸的酶合成的核苷酸类似物。 核苷酸类似物包括：（i）选自腺嘌呤，鸟嘌呤，胞嘧啶，胸腺嘧啶，尿嘧啶及其类似物的碱基; （ii）通过可切割连接体连接到碱基或类似物的标签; （iii）脱氧核糖; 和（iv）一个或多个磷酸酯基团。 接头和/或标记物将另外的核苷酸底物的模板定向聚合酶掺入到延伸的引物链上。 此外，接头的切割物留下连接到碱基上的残基，其不存在于天然核苷酸中，并且不抑制引物链的延伸。 因此，核苷酸类似物因此可以通过合成方法进行测序而不会阻断3'羟基作为可逆终止子。 还描述了使用底物测序DNA的方法。