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    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes
    1.
    发明申请
    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes 审中-公开
    用二苄唑胺染料标记多核苷酸的方法

    公开(公告)号：US20070099210A1

    公开(公告)日：2007-05-03

    申请号：US11466085

    申请日：2006-08-21

    申请人： Scott Benson ,  Joe Lam ,  Steven Menchen

    发明人： Scott Benson ,  Joe Lam ,  Steven Menchen

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C07D491/22

    CPC分类号： C09B11/24 ,  C07D209/60 ,  C07D221/10 ,  C07D311/78 ,  C07D491/14 ,  C07D491/147 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C09B11/02 ,  C12Q1/6869 ,  Y10T436/143333 ,  C12Q2563/107 ,  C12Q2527/125

    摘要： Dibenzorhodamine compounds having the structure are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.

    摘要翻译： 公开了具有该结构的二苄氧苯胺类化合物，包括其氮和芳基取代的形式。 此外，公开了可用于合成这些化合物的两种中间体，具有包括其氮和芳基取代形式的结构的第一中间体和具有其氮和芳基取代形式的结构的第二中间体，其中位置 C14至C18分别选自氢，氯，氟，低级烷基，羧酸，磺酸，-CH 2 OH，烷氧基，苯氧基，连接基团及其取代形式 。 本发明还包括包含二苄基罗丹胺化合物的能量转移染料，用二苯并呋喃胺化合物标记的核苷，以及使用二苯并呋喃胺化合物的核酸分析方法。

    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes
    6.
    发明申请
    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes 有权

    公开(公告)号：US20060063186A1

    公开(公告)日：2006-03-23

    申请号：US11226069

    申请日：2005-09-14

    申请人： Scott Benson ,  Steven Menchen ,  Krishna Upadhya

    发明人： Scott Benson ,  Steven Menchen ,  Krishna Upadhya

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/04

    CPC分类号： C07C245/08 ,  C07C50/18 ,  C07D311/90 ,  C07H21/00 ,  C07H21/04 ,  C09B6/00 ,  C09B11/08 ,  C09B33/02 ,  C09B35/021 ,  C09B35/50 ,  G01N33/542

    摘要： Dark quencher constructs, termed “multi-chromophoric quenchers” are described herein that comprise at least two dark quenching moieties, which can be the same or different, linked together by at least one multivalent linking moiety. The structure of the multi-chromophoric quenchers can be varied to selectively enhance quenching within a specific range of reporter emission wavelengths. This can be accomplished by linking together, into a single molecule, two or more identical quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to quench a broader range of reporter emission wavelengths than previously possible. This can be accomplished by linking together, into a single molecule, two or more different quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to simultaneously broaden the absorption range and increase the total absorption within the absorption range. This can be done by combining the two concepts described above. In other words, multiple types of quenching moieties can be employed to increase the absorption range and a multiple number of each type of quenching moiety can be used to increase the total absorptivity within the absorption range. The multi-chromophoric quenchers can be tethered to probes for biomolecules, insoluble supports and/or fluorescent dyes for use in a wide variety of biomolecular assays.

    Mobility-modifying cyanine dyes
    7.
    发明申请
    Mobility-modifying cyanine dyes 审中-公开
    迁移性改性花青染料

    公开(公告)号：US20050107617A1

    公开(公告)日：2005-05-19

    申请号：US10801092

    申请日：2004-03-15

    申请人： Steven Menchen ,  Scott Benson ,  Barnett Rosenblum ,  Shaheer Khan

    发明人： Steven Menchen ,  Scott Benson ,  Barnett Rosenblum ,  Shaheer Khan

    IPC分类号： C09B23/00 ,  C07F9/24 ,  C07H21/00 ,  C09B1/00 ,  C09B11/28 ,  C09B23/02 ,  G01N27/447 ,  G01N33/52 ,  G01N33/533 ,  C07D417/02 ,  C07D413/02 ,  C07D43/02

    CPC分类号： C07H21/00 ,  C07F9/2408 ,  C09B23/02 ,  G01N27/44726

    摘要： The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the hetercyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence-based 4-color nucleic acid sequencing reactions.

    摘要翻译： 本发明提供了一类新颖的荧光花青染料化合物，其在一个杂环氮原子上被修饰，具有迁移率调节部分，其允许调节或调谐用迁移率调节花青染料标记的多核苷酸的电泳迁移率 一种可预测的方式，同时保留酶活性。 可预测地调节染料的相对电泳迁移率的能力允许为各种应用（包括基于荧光的4色核酸测序反应）创建各种结构的迁移率匹配荧光染料组。

    Probe composition and method
    9.
    发明申请
    Probe composition and method 失效
    探头组成和方法

    公开(公告)号：US20050112609A1

    公开(公告)日：2005-05-26

    申请号：US10825624

    申请日：2004-04-14

    申请人： Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    发明人： Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q2600/156 ,  G01N27/447 ,  Y10T436/143333 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2537/143 ,  C12Q2565/131 ,  C12Q2565/125 ,  C12Q2565/107 ,  C12Q2521/501 ,  C12Q2563/155 ,  C12Q2563/119 ,  C12Q2549/119

    摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

    摘要翻译： 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。