-
公开(公告)号：USD518722S1
公开(公告)日：2006-04-11
申请号：US29210785
申请日：2004-08-04
-
公开(公告)号：USD536255S1
公开(公告)日：2007-02-06
申请号：US29210791
申请日：2004-08-04
-
公开(公告)号：USD533785S1
公开(公告)日：2006-12-19
申请号：US29210789
申请日：2004-08-04
-
公开(公告)号：US08597882B2
公开(公告)日：2013-12-03
申请号：US13365419
申请日：2012-02-03
申请人： John Corbett , John Corbett, Sr.
发明人： John Corbett , John Corbett, Sr.
CPC分类号： G01N35/0098 , B01L3/50851 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2300/0803 , B01L2300/1827 , B01L2400/0409 , G01N2035/00366 , G01N2035/0444
摘要： The present invention relates to methods and apparatus for conducting nucleic acid sequencing by pyrosequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support surface within each well, wherein the support surface is adapted to immobilize a first binding partner or selectively immobilize a second binding partner. The method further comprises the steps of binding or immobilizing the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well.
摘要翻译： 本发明涉及通过焦磷酸测序进行核酸测序的方法和装置。 该方法包括以下步骤：提供具有至少一个孔的平台，用于容纳至少一个支撑表面，并在每个孔内提供至少一个支撑表面，其中所述支撑表面适于固定第一结合伴侣或选择性地固定第二结合对 约束伙伴 该方法还包括以下步骤：将第一或第二结合伴侣结合或固定到支撑表面，并从所述平台外部的点将试剂分配到每个孔中，其中在分配步骤之后，平台被充分旋转，使得任何残留或 从每个孔和/或每个支撑表面基本上离心地去除未反应的所述试剂，其中在旋转期间每个支撑表面保持在每个孔内。
-
5.发明申请公开(公告)号：US20050233324A1
公开(公告)日：2005-10-20
申请号：US10512972
申请日：2003-05-01
申请人： John Corbett , John Corbett Jr
发明人： John Corbett , John Corbett Jr
CPC分类号： B01L7/52 , B01L7/5255 , B01L2200/147 , B01L2300/0627 , B01L2300/0803 , B01L2300/1844 , B01L2300/1866 , B04B15/02 , C12Q1/6816 , C12Q1/686 , G01N2035/00405 , G01N2035/0444 , C12Q2563/173 , C12Q2523/313
摘要： The invention relates to a device for the amplification of DNA in a reaction mixture, the device (1) comprising a heated chamber (2) including a rotor (3) for holding a plurality of reaction vessels for reaction mixtures, a drive means for the rotor, a microwave energy source (8) with means for controlled delivery of said energy to the reaction mixtures, and a system (14-16) for determining denaturation of double-stranded DNA. The invention also provides a method for the amplification of a nucleic acid strand. In the first step of the method, a reaction mixture is formed comprising the target nucleic acid strand, nucleotides, a primer, a thermostable nucleic acid polymerase, and, if necessary, a reagent for the detection of denaturation of double-stranded DNA. In the second step, the mixture is incubated at a temperature which allows synthesis of a nucleic acid strand complementary to the target nucleic acid strand. The third step comprises denaturing double-stranded DNA formed in the second step by microwave energisation of the reaction mixture with monitoring of the mixture to determine the denaturation end point. The reaction mixture is allowed to cool to a temperature at which primer anneals to the target nucleic acid strand in the fourth step. The second to fourth steps are repeated until a desired level of amplification is achieved.
摘要翻译： 本发明涉及用于在反应混合物中扩增DNA的装置，所述装置（1）包括加热室（2），该加热室包括用于保持多个用于反应混合物的反应容器的转子（3），驱动装置 转子，具有用于将所述能量控制递送到反应混合物的装置的微波能量源（8），以及用于确定双链DNA变性的系统（14-16）。 本发明还提供了用于扩增核酸链的方法。 在该方法的第一步中，形成包含靶核酸链，核苷酸，引物，热稳定性核酸聚合酶和必要时用于检测双链DNA变性的试剂的反应混合物。 在第二步中，将混合物在允许合成与靶核酸链互补的核酸链的温度下温育。 第三步包括在第二步中形成的双链DNA变性，通过微波激发反应混合物，监测混合物以确定变性终点。 将反应混合物冷却至第四步中靶核酸链的引物退火的温度。 重复第二至第四步骤，直到实现所需的放大水平。
-
公开(公告)号：US20060223061A1
公开(公告)日：2006-10-05
申请号：US10535666
申请日：2003-11-20
申请人： John Corbett , John Corbett
发明人： John Corbett , John Corbett
CPC分类号： G01N21/6428 , B01L3/502 , B01L3/50851 , B01L7/52 , B01L2300/0636 , B01L2300/0803 , B01L2300/1844 , B01L2300/1872 , B01L2400/0409 , G01N33/54366
摘要： The invention relates to a device for performing binding assays. In particular, the invention relates to a centrifugal device for performing such assays. The invention also relates to a method of performing binding assays involving antigen-antibody binding, nucleic acid hybridization, or receptor-ligand interaction.
摘要翻译： 本发明涉及用于进行结合测定的装置。 特别地，本发明涉及用于进行这种测定的离心装置。 本发明还涉及进行涉及抗原 - 抗体结合，核酸杂交或受体 - 配体相互作用的结合测定的方法。
-
公开(公告)号：US20060147912A1
公开(公告)日：2006-07-06
申请号：US10516186
申请日：2003-05-30
申请人： John Corbett , John Corbett
发明人： John Corbett , John Corbett
CPC分类号： B04B15/02 , B01L7/52 , B01L2200/147 , B01L2300/0803 , B01L2300/1844 , B01L2300/1872 , C12Q1/686 , C12Q2563/173
摘要： The invention relates to a method for the amplification of DNA and apparatus for such amplification. The method comprises steps typically found in amplification methods but utilises an optical procedure to detect denaturation of DNA or attainment of the desired denaturation temperature. The apparatus (1) comprises a temperature controllable chamber (2) including a rotor (3) for holding a plurality of reaction vessels (see 7 for example) for reaction mixtures including DNA, a drive means for the rotor, a heater (8) within the chamber for transiently supplying infrared energy to the reaction vessels, and an optical system (12-16) for determining denaturation of at least a reference DNA or for detecting attainment of a desired denaturation temperature in at least a reference reaction vessel.
摘要翻译： 本发明涉及扩增DNA的方法和用于扩增的装置。 该方法包括通常在扩增方法中发现的步骤，但利用光学方法检测DNA的变性或达到所需的变性温度。 装置（1）包括温度可控的室（2），其包括用于保持用于包括DNA的反应混合物的多个反应容器（例如7）的转子（3），用于转子的驱动装置，加热器（8） 在室内瞬时向反应容器提供红外能量;以及光学系统（12-16），用于确定至少参考DNA的变性或用于在至少参考反应容器中检测达到所需的变性温度。
-
公开(公告)号：US20130203049A1
公开(公告)日：2013-08-08
申请号：US13365419
申请日：2012-02-03
申请人： John Corbett , John Corbett, SR.
发明人： John Corbett , John Corbett, SR.
IPC分类号： G01N33/53
CPC分类号： G01N35/0098 , B01L3/50851 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2300/0803 , B01L2300/1827 , B01L2400/0409 , G01N2035/00366 , G01N2035/0444
摘要： The present invention relates to methods and apparatus for conducting nucleic acid sequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support surface within each well, wherein the support surface is adapted to immobilise a first binding partner or selectively immobilise a second binding partner. The method further comprises the steps of binding or immobilising the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well. The invention also relates to kits and uses of the kits for conducting nucleic acid sequencing. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.
摘要翻译： 本发明涉及用于进行核酸测序的方法和装置。 该方法包括以下步骤：提供具有至少一个孔的平台，用于容纳至少一个支撑表面，并在每个孔内提供至少一个支撑表面，其中所述支撑表面适于固定第一结合伴侣或选择性地固定第二结合对 约束伙伴 该方法还包括以下步骤：将第一或第二结合配偶体固定或固定到支撑表面，并从所述平台外部的点向试剂分配，其中在分配步骤之后，平台被充分旋转，使得任何残留或 从每个孔和/或每个支撑表面基本上离心地去除未反应的所述试剂，其中在旋转期间每个支撑表面保持在每个孔内。 本发明还涉及用于进行核酸测序的试剂盒和试剂盒的用途。 特别地，本发明主要用于通过焦磷酸测序对核酸进行测序，然而本发明不限于该领域。
-
公开(公告)号：US20120282708A1
公开(公告)日：2012-11-08
申请号：US13386834
申请日：2010-07-29
申请人： John Corbett , John Corbett, SR.
发明人： John Corbett , John Corbett, SR.
IPC分类号： G01N33/53
CPC分类号： G01N35/00029 , B01J2219/00326 , B01J2219/0049 , B01J2219/00536 , B01J2219/0061 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00722 , B01L3/50851 , B01L2300/0803 , B01L2300/0864 , B01L2400/0409 , G01N33/5308 , G01N33/582 , G01N35/00069 , G01N2035/00495
摘要： The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.
摘要翻译： 本发明涉及进行测定的方法和装置。 特别地，本发明涉及可用于进行测定，特别是多步测定的可旋转平台。 本发明提供了一种可旋转平台，其适于将第一结合伴侣固定在所述平台的表面上的一个或多个离散区域中，或者选择性地将第二结合伴侣固定在所述平台的表面上的一个或多个离散区域中。 本发明还涉及方法，装置，试剂盒以及用于进行测定的可旋转平台的使用。 特别地，本发明主要用于通过焦磷酸测序对核酸进行测序，然而本发明不限于该领域。
-
公开(公告)号：US08921040B2
公开(公告)日：2014-12-30
申请号：US13386834
申请日：2010-07-29
申请人： John Corbett , John Corbett, Sr.
发明人： John Corbett , John Corbett, Sr.
CPC分类号： G01N35/00029 , B01J2219/00326 , B01J2219/0049 , B01J2219/00536 , B01J2219/0061 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00722 , B01L3/50851 , B01L2300/0803 , B01L2300/0864 , B01L2400/0409 , G01N33/5308 , G01N33/582 , G01N35/00069 , G01N2035/00495
摘要： The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilize a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilize a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.
摘要翻译： 本发明涉及进行测定的方法和装置。 特别地，本发明涉及可用于进行测定，特别是多步测定的可旋转平台。 本发明提供了一种可旋转平台，其适于将第一结合伴侣固定在所述平台的表面上的一个或多个离散区域中，或者选择性地将第二结合伴侣固定在所述平台的表面上的一个或多个离散区域中。 本发明还涉及方法，装置，试剂盒以及用于进行测定的可旋转平台的使用。 特别地，本发明主要用于通过焦磷酸测序对核酸进行测序，然而本发明不限于该领域。
-
-
-
-
-
-
-
-
-
搜索结果
20 条记录 (耗时 0.036 秒)
