公开(公告)号：US4469631A

公开(公告)日：1984-09-04

申请号：US387009

申请日：1982-06-10

申请人： John D. Baxter ,  Ivy Fettes ,  John Shine

发明人： John D. Baxter ,  Ivy Fettes ,  John Shine

IPC分类号： C12N15/00 ,  C12P21/02 ,  C07C103/52 ,  C12P21/00

CPC分类号： C12P21/02 ,  C12N15/00 ,  C12R1/19 ,  Y10S530/808

摘要： DNA comprising the naturally occurring nucleotide sequence coding for amino acids 44-90 of .beta.-lipotropin and including the entire coding region for .beta.-endorphin with the exception of the C-terminal glutamine was modified, transferred to an expression transfer vector, and expressed as a fusion protein. The fusion protein was further modified in vitro to yield mature .beta.-endorphin. .beta.-endorphin was purified from a bacterial lysate. The structure and biological activity of the resulting product was proven by immunological assay, and by two independent assays designed to demonstrate biological activity.

摘要翻译： 包含编码β-Lipotropin的氨基酸44-90的天然存在的核苷酸序列的DNA被修饰，转移到表达转移载体，并表达为 融合蛋白。 融合蛋白在体外进一步修饰，产生成熟的β-内啡肽。 从细菌裂解物纯化β-内啡肽。 通过免疫测定证实所得产物的结构和生物活性，并通过设计用于证明生物活性的两种独立测定来证明。

公开(公告)号：US4350764A

公开(公告)日：1982-09-21

申请号：US128711

申请日：1980-03-10

申请人： John D. Baxter ,  Ivy Fettes ,  John Shine

发明人： John D. Baxter ,  Ivy Fettes ,  John Shine

IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/005 ,  C07K14/195 ,  C07K14/575 ,  C07K14/655 ,  C07K14/675 ,  C12N1/20 ,  C12P21/00 ,  C12P21/02 ,  C12N1/00 ,  C12N15/00

CPC分类号： C07K14/675 ,  C12R1/19 ,  Y10S435/82 ,  Y10S435/848 ,  Y10S435/849

摘要： DNA comprising the naturally occurring nucleotide sequence coding for amino acids 44-90 of .beta.-lipotropin and including the entire coding region for .beta.-endorphin with the exception of the C-terminal glutamine was modified, transferred to an expression transfer vector, and expressed as a fusion protein. The fusion protein was further modified in vitro to yield mature .beta.-endorphin. .beta.-endorphin was purified from a bacterial lysate. The structure and biological activity of the resulting product was proven by immunological assay, and by two independent assays designed to demonstrate biological activity.

摘要翻译： 包含编码β-Lipotropin的氨基酸44-90的天然存在的核苷酸序列的DNA被修饰，转移到表达转移载体，并表达为 融合蛋白。 融合蛋白在体外进一步修饰，产生成熟的β-内啡肽。 从细菌裂解物纯化β-内啡肽。 通过免疫测定证实所得产物的结构和生物活性，并通过设计用于证明生物活性的两种独立测定来证明。

公开(公告)号：US4322499A

公开(公告)日：1982-03-30

申请号：US972430

申请日：1978-12-22

申请人： John D. Baxter ,  James L. Roberts ,  Peter H. Seeburg ,  Howard M. Goodman ,  John Shine

发明人： John D. Baxter ,  James L. Roberts ,  Peter H. Seeburg ,  Howard M. Goodman ,  John Shine

IPC分类号： C07K14/665 ,  C12N1/00

CPC分类号： C07K14/665

摘要： A technique suitable for cloning a cDNA having a base sequence coding for the ACTH/LPH precursor is disclosed. The invention is exemplified by the cloning of a cDNA fragment comprising a base sequence coding for the endorphin region. The fragment, hereinafter termed the endorphin gene cDNA sequence, was obtained from cultured mouse pituitary tumor cells known to produce the ACTH/LPH precursor protein.

摘要翻译： 公开了一种适用于克隆编码ACTH / LPH前体碱基序列的cDNA的技术。 通过克隆包含编码内啡肽区域的碱基序列的cDNA片段来举例说明本发明。 该片段（以下称为内啡肽基因cDNA序列）从已知产生ACTH / LPH前体蛋白的培养的小鼠垂体肿瘤细胞获得。

公开(公告)号：US07122579B2

公开(公告)日：2006-10-17

申请号：US10374681

申请日：2003-02-25

申请人： Daniel M. Schwartz ,  John D. Baxter ,  Michele D. Jumper ,  Thomas S. Scanlan

发明人： Daniel M. Schwartz ,  John D. Baxter ,  Michele D. Jumper ,  Thomas S. Scanlan

IPC分类号： A61K31/195

CPC分类号： A61K31/195 ,  A61K45/06 ,  Y10S514/913

摘要： The present invention involves the discovery that synthetic thyroid hormones can be used as compositions to reduce intraocular pressure in vivo. Methods of screening synthetic thyroid hormones for effect on intraocular pressure, aqueous pressure, hydraulic conductivity, hyaluronic acid secretion, and extracellular matrix assembly are provided. Methods of treating glaucoma and treating excess intraocular pressure with synthetic thyroid hormones and compositions therefore are also provided.

公开(公告)号：US07302347B2

公开(公告)日：2007-11-27

申请号：US10317034

申请日：2002-12-10

申请人： John D. Baxter ,  Thomas S. Scanlan ,  Robert J. Fetterick ,  Sabine Borngraeber ,  Paul Webb

发明人： John D. Baxter ,  Thomas S. Scanlan ,  Robert J. Fetterick ,  Sabine Borngraeber ,  Paul Webb

IPC分类号： G01N33/53 ,  G06G7/48

CPC分类号： C40B40/04 ,  G01N33/743 ,  G01N2333/723 ,  G01N2800/108 ,  Y10S707/99943

摘要： This invention pertains to agonists that activate nuclear receptors. These agonists include an extension that contacts a region of the nuclear receptor outside the native ligand binding pocket. Methods for producing, identifying and designing such agonists are included along with nuclear receptor agonist complexes and libraries of agonists.

摘要翻译： 本发明涉及激活核受体的激动剂。 这些激动剂包括与天然配体结合口外的核受体的区域接触的延伸。 用于制备，鉴定和设计这种激动剂的方法包括核受体激动剂复合物和激动剂文库。

公开(公告)号：US06236946B1

公开(公告)日：2001-05-22

申请号：US08764870

申请日：1996-12-13

申请人： Thomas S. Scanlan ,  John D. Baxter ,  Robert J. Fletterick ,  Richard L. Wagner ,  Peter J. Kushner ,  James Apriletti ,  Brian West ,  Andrew K. Shiau

发明人： Thomas S. Scanlan ,  John D. Baxter ,  Robert J. Fletterick ,  Richard L. Wagner ,  Peter J. Kushner ,  James Apriletti ,  Brian West ,  Andrew K. Shiau

IPC分类号： G06F1900

CPC分类号： C07K14/72 ,  C07K2299/00

摘要： The present invention provides new methods, particularly computational methods, and compositions for the generation of nuclear receptor synthetic ligands based on the three dimensional structure of nuclear receptors, particularly the thyroid receptor (herein referred to as “TR”). Also provided are crystals, nuclear receptor synthetic ligands, and related methods.

摘要翻译： 本发明提供了基于核受体，特别是甲状腺受体（本文中称为“TR”）的三维结构产生核受体合成配体的新方法，特别是计算方法和组合物。 还提供了晶体，核受体合成配体和相关方法。

公开(公告)号：US6054485A

公开(公告)日：2000-04-25

申请号：US915232

申请日：1997-08-20

申请人： Daniel M. Schwartz ,  John D. Baxter ,  Michele D. Jumper ,  Thomas S. Scanlan

发明人： Daniel M. Schwartz ,  John D. Baxter ,  Michele D. Jumper ,  Thomas S. Scanlan

IPC分类号： A61K9/00 ,  A61K9/127 ,  A61K31/195 ,  A61K31/198 ,  A61K31/205 ,  A61K31/27 ,  A61K38/23 ,  A61K45/06 ,  A61K47/30 ,  A61P27/06 ,  A61P43/00 ,  G01N33/15 ,  G01N33/50 ,  G01N33/566

CPC分类号： A61K31/195 ,  A61K45/06 ,  Y10S514/913

摘要： The present invention involves the discovery that synthetic thyroid hormones can be used as compositions to reduce intraocular pressure in vivo. Methods of screening synthetic thyroid hormones for effect on intraocular pressure, aqueous pressure, hydraulic conductivity, hyaluronic acid secretion, and extracellular matrix assembly are provided. Methods of treating glaucoma and treating excess intraocular pressure with synthetic thyroid hormones and compositions therefore are also provided.

公开(公告)号：US06266622B1

公开(公告)日：2001-07-24

申请号：US08980115

申请日：1997-11-26

申请人： Thomas S. Scanlan ,  John D. Baxter ,  Robert J. Fletterick ,  Richard L. Wagner ,  Peter J. Kushner ,  James J. Apriletti ,  Brian L. West ,  Andrew K. Shiau

发明人： Thomas S. Scanlan ,  John D. Baxter ,  Robert J. Fletterick ,  Richard L. Wagner ,  Peter J. Kushner ,  James J. Apriletti ,  Brian L. West ,  Andrew K. Shiau

IPC分类号： G06F1900

CPC分类号： C07K14/723 ,  A61K31/192 ,  A61K31/195 ,  A61K31/198 ,  A61K31/27 ,  C07K14/72 ,  C07K2299/00 ,  G01N33/68 ,  G01N33/78 ,  G01N2333/723 ,  G01N2500/04 ,  G06F19/16

摘要： The present invention provides new methods, particularly computational methods, and compositions for the generation of nuclear receptor synthetic ligands based on the three dimensional structure of nuclear receptors, particularly the thyroid receptor (herein referred to as “TR”). Also provided are crystals, nuclear receptor synthetic ligands, and related methods.

公开(公告)号：US4675297A

公开(公告)日：1987-06-23

申请号：US532107

申请日：1983-09-14

申请人： John D. Baxter ,  Walter L. Miller ,  Joseph A. Martial

发明人： John D. Baxter ,  Walter L. Miller ,  Joseph A. Martial

IPC分类号： C07K14/575 ,  C12N1/20 ,  C07H21/04 ,  C12N1/00 ,  C12N15/00 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02

CPC分类号： C07K14/57554 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/75

摘要： A DNA sequence encoding bovine prolactin and optionally including condons for the preceding 10 amino acids is used to construct expression systems to obtain recombinant production of these proteins.

摘要翻译： 使用编码牛催乳素并任选地包含前10个氨基酸的宽带的DNA序列构建表达系统以获得这些蛋白质的重组产生。

公开(公告)号：US06692941B1

公开(公告)日：2004-02-17

申请号：US07480745

申请日：1990-02-15

申请人： Walter L. Miller ,  Joseph Augustin Martial ,  John D. Baxter

发明人： Walter L. Miller ,  Joseph Augustin Martial ,  John D. Baxter

IPC分类号： C12N1500

CPC分类号： C12N15/71 ,  C07K14/61 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/75

摘要： A DNA comprising a deoxynucleotide sequence coding for bovine growth hormone is described. A transfer vector and an expression vector containing this DNA and microorganisms transformed by these vectors are also described.

摘要翻译： 描述了包含编码牛生长激素的脱氧核苷酸序列的DNA。 还描述了转移载体和含有该DNA的表达载体和由这些载体转化的微生物。