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22 条记录 (耗时 0.023 秒)

    Method of operating ion trap detector in MS/MS mode
    3.
    发明授权
    Method of operating ion trap detector in MS/MS mode 失效
    在MS / MS模式下操作离子阱检测器的方法

    公开(公告)号：US4736101A

    公开(公告)日：1988-04-05

    申请号：US84518

    申请日：1987-08-11

    申请人： John E. P. Syka ,  John N. Louris ,  Paul E. Kelley ,  George C. Stafford ,  Walter E. Reynolds

    发明人： John E. P. Syka ,  John N. Louris ,  Paul E. Kelley ,  George C. Stafford ,  Walter E. Reynolds

    IPC分类号： G01N27/62 ,  G21K1/087 ,  H01J49/42

    CPC分类号： H01J49/424 ,  H01J49/0063 ,  H01J49/0081 ,  H01J49/429

    摘要： A simple and economical method of mass analyzing a sample by means of a quadrupole ion trap mass spectrometer in an MS/MS mode comprises the steps of forming ions within a trap structure, changing the RF and DC voltages in such a way that the ions with mass-to-charge ratios within a desired range will be and remain trapped within the trap structure, dissociating such ions into fragments by collisions and increasing the field intensity again so that the generated fragments will become unstable and exit the trap volume sequentially to be detected. A supplementary AC field may be applied additionally to provide various scan modes as well as dissociate the ions.

    摘要翻译： 以MS / MS模式通过四极离子阱质谱仪对样品进行质量分析的简单而经济的方法包括以下步骤：在捕获结构内形成离子，改变RF和DC电压，使得离子与 在所需范围内的质荷比将被捕获在陷阱结构内，并且通过碰撞将这些离子解离成碎片，并再次增加场强，使得所产生的片段将变得不稳定并顺序地离开陷阱体积以被检测 。 附加的AC场可以另外施加以提供各种扫描模式以及离子离解。

    Method of operating an ion trap mass spectrometer in a high resolution mode
    4.
    发明授权
    Method of operating an ion trap mass spectrometer in a high resolution mode 失效
    在高分辨率模式下操作离子质谱仪的方法

    公开(公告)号：US5182451A

    公开(公告)日：1993-01-26

    申请号：US849970

    申请日：1992-03-12

    申请人： Jae C. Schwartz ,  John E. P. Syka ,  John N. Louris

    发明人： Jae C. Schwartz ,  John E. P. Syka ,  John N. Louris

    IPC分类号： G01N27/62 ,  H01J49/34 ,  H01J49/42

    CPC分类号： H01J49/424 ,  H01J49/429

    摘要： A method of mass analyzing a sample including the steps of defining a trap volume with a three-dimensional quadrupole field for trapping ions within a predetermined range of mass-to-charge ratio, forming or injecting ions within the trap volume such that those within the predetermined mass-to-charge ratio range are trapped within the trap volume, applying a supplementary AC field superimposed on the three-dimensional quadrupole field to form combined fields, scanning the combined fields to eject ions of consecutive mass-to-charge ratio from the trap volume for detection characterized in that the supplementary field has an amplitude just sufficient to eject the ions and that the supplementary field has a beta value below 0.891 and that the combined fields are scanned at a rate so that a length of time corresponding to 200 cycles or more of the supplementary AC field passes per consecutive thomson.

    Reagents for electron transfer dissociation in mass spectrometry analysis
    8.
    发明授权
    Reagents for electron transfer dissociation in mass spectrometry analysis 有权
    质谱分析中电子转移离解试剂

    公开(公告)号：US09171707B2

    公开(公告)日：2015-10-27

    申请号：US13391331

    申请日：2010-09-02

    申请人： John E. P. Syka ,  Philip D. Compton ,  Donald F. Hunt

    发明人： John E. P. Syka ,  Philip D. Compton ,  Donald F. Hunt

    IPC分类号： G01N24/00 ,  H01J49/00

    CPC分类号： H01J49/0072 ,  Y10T436/24

    摘要： The invention provides improvements in reagents for use in electron transfer dissociation ionization techniques for use in mass spectrometry, particularly for sequencing peptides and proteins using mass spectrometric techniques involving electrospray ionization and MS/MS characterization of fragment ions. The novel reagents used in the inventive methods allow for more effective determination of protein sequences, especially of long peptides or post-translationally modified protein fragments. Use of the polycyclic aromatic hydrocarbons azulene, homoazulene, and acenaphthylene, and homodimers and heterodimers thereof, are described.

    摘要翻译： 本发明提供了用于质谱法中使用的电子转移离解电离技术的试剂的改进，特别是使用涉及电喷雾离子化和片段离子的MS / MS表征的质谱技术测序肽和蛋白质。 用于本发明方法的新颖试剂允许更有效地测定蛋白质序列，特别是长肽或翻译后修饰的蛋白质片段。 描述了多环芳香烃薁，高ene烯和苊烯及其同二聚体和异二聚体的用途。

    Ion source assembly for an ion trap mass spectrometer and method
    9.
    发明授权
    Ion source assembly for an ion trap mass spectrometer and method 失效
    离子源组件用于离子阱质谱仪和方法

    公开(公告)号：US5756996A

    公开(公告)日：1998-05-26

    申请号：US675966

    申请日：1996-07-05

    申请人： Mark E. Bier ,  John E. P. Syka ,  Dennis M. Taylor ,  William J. Fies

    发明人： Mark E. Bier ,  John E. P. Syka ,  Dennis M. Taylor ,  William J. Fies

    IPC分类号： H01J49/04 ,  H01J49/14 ,  H01J49/42

    CPC分类号： H01J49/147 ,  H01J49/145

    摘要： An external ion source assembly in which ion are formed in an ion volume by the interaction of energetic electrons and gas molecules. The effective energy of the electrons entering the ion volume is controlled by changing the voltage between the electron source (filament) and the ionization volume whereby ions having sufficient energy for ionizing atoms and molecules leave the electron source and enter the ionization volume only during an ionization period. The ion source assembly can be used both for electron impact ionization (EI) and for chemical ionization (CI).

    摘要翻译： 外部离子源组件，其中通过能量电子和气体分子的相互作用在离子体中形成离子。 通过改变电子源（细丝）和离子化体积之间的电压来控制进入离子体积的电子的有效能量，由此离子具有足够的电离原子和分子的能量离开电子源并且仅在电离过程中进入离子化体积 期。 离子源组件可用于电子碰撞电离（EI）和化学电离（CI）。

    Ion trap mass spectrometer system and method
    10.
    发明授权
    Ion trap mass spectrometer system and method 失效
    离子阱质谱仪系统及方法

    公开(公告)号：US5420425A

    公开(公告)日：1995-05-30

    申请号：US250156

    申请日：1994-05-27

    申请人： Mark E. Bier ,  John E. P. Syka

    发明人： Mark E. Bier ,  John E. P. Syka

    IPC分类号： G01N27/62 ,  H01J49/34 ,  H01J49/42

    CPC分类号： H01J49/423 ,  H01J49/424

    摘要： The present invention relates generally to an ion trap mass spectrometer for analyzing ions and more particularly to a substantially quadrupole ion trap mass spectrometer with an enlarged ion occupied volume. Described herein are electrode geometries that enlarge the ion occupied volume. Improved ion sensitivities, detection limits and dynamic range should be realized for the same charge density in these devices because the increased ion occupied volume allows for the storage of a greater number of ions. The essence of this invention is that these ion trap geometries may apply all modes of operation of substantially quadrupole ion traps such as the mass selective instability mode, resonance excitation/ejection, and MS.sup.n.

    摘要翻译： 本发明一般涉及一种用于分析离子的离子阱质谱仪，更具体地涉及具有增大的离子占据体积的基本四极离子阱质谱仪。 这里描述了放大离子占据体积的电极几何形状。 对于这些器件中相同的电荷密度，应该实现改进的离子灵敏度，检测限和动态范围，因为增加的离子占据体积允许存储更多数量的离子。 本发明的实质是这些离子阱几何可以应用基本上四极离子阱的所有操作模式，例如质量选择不稳定模式，共振激发/喷射和MSn。