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    Rapid method for preferential coamplification of two different nucleic acid sequences using polymerase chain reaction
    1.
    发明授权
    Rapid method for preferential coamplification of two different nucleic acid sequences using polymerase chain reaction 失效
    使用聚合酶链反应优化共扩增两种不同核酸序列的快速方法

    公开(公告)号：US5674717A

    公开(公告)日：1997-10-07

    申请号：US548078

    申请日：1995-10-25

    申请人： John W. Backus ,  William Harold Donish ,  John Bruce Findlay ,  John William H. Sutherland ,  Marlene M. King

    发明人： John W. Backus ,  William Harold Donish ,  John Bruce Findlay ,  John William H. Sutherland ,  Marlene M. King

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12Q1/70 ,  C12P19/34

    CPC分类号： C12Q1/6851 ,  C12Q1/6848 ,  C12Q1/686

    摘要： Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure in which two different nucleic acid sequences are present. This method allows one to preferentially modulate (for example, suppress) the degree of amplification of one or more nucleic acid sequences relative to other nucleic acid sequences. This modulation is achieved by exploiting differences in the relative primer melt temperatures, or by using certain ratios of primers. Each PCR cycle is very fast, that is less than about 90 seconds. This method is particularly useful for amplification and detection of DNA associated with infectious agents that may be present in a specimen in very small quantities compared to other nontargeted nucleic acids.

    摘要翻译： 可以使用非常快速的聚合酶链反应程序扩增和检测核酸，其中存在两种不同的核酸序列。 该方法允许优先调节（例如，抑制）相对于其它核酸序列的一个或多个核酸序列的扩增程度。 通过利用相对底漆熔融温度的差异，或通过使用一定比例的引物来实现该调节。 每个PCR循环非常快，即小于约90秒。 与其他非靶向核酸相比，该方法特别可用于扩增和检测与可能以非常小的量存在于样品中的感染因子相关联的DNA。

    Diagnostic compositions, elements, methods and test kits for amplification and detection of human CMV DNA using primers having matched melting temperatures
    7.
    发明授权
    Diagnostic compositions, elements, methods and test kits for amplification and detection of human CMV DNA using primers having matched melting temperatures 失效
    使用具有匹配熔融温度的引物扩增和检测人CMV DNA的诊断组合物，元件，方法和测试试剂盒

    公开(公告)号：US06709813B1

    公开(公告)日：2004-03-23

    申请号：US08062021

    申请日：1993-05-14

    申请人： Lynn Bergmeyer ,  Thomas J. Cummins ,  John Bruce Findlay ,  JoAnne H. Kerschner

    发明人： Lynn Bergmeyer ,  Thomas J. Cummins ,  John Bruce Findlay ,  JoAnne H. Kerschner

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6834 ,  C12Q1/686

    摘要： An aqueous composition containing primers for opposing strands of human cytomegaloviral DNA and a second target DNA can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a Tm within the range of from about 65 to about 74° C., while the Tm's are within about 5° C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of hCMV DNA and other target DNA's using multiple capture probes, in “multiplexing”. All of the capture probes have Tm's which are greater than 50° C. and are within 15° C. of each other.

    摘要翻译： 含有针对人类巨细胞病毒DNA和第二靶DNA的引物的含水组合物可以用于聚合酶链式反应中以提供对这些核酸的同时快速和有效的扩增和检测。 每个靶DNA的引物长度不同于5个核苷酸，并且Tm在约65至约74℃的范围内，而Tm在彼此的约5℃内。 这样的组合物可用于诊断测试试剂盒和用于在“多路复用”中使用多个捕获探针扩增和检测hCMV DNA和其他靶DNA的方法。 所有的捕获探针具有大于50℃的Tm，并且彼此在15℃以内。