公开(公告)号：US07670772B2

公开(公告)日：2010-03-02

申请号：US11335939

申请日：2006-01-20

申请人： Joon-ho Kim ,  Jun-hong Min ,  Kyung-hwan Park ,  Soo-suk Lee ,  Hyun-gyu Park

发明人： Joon-ho Kim ,  Jun-hong Min ,  Kyung-hwan Park ,  Soo-suk Lee ,  Hyun-gyu Park

IPC分类号： C12Q1/68 ,  C12M1/00

CPC分类号： B01L3/502707 ,  B01J2219/00522 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00632 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/0072 ,  B01L3/5025 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0887 ,  B82Y30/00

摘要： Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe channels and the sample channels; and forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target sample. Since the probes are immobilized in the microfluidic channels after the formation of the microfluidic channels, difficulty in connecting fluidic channels after the immobilization of probes on a substrate can be removed. In addition, a microfluidic platform for a multiple bioassay into which a plurality of samples can be simultaneously loaded can be formed.

摘要翻译： 提供了用于多重生物测定的微流控芯片及其制造方法。 该方法包括：通过在底表面和基底中耦合具有用于样品通道和探针通道的沟槽的通道结构来形成微流体通道; 在探针通道和样品通道之间的交叉处形成探针固定区域; 并且在每个探针固定区域之前和之后在探针通道中形成阻挡壁以防止靶样品混合。 由于在形成微流体通道之后将探针固定在微流体通道中，因此可以除去在探针固定在基底上之后连接流体通道的困难。 此外，可以形成用于多个生物测定的微流体平台，多个样品可以同时装载到该微生物测定中。

公开(公告)号：US20060188906A1

公开(公告)日：2006-08-24

申请号：US11335939

申请日：2006-01-20

申请人： Joon-ho Kim ,  Jun-hong Min ,  Kyung-hwan Park ,  Soo-suk Lee ,  Hyun-gyu Park

发明人： Joon-ho Kim ,  Jun-hong Min ,  Kyung-hwan Park ,  Soo-suk Lee ,  Hyun-gyu Park

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/502707 ,  B01J2219/00522 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00632 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/0072 ,  B01L3/5025 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0887 ,  B82Y30/00

摘要： Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe channels and the sample channels; and forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target sample. Since the probes are immobilized in the microfluidic channels after the formation of the microfluidic channels, difficulty in connecting fluidic channels after the immobilization of probes on a substrate can be removed. In addition, a microfluidic platform for a multiple bioassay into which a plurality of samples can be simultaneously loaded can be formed.

摘要翻译： 提供了用于多重生物测定的微流控芯片及其制造方法。 该方法包括：通过在底表面和基底中耦合具有用于样品通道和探针通道的沟槽的通道结构来形成微流体通道; 在探针通道和样品通道之间的交叉处形成探针固定区域; 并且在每个探针固定区域之前和之后在探针通道中形成阻挡壁以防止靶样品混合。 由于在形成微流体通道之后将探针固定在微流体通道中，因此可以除去在探针固定在基底上之后连接流体通道的困难。 此外，可以形成用于多个生物测定的微流体平台，多个样品可以同时装载到该微生物测定中。

公开(公告)号：US07510865B2

公开(公告)日：2009-03-31

申请号：US11347185

申请日：2006-02-03

申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

IPC分类号： C12M1/34

CPC分类号： G01N33/54373

摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

公开(公告)号：US20060134777A1

公开(公告)日：2006-06-22

申请号：US11304083

申请日：2005-12-15

申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

IPC分类号： G01N27/447 ,  C12M1/33

CPC分类号： C12N1/066 ,  C12M47/06

摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

公开(公告)号：US07598064B2

公开(公告)日：2009-10-06

申请号：US12349273

申请日：2009-01-06

申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

IPC分类号： C12N13/00 ,  C12N1/06 ,  C12M1/33 ,  C12M1/34 ,  C12Q1/70

CPC分类号： C12N1/066 ,  C12M47/06

摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

公开(公告)号：US20090159463A1

公开(公告)日：2009-06-25

申请号：US12349273

申请日：2009-01-06

申请人： Hun-joo LEE ,  Joon-ho KIM ,  Chang-eun YOO ,  Hee-kyun LIM ,  Kyu-youn Hwang ,  Soo-min MA ,  Jun-hong Min

发明人： Hun-joo LEE ,  Joon-ho KIM ,  Chang-eun YOO ,  Hee-kyun LIM ,  Kyu-youn Hwang ,  Soo-min MA ,  Jun-hong Min

IPC分类号： G01N33/50

CPC分类号： C12N1/066 ,  C12M47/06

摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

摘要翻译： 提供了一种微流体装置，其包括用于细胞裂解的电解装置，其包括阳极室，阴极室和隔板，其中隔膜安装在阳极室和阴极室之间，阳极室包括入口和出口 阳极室溶液和电极，阴极室包括用于阴极室溶液和电极的入口和出口，以及使用其进行电化学裂解细胞的方法。

公开(公告)号：US07981666B2

公开(公告)日：2011-07-19

申请号：US12833095

申请日：2010-07-09

申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

IPC分类号： C12M3/00

CPC分类号： G01N33/54373

摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

公开(公告)号：US07803539B2

公开(公告)日：2010-09-28

申请号：US11297783

申请日：2005-12-07

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H21/00

CPC分类号： C07H21/04

摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

摘要翻译： 提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。 该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。 因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。 此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

公开(公告)号：US20100015695A1

公开(公告)日：2010-01-21

申请号：US12504213

申请日：2009-07-16

申请人： Yoon-kyoung CHO ,  Joon-ho KIM ,  Kak NAMKOONG ,  Geun-bae LIM ,  Jun-hong Min

发明人： Yoon-kyoung CHO ,  Joon-ho KIM ,  Kak NAMKOONG ,  Geun-bae LIM ,  Jun-hong Min

IPC分类号： C12M1/00

CPC分类号： G01N35/08 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0654 ,  B01F13/0071 ,  B01F2015/0221 ,  B01J2219/00783 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2300/0883 ,  G01N2035/00366 ,  G01N2035/00514

摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.

公开(公告)号：US20060177874A1

公开(公告)日：2006-08-10

申请号：US11347185

申请日：2006-02-03

申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim

发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54373

摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.