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    Quantitative fluorescent protein standards
    9.
    发明授权
    Quantitative fluorescent protein standards 有权
    定量荧光蛋白标准品

    公开(公告)号：US08778685B2

    公开(公告)日：2014-07-15

    申请号：US13392493

    申请日：2010-08-25

    申请人： Thomas Diller ,  Timothy Updyke

    发明人： Thomas Diller ,  Timothy Updyke

    IPC分类号： G01N33/68 ,  G01N21/64 ,  G01N27/447

    CPC分类号： G01N33/6803 ,  B33Y80/00 ,  C07K1/26 ,  Y10T436/10 ,  Y10T436/105831

    摘要： The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.

    摘要翻译： 本发明提供了用于同时测定受试蛋白的分子量以及存在于电泳泳道中的受试蛋白的相对质量（即，量）的双重标记蛋白质标准。 本发明还涉及适用于制备这种双重标记的蛋白质标准品的方法以及使用这种双标记蛋白质同时测定受试蛋白质的分子量和相对量的方法。 进一步的实施方案涉及使用双重标记的蛋白质标准物来更准确地测定电泳泳道中存在的蛋白质的量。 另外的实施方案涉及包含目前描述的双重蛋白质标准品的试剂盒。 根据本文所述的实施方案制备和使用的双重标记的蛋白质标准物可用于同时确定受试蛋白质的分子量和相对量。