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1.发明授权
公开(公告)号：US5306862A
公开(公告)日：1994-04-26
申请号：US596467
申请日：1990-10-12
IPC分类号： A01H5/00 , C12N5/10 , C12N9/04 , C12N15/09 , C12N15/53 , C12N15/82 , C12P33/00 , A01H1/04 , A01H1/02 , C12N15/00 , C12P7/22
CPC分类号： C12N9/0006 , C12N15/8243 , C12N15/8286
摘要： A method of increasing sterol accumulation in a plant by increasing the copy number of a gene encoding a polypeptide having HMG-CoA reductase activity is disclosed. The copy number is preferably increased by transforming plants with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide. Also disclosed are a method of increasing cycloartenol accumulation in a plant, a method of increasing the resistance of plants to pests and the transformed plants themselves.
摘要翻译： 公开了通过增加编码具有HMG-CoA还原酶活性的多肽的基因的拷贝数增加植物中甾醇积累的方法。 优选通过用包含可操作地连接到编码具有HMG-CoA还原酶活性的多肽的外源DNA片段的载体的重组DNA分子和适于驱动所述多肽表达的启动子来转化植物来增加拷贝数。 还公开了增加植物中环戊烯醇积累的方法，增加植物对害虫和转化植物本身的抵抗力的方法。
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公开(公告)号：US5365017A
公开(公告)日：1994-11-15
申请号：US119263
申请日：1993-09-09
IPC分类号： A01H5/00 , C12N5/10 , C12N9/04 , C12N15/09 , C12N15/53 , C12N15/82 , C12P33/00 , A01H1/04 , C12N15/00 , C12N9/02 , C07H21/04
CPC分类号： C12N9/0006 , C12N15/8243 , C12N15/8286
摘要： A method of increasing sterol accumulation in a plant by increasing the copy number of a gene encoding a polypeptide having HMG-CoA reductase activity is disclosed. The copy number is preferably increased by transforming plants with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide. Also disclosed are a method of increasing cycloartenol accumulation in a plant, a method of increasing the resistance of plants to pests and the transformed plants themselves.
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3.发明授权Process and composition for increasing squalene and sterol accumulation in higher plants 失效在高等植物中增加角鲨烯和甾醇积累的方法和组合物
公开(公告)号：US5589619A
公开(公告)日：1996-12-31
申请号：US308551
申请日：1994-12-08
申请人： Joseph Chappell , Court A. Saunders , Fred R. Wolf
发明人： Joseph Chappell , Court A. Saunders , Fred R. Wolf
CPC分类号： C12N9/0006 , C12N15/8243 , C12N15/8286
摘要： A process of increasing squalene and sterol accumulation in a transgenic plant by increasing the amount of a gene encoding a polypeptide having HMG-CoA reductase activity is disclosed. The amount is preferably increased by transforming plant cells with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide to form a transformed plant cell and regenerating a transgenic plant from that transformed cell. Also disclosed are a process of increasing pest resistance in a transgenic plant, transgenic plants and transgenic seeds capable of germinating into transgenic plants.
摘要翻译： 公开了通过增加编码具有HMG-CoA还原酶活性的多肽的基因的量来增加转基因植物中角鲨烯和甾醇积累的方法。 优选通过用包含可操作地连接到编码具有HMG-CoA还原酶活性的多肽的外源DNA区段的载体的重组DNA分子和适于驱动所述多肽的表达以形成转化的载体的启动子来转化植物细胞来增加量 植物细胞并从转化细胞再生转基因植物。 还公开了在转基因植物，转基因植物和能够发芽转化植物的转基因种子中增加害虫抗性的方法。
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4.发明授权Process and composition for increasing squalene and sterol accumulation in higher plants 失效在高等植物中增加角鲨烯和甾醇积累的方法和组合物
公开(公告)号：US5349126A
公开(公告)日：1994-09-20
申请号：US934374
申请日：1992-08-14
申请人： Joseph Chappell , Court A. Saunders , Fred R. Wolf
发明人： Joseph Chappell , Court A. Saunders , Fred R. Wolf
IPC分类号： A01H5/00 , C12N5/10 , C12N9/04 , C12N15/09 , C12N15/53 , C12N15/82 , C12P33/00 , A01H1/04 , A01H5/10 , C12N15/00 , C12P21/04
CPC分类号： C12N9/0006 , C12N15/8243 , C12N15/8286
摘要： A process of increasing squalene and sterol accumulation in a transgenic plant by increasing the amount of a gene encoding a polypeptide having HMG-CoA reductase activity is disclosed. The amount is preferably increased by transforming plant cells with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide to form a transformed plant cell and regenerating a transgenic plant from that transformed cell. Also disclosed are a process of increasing pest resistance in a transgenic plant, transgenic plants and transgenic seeds capable of germinating into transgenic plants.
摘要翻译： 公开了通过增加编码具有HMG-CoA还原酶活性的多肽的基因的量来增加转基因植物中角鲨烯和甾醇积累的方法。 优选通过用包含可操作地连接到编码具有HMG-CoA还原酶活性的多肽的外源DNA区段的载体的重组DNA分子和适于驱动所述多肽的表达以形成转化的载体的启动子来转化植物细胞来增加量 植物细胞并从转化细胞再生转基因植物。 还公开了在转基因植物，转基因植物和能够发芽转化植物的转基因种子中增加害虫抗性的方法。
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公开(公告)号：US20140356926A1
公开(公告)日：2014-12-04
申请号：US14121245
申请日：2014-08-13
申请人： Joseph Chappell , Bryan Greenhagen
发明人： Joseph Chappell , Bryan Greenhagen
CPC分类号： C12P5/007 , C12N9/88 , C12Y402/03047 , C12Y402/03073
摘要： The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
摘要翻译： 本发明涉及倍半萜合酶及其生产和使用的方法。 特别地，本发明提供了包含编码至少一个CVS的柑橘类戊烯合酶（CVS）的核苷酸序列的核酸。 本发明还提供了包含编码形成CVS的1级和2级结构域的氨基酸残基的核苷酸序列的核酸。 本发明还提供制备和使用本发明的核酸和氨基酸的方法。
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公开(公告)号：US20130330793A1
公开(公告)日：2013-12-12
申请号：US13986446
申请日：2013-05-03
申请人： Joseph Chappell , Lyle F. Ralston
发明人： Joseph Chappell , Lyle F. Ralston
IPC分类号： C12P7/02
CPC分类号： C12N9/0073 , C12N9/0077 , C12N15/8243 , C12N15/8279 , C12P7/00 , C12P7/02 , C12Y114/13 , C12Y114/13078 , C12Y114/13119
摘要： The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.
摘要翻译： 本发明具有分离的细胞色素P450多肽和核酸分子，以及含有这些分子的表达载体和转基因植物。 此外，本发明的特征在于，在提高转基因植物中由植物病原体引起的疾病的抗性水平，生产改变的化合物的方法，例如羟化化合物的方法中以及在产生类异戊二烯的方法中， 化合物。
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公开(公告)号：US07186891B1
公开(公告)日：2007-03-06
申请号：US09514513
申请日：2000-02-28
申请人： Joseph Chappell , Kyoungwhan Back
发明人： Joseph Chappell , Kyoungwhan Back
CPC分类号： C12N9/88 , C12N9/00 , C12N15/8243
摘要： The invention features plant cells and plants that include a nucleic acid molecule encoding a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned homologous domain. The chimeric isoprenoid synthases of the invention catalyze the production of isoprenoid reaction products that are not produced when the asymmetrically positioned homologous domain is positioned at its naturally-occurring site in an isoprenoid synthase polypeptide.
摘要翻译： 本发明的特征在于植物细胞和植物，其包括编码嵌合型类异戊二烯合酶多肽的核酸分子，其包括不对称定位的同源结构域。 本发明的嵌合类异戊二烯合酶催化产生类异戊二烯反应产物，当不对称定位的同源结构域位于其在异戊二烯合酶多肽中的天然存在位点时不产生。
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8.发明授权Pathogen- or elicitor-inducible transcription regulatory element from the tobacco 5-EPI-aristolochene synthase gene and plants transformed therewith 有权来自烟草5-EPI-aristolochene合酶基因的病原体或诱导子诱导型转录调控元件及其转化的植物公开(公告)号：US06605764B1
公开(公告)日：2003-08-12
申请号：US09435380
申请日：1999-11-05
申请人： Joseph Chappell , Shaohui Yin , Catherine Cornett
发明人： Joseph Chappell , Shaohui Yin , Catherine Cornett
IPC分类号： A01H500
CPC分类号： C12N9/1085 , C07K14/195 , C12N15/8239 , C12N15/8281 , C12N15/8282 , C12N15/8283
摘要： A tobacco epi-5-aristolochene synthase transcriptional regulatory element functional in plants, plant tissue and in plant cells for pathogen inducible gene expression and a method for increasing the transcriptional expression of downstream genetic information in plants, plant tissue and plant cells are disclosed.
摘要翻译： 公开了植物，植物组织和用于病原体诱导型基因表达的植物细胞中功能的烟草外 - 5-aristolochene合酶转录调控元件，以及用于增加植物，植物组织和植物细胞中下游遗传信息的转录表达的方法。
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公开(公告)号：US06569656B2
公开(公告)日：2003-05-27
申请号：US09903012
申请日：2001-07-11
IPC分类号： C12N900
CPC分类号： C12N9/1085 , C12N9/00 , C12N9/88 , C12N15/8243
摘要： Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase, with or without a substrate bound in the active site.
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公开(公告)号：US20100216186A1
公开(公告)日：2010-08-26
申请号：US12259497
申请日：2008-10-28
申请人： Joseph Chappell , Bryan Greenhagen
发明人： Joseph Chappell , Bryan Greenhagen
CPC分类号： C12P5/007 , C12N9/88 , C12Y402/03047 , C12Y402/03073
摘要： The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
摘要翻译： 本发明涉及倍半萜合酶及其生产和使用的方法。 特别地，本发明提供了包含编码至少一个CVS的柑橘类戊烯合酶（CVS）的核苷酸序列的核酸。 本发明还提供了包含编码形成CVS的1级和2级结构域的氨基酸残基的核苷酸序列的核酸。 本发明还提供制备和使用本发明的核酸和氨基酸的方法。
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