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    Methods and kit for protease enzyme assays
    1.
    发明授权
    Methods and kit for protease enzyme assays 有权
    蛋白酶分析方法和试剂盒

    公开(公告)号：US08349580B2

    公开(公告)日：2013-01-08

    申请号：US13394353

    申请日：2010-08-30

    申请人： Joseph J. Stoffel ,  Louis C. Haddad

    发明人： Joseph J. Stoffel ,  Louis C. Haddad

    IPC分类号： C12Q1/37 ,  C12Q1/00 ,  C12N9/76 ,  C12N9/50

    CPC分类号： C12Q1/37 ,  G01N2333/96433 ,  G01N2333/96486 ,  G01N2333/96494 ,  G01N2333/966

    摘要： A method for testing efficacy of a protease enzyme assay, the method comprising providing an enzyme which acts as a surrogate enzyme control for the protease enzyme; combining the surrogate enzyme control with an assay substrate for the protease enzyme; and determining a change in the assay substrate resulting from the surrogate enzyme control acting on the assay substrate; wherein the protease enzyme is selected from the group consisting of metalloproteinases, serine proteases, and cysteine proteases; a method for conducting a protease enzyme assay using the method for testing efficacy; a kit including an enzyme which acts as a surrogate enzyme control for a protease enzyme in testing efficacy of a protease enzyme assay; and a method of releasing the kit are provided.

    摘要翻译： 一种用于测试蛋白酶测定的功效的方法，所述方法包括提供用作蛋白酶的替代酶对照的酶; 将替代酶控制与用于蛋白酶的测定底物组合; 以及确定由作用在测定底物上的替代酶控制产生的测定底物的变化; 其中所述蛋白酶选自金属蛋白酶，丝氨酸蛋白酶和半胱氨酸蛋白酶; 使用测试功效的方法进行蛋白酶测定的方法; 一种试剂盒，其包括在测试蛋白酶测定的功效中用作蛋白酶的替代酶对照的酶; 并提供了释放试剂盒的方法。

    METHODS AND KIT FOR PROTEASE ENZYME ASSAYS
    2.
    发明申请
    METHODS AND KIT FOR PROTEASE ENZYME ASSAYS 有权
    蛋白酶测定方法和工具包

    公开(公告)号：US20120164675A1

    公开(公告)日：2012-06-28

    申请号：US13394353

    申请日：2010-08-30

    申请人： Joseph J. Stoffel ,  Louis C. Haddad

    发明人： Joseph J. Stoffel ,  Louis C. Haddad

    IPC分类号： C12Q1/37 ,  G01N21/59 ,  G01N21/64

    CPC分类号： C12Q1/37 ,  G01N2333/96433 ,  G01N2333/96486 ,  G01N2333/96494 ,  G01N2333/966

    摘要： A method for testing efficacy of a protease enzyme assay, the method comprising providing an enzyme which acts as a surrogate enzyme control for the protease enzyme; combining the surrogate enzyme control with an assay substrate for the protease enzyme; and determining a change in the assay substrate resulting from the surrogate enzyme control acting on the assay substrate; wherein the protease enzyme is selected from the group consisting of metalloproteinases, serine proteases, and cysteine proteases; a method for conducting a protease enzyme assay using the method for testing efficacy; a kit including an enzyme which acts as a surrogate enzyme control for a protease enzyme in testing efficacy of a protease enzyme assay; and a method of releasing the kit are provided.

    摘要翻译： 一种用于测试蛋白酶测定的功效的方法，所述方法包括提供用作蛋白酶的替代酶对照的酶; 将替代酶控制与用于蛋白酶的测定底物组合; 以及确定由作用在测定底物上的替代酶控制产生的测定底物的变化; 其中所述蛋白酶选自金属蛋白酶，丝氨酸蛋白酶和半胱氨酸蛋白酶; 使用测试功效的方法进行蛋白酶测定的方法; 一种试剂盒，其包括在测试蛋白酶测定的功效中用作蛋白酶的替代酶对照的酶; 并提供了释放试剂盒的方法。

    Sample acquisition device
    3.
    发明授权
    Sample acquisition device 失效
    样品采集装置

    公开(公告)号：US08677843B2

    公开(公告)日：2014-03-25

    申请号：US12867146

    申请日：2009-02-10

    申请人： Bernard A. Gonzalez ,  G. Marco Bommarito ,  Paul J. Cobian ,  Tera M. Nordby ,  Tushar A. Kshirsagar ,  Scott A. Burton ,  Joseph J. Stoffel ,  Patrick A. Mach ,  Gustavo H. Castro

    发明人： Bernard A. Gonzalez ,  G. Marco Bommarito ,  Paul J. Cobian ,  Tera M. Nordby ,  Tushar A. Kshirsagar ,  Scott A. Burton ,  Joseph J. Stoffel ,  Patrick A. Mach ,  Gustavo H. Castro

    IPC分类号： G01N1/10

    CPC分类号： G01N1/02 ,  A61B10/0045 ,  G01N2001/028

    摘要： A sample acquisition device includes a capillary array configured to draw in a sample and retain the sample by capillary action. The capillary array may be coupled to an elongated member, such as a stem or a hollow tube, which defines a longitudinal axis extending in a first direction. In some embodiments, the capillary array defines a major sample acquisition surface that extends in a second direction different than the first direction. A ratio of the major sample acquisition surface area to maximum volume retained by the capillary array may be selected to minimize physical binding between the capillary array and sample. In some embodiments, the device may include a feedback mechanism to indicate the relative pressure applied to a sample source with the sample acquisition device. In addition, in some embodiments, the sample acquisition device may include a suction source to help draw the sample into the capillary array.

    摘要翻译： 样品采集装置包括毛细管阵列，该毛细管阵列构造成吸取样品并通过毛细管作用保留样品。 毛细管阵列可以耦合到细长构件，例如杆或中空管，其限定沿第一方向延伸的纵向轴线。 在一些实施例中，毛细管阵列限定沿与第一方向不同的第二方向延伸的主要样本采集表面。 可以选择毛细管阵列保留的主要样品采集表面积与最大体积的比率以使毛细管阵列和样品之间的物理结合最小化。 在一些实施例中，装置可以包括反馈机构，以指示使用样本采集装置施加到样本源的相对压力。 此外，在一些实施例中，样品采集装置可以包括抽吸源以帮助将样品吸入毛细管阵列。

    SAMPLE ACQUISITION DEVICE
    6.
    发明申请
    SAMPLE ACQUISITION DEVICE 失效
    样本获取设备

    公开(公告)号：US20110146419A1

    公开(公告)日：2011-06-23

    申请号：US12867146

    申请日：2009-02-10

    申请人： Bernard A. Gonzalez ,  G. Marco Bommarito ,  Paul J. Cobian ,  Tera M. Nordby ,  Tushar A. Kshirsagar ,  Scott A. Burton ,  Joseph J. Stoffel ,  Patrick A. Mach ,  Gustavo H. Castro

    发明人： Bernard A. Gonzalez ,  G. Marco Bommarito ,  Paul J. Cobian ,  Tera M. Nordby ,  Tushar A. Kshirsagar ,  Scott A. Burton ,  Joseph J. Stoffel ,  Patrick A. Mach ,  Gustavo H. Castro

    IPC分类号： B01L3/02 ,  G01N1/14

    CPC分类号： G01N1/02 ,  A61B10/0045 ,  G01N2001/028

    摘要： A sample acquisition device includes a capillary array configured to draw in a sample and retain the sample by capillary action. The capillary array may be coupled to an elongated member, such as a stem or a hollow tube, which defines a longitudinal axis extending in a first direction. In some embodiments, the capillary array defines a major sample acquisition surface that extends in a second direction different than the first direction. A ratio of the major sample acquisition surface area to maximum volume retained by the capillary array may be selected to minimize physical binding between the capillary array and sample. In some embodiments, the device may include a feedback mechanism to indicate the relative pressure applied to a sample source with the sample acquisition device. In addition, in some embodiments, the sample acquisition device may include a suction source to help draw the sample into the capillary array.

    摘要翻译： 样品采集装置包括毛细管阵列，该毛细管阵列构造成吸取样品并通过毛细管作用保留样品。 毛细管阵列可以耦合到细长构件，例如杆或中空管，其限定沿第一方向延伸的纵向轴线。 在一些实施例中，毛细管阵列限定沿与第一方向不同的第二方向延伸的主要样本采集表面。 可以选择毛细管阵列保留的主要样品采集表面积与最大体积的比率以使毛细管阵列和样品之间的物理结合最小化。 在一些实施例中，装置可以包括反馈机构，以指示使用样本采集装置施加到样本源的相对压力。 此外，在一些实施例中，样品采集装置可以包括抽吸源以帮助将样品吸入毛细管阵列。