摘要:
The invention is directed to methods for direct patterning of silicon. The invention provides the ability to fabricate complex surfaces in silicon with three dimensional features of high resolution and complex detail. The invention is suitable, for example, for use in soft lithography as embodiments of the invention can quickly create a master for use in soft lithography. In an embodiment of the invention, electrochemical etching of silicon, such as a silicon wafer, for example, is conducted while at least a portion of the silicon surface is exposed to an optical pattern. The etching creates porous silicon in the substrate, and removal of the porous silicon layer leaves a three-dimensional structure correlating to the optical pattern.
摘要:
The invention is directed to methods for direct patterning of silicon. The invention provides the ability to fabricate complex surfaces in silicon with three dimensional features of high resolution and complex detail. The invention is suitable, for example, for use in soft lithography as embodiments of the invention can quickly create a master for use in soft lithography. In an embodiment of the invention, electrochemical etching of silicon, such as a silicon wafer, for example, is conducted while at least a portion of the silicon surface is exposed to an optical pattern. The etching creates porous silicon in the substrate, and removal of the porous silicon layer leaves a three-dimensional structure correlating to the optical pattern.
摘要:
Provided is a microarray platform for the culture of cells atop combinatorial matrix mixtures; enabling the study of differentiation in response to a multitude of microenvironments in parallel.
摘要:
Any of inorganic and bio-organic substances and molecules, and beads, pucks and like small things, that are both (i) electrically charged to a first polarity, and (ii) immersed in a fluid transport medium within (iii) an electrochemical cell, are assembled and patterned by action of moving these inorganic and bio-organic substances and molecules, etc. to a patterned electrode having an opposite, second, polarity under force of an applied electric field. The electrode patterned with conductive areas may be further, separately, patterned with chemicals, for example agarose gel, that chemically accept or reject the substances and molecules, etc., especially as are biological in origin. Living cells of plant, bacterial and animal types may be assembled. A semiconductor electrode may be patterned by masked laser light passed through the other electrode, which is transparent.
摘要:
The invention includes sensors and sensing methods for determining cell morphology and/or chemical composition of an analyte. A porous substrate exhibiting a first optical signal is exposed to a target analyte and subsequently monitored for changes in the optical signal. More specifically, a photonic or porous substrate having a well-defined and highly tunable reflectivity or transmission spectrum, such as porous silicon (Si), porous alumina, porous Ge, porous GaAs, porous SiO2 and porous polymer, is used for example. A porous or photonic substrate is exposed to an analyte, such as a cell or other macromolecule, and changes in the scattered light are observed over time to determine cell morphology and/or chemical composition of the analyte using the substrate.
摘要:
The invention includes sensors and sensing methods for determining cell morphology and/or chemical composition of an analyte. A porous substrate exhibiting a first optical signal is exposed to a target analyte and subsequently monitored for changes in the optical signal. More specifically, a photonic or porous substrate having a well-defined and highly tunable reflectivity or transmission spectrum, such as porous silicon (Si), porous alumina, porous Ge, porous GaAs, porous SiO2 and porous polymer, is used for example. A porous or photonic substrate is exposed to an analyte, such as a cell or other macromolecule, and changes in the scattered light are observed over time to determine cell morphology and/or chemical composition of the analyte using the substrate.
摘要:
The disclosure provides a bioreactor that allows steady-state oxygen gradients to be imposed upon in vitro culture systems. The bioreactor system of the disclosure has been applied to liver zonation and have shown that physiological oxygen gradients contribute to heterogeneity of tissue cultures in vitro.
摘要:
Provided are methods of the production of patterned 3-dimesional biopolymer scaffolds containing living cells. The methods include selective photopolymerization of biopolymers to create patterned structures and the patterning of cells within relatively homogenous slabs of biopolymer using dielectrophoresis. Also provided are patterned 3-dimensional biopolymer scaffolds generated by the methods and their use.
摘要:
The invention is a series of soft lithographic methods for the microfabrication of biopoilymer scaffolds for use in tissue engineering and the development of artificial organs. The methods present a wide range of possibilities to construct two- and three-dimensional scaffolds with desired characteristics according to the final application. The methods utilize an elastomer mold which the biopolymer scaffold is cast. The methods allow for the rapid and inexpensive production of biopolymer scaffolds with limited specialized equipment and user expertise.