公开(公告)号：US06825016B1

公开(公告)日：2004-11-30

申请号：US09958515

申请日：2001-10-09

申请人： Kaori Ishimaru ,  Masayuki Yagi ,  Satoshi Yonehara

发明人： Kaori Ishimaru ,  Masayuki Yagi ,  Satoshi Yonehara

IPC分类号： C12N900

CPC分类号： C12R1/01 ,  C12N9/48

摘要： The present invention relates to a novel enzyme (&agr;-GARE) which releases an amino acid residue having a glycated &agr;-amino group (&agr;-GA) from a glycated protein etc. and to bacterial strains producing the same. Examples of the bacterial strains include Sphingomonas parapaucimobilis KDK1004 (FERM BP-7041). The &agr;-GARE is contained in the culture supernatant of this strain and &agr;-GA can be released from a glycated peptide by using the same, as shown in FIG. 1.

摘要翻译： 本发明涉及从糖化蛋白质等释放具有糖化α-氨基（α-GA）的氨基酸残基的新型酶（α-GARE）和产生该氨基酸残基的细菌菌株。 细菌菌株的实例包括鞘氨醇单胞菌（Parpaucimobilis）KDK1004（FERM BP-7041）。 α-GARE包含在该菌株的培养上清液中，并且通过使用α-GA可以从糖化肽释放α-GA，如图1所示。 1。

公开(公告)号：US06797503B1

公开(公告)日：2004-09-28

申请号：US09913874

申请日：2001-10-11

申请人： Kaori Ishimaru ,  Masayuki Yagi ,  Satoshi Yonehara

发明人： Kaori Ishimaru ,  Masayuki Yagi ,  Satoshi Yonehara

IPC分类号： C12N952

CPC分类号： C12R1/15 ,  C12N9/48 ,  C12R1/38

摘要： The present invention relates to a novel enzyme (&agr;-GARE) which releases an amino acid residue having a glycated &agr;-amino group (&agr;-GA) from a glycated protein etc. and to bacterial strains producing the same. Examples of the bacterial strains include Corynebacterium ureolyticum KDK1002 (FERM P-17135) and Pseudomonas alcaligenes KDK1001 (FERM P-17133). The &agr;-GARE is contained in the culture supernatant of these strains and &agr;-GA can be released from a glycated peptide by using the same, as shown in FIG. 1.

摘要翻译： 本发明涉及从糖化蛋白质等释放具有糖化α-氨基（α-GA）的氨基酸残基的新型酶（α-GARE）和产生该氨基酸残基的细菌菌株。 细菌菌株的实例包括溶脲杆菌KDK1002（FERM P-17135）和产碱假单胞菌KDK1001（FERM P-17133）。 α-GARE包含在这些菌株的培养上清液中，并且通过使用α-GA可以从糖化肽释放α-GA，如图1所示。 1。

公开(公告)号：US6033867A

公开(公告)日：2000-03-07

申请号：US77523

申请日：1998-07-29

申请人： Nobuo Kato ,  Yasuyoshi Sakai ,  Yoshiki Tani ,  Hiroshi Fukuya ,  Masayuki Yagi ,  Toshikatsu Sakai ,  Kaori Ishimaru ,  Satoshi Cyousa

发明人： Nobuo Kato ,  Yasuyoshi Sakai ,  Yoshiki Tani ,  Hiroshi Fukuya ,  Masayuki Yagi ,  Toshikatsu Sakai ,  Kaori Ishimaru ,  Satoshi Cyousa

IPC分类号： C12N9/06 ,  C12Q1/26 ,  C12N1/14 ,  C12N9/04 ,  C12N9/62

CPC分类号： C12N9/0022 ,  C12Q1/26 ,  G01N2333/906 ,  G01N2333/90633

摘要： The present invention relates to a fructosyl amino acid oxidase that is obtainable from a culture of a strain of the genus Aspergillus. This strain grows in a selective medium containing at least either of fructosyl lysine and fructosyl-N.sup..alpha. -Z-lysine and is capable of producing a fructosyl amino acid oxidase.

摘要翻译： PCT No.PCT / JP96 / 03515 Sec。 371日期：1998年5月29日 102（e）日期1989年5月29日PCT 1996年12月2日PCT公布。 公开号WO97 / 20039 日期：1997年6月5日本发明涉及可从曲霉属菌株的培养物获得的果糖基氨基酸氧化酶。 该菌株在含有果糖基赖氨酸和果糖基-Nα-Z-赖氨酸中的至少一种的选择性培养基中生长，并且能够产生果糖基氨基酸氧化酶。

公开(公告)号：US20130040314A1

公开(公告)日：2013-02-14

申请号：US13566169

申请日：2012-08-03

申请人： Satoko Togashi ,  Masayuki Yagi

发明人： Satoko Togashi ,  Masayuki Yagi

IPC分类号： G01N33/545 ,  C07C275/00 ,  G01N30/00 ,  G01N33/53 ,  G01N33/553

CPC分类号： G01N33/6812 ,  G01N33/54313

摘要： A measuring method by which carboxymethylarginine can be measured with ease and with a high degree of precision is disclosed. The measuring method is a method of measuring carboxymethylarginine in a sample. The method includes treating the sample with a pretreatment agent including urea; and measuring carboxymethyl arginine in the pretreated sample by an immunological method.

摘要翻译： 公开了一种容易且高精度地测定羧甲基精氨酸的测定方法。 测量方法是测量样品中的甲基精氨酸的方法。 该方法包括用包括尿素的预处理剂处理样品; 并用免疫学方法测定预处理样品中的羧甲基精氨酸。

公开(公告)号：US20100009016A1

公开(公告)日：2010-01-14

申请号：US12307667

申请日：2008-01-30

申请人： Masayuki Yagi ,  Hiroshige Kawal ,  Motoki Kubo ,  Yoshikazu Yonei ,  Nobuyasu Matsuura

发明人： Masayuki Yagi ,  Hiroshige Kawal ,  Motoki Kubo ,  Yoshikazu Yonei ,  Nobuyasu Matsuura

IPC分类号： A61K36/00

CPC分类号： A61K36/734 ,  A61K8/02 ,  A61K8/97 ,  A61K36/185 ,  A61K36/28 ,  A61K36/87 ,  A61K2800/92 ,  A61Q19/08

摘要： A new orally administerable DHEA production promoter is provided. A composition containing an extract of at least one selected from the group consisting of plants of Rosaceae Crataegus, Saururaceae Houttuynia, Vitaceae Vitis, and Compositae Anthemis is provided as a DHEA production promoter. The extract may be of one of the plants or extracts of two or more of them may be used in combination. A specific example is preferably a mixture of extracts of all four of the aforementioned plants. This DHEA production promoter also can be used as, for example, an anti-aging agent such as a skin improver as well as a pharmaceutical agent such as an immunostimulator, an antidiabetic agent, an anti-osteoporosis agent, an antiarteriosclerotic agent, an anti-obesity agent, a sleep-promoting agent, and a central nervous system depressant.

摘要翻译： 提供了一种新的可口服的DHEA生产促进剂。 作为DHEA生产促进剂，提供了含有选自蔷薇科，禾本科鱼腥草，芸苔科和菊科植物的植物中的至少一种的提取物的组合物。 提取物可以是其中一种植物或其两种或更多种的提取物可以组合使用。 具体实例优选为所有四种上述植物的提取物的混合物。 该DHEA生产促进剂也可以用作例如抗衰老剂如皮肤改良剂，以及药剂如免疫刺激剂，抗糖尿病剂，抗骨质疏松剂，抗动脉硬化剂，抗 - - 肥胖剂，睡眠促进剂和中枢神经系统抑制剂。

公开(公告)号：US5712167A

公开(公告)日：1998-01-27

申请号：US682219

申请日：1996-07-17

申请人： Yoshinori Yamaguchi ,  Xiaoming Dou ,  Masayuki Yagi ,  Harumi Uenoyama

发明人： Yoshinori Yamaguchi ,  Xiaoming Dou ,  Masayuki Yagi ,  Harumi Uenoyama

IPC分类号： G01N21/65 ,  G01N21/47

CPC分类号： G01N21/65 ,  Y10T436/173845

摘要： In order to quantitatively measure an Amadori compound by a simple optical method utilizing light scattering, a sample containing an Amadori compound which is stored in a cell is irradiated with excitation light from an He--Ne laser unit so that scattered light from the sample is received and separated into its spectral components for obtaining a light scattering spectrum, and a light scattering peak existing at 820 to 840 cm.sup.-1, 1655 to 1660 cm.sup.-1, 2000 to 2020 cm.sup.-1, 2080 to 2100 cm.sup.-1, 2460 to 2470 cm.sup.-1 or 2530 to 2600 cm.sup.-1 in shift wavenumber with respect to the excitation wavelength in the light scattering spectrum is detected by a detector. The saccharide concentration or the saccharification ratio of the Amadori compound is measured by a calibration curve through the peak intensity or the peak integral value of the light scattering peak.

摘要翻译： 为了通过使用光散射的简单光学方法定量测量阿马多利化合物，将来自He-Ne激光单元的激发光照射存储在电池中的含有Amadori化合物的样品，从而接收来自样品的散射光 并分离成其光谱分量以获得光散射光谱，以及存在于820至840cm -1，1655至1660cm -1，2000至2020cm -1，2080至2100cm -1，2460至 通过检测器检测相对于光散射光谱中的激发波长的2470cm -1或2530〜2600cm -1的位移波数。 阿马多利化合物的糖浓度或糖化率通过校准曲线通过峰光强度或光散射峰的峰值积分值来测量。

公开(公告)号：US5158724A

公开(公告)日：1992-10-27

申请号：US521360

申请日：1990-05-10

申请人： Masayuki Yagi ,  Toshihiko Kusago

发明人： Masayuki Yagi ,  Toshihiko Kusago

IPC分类号： B29C43/24 ,  B29C43/34 ,  B29C43/58 ,  B29C47/92 ,  G01N11/00

CPC分类号： B29C47/92 ,  B29C43/24 ,  B29C43/245 ,  B29C47/0021 ,  G01N11/00 ,  B29C2947/92076 ,  B29C2947/92152 ,  B29C2947/92161 ,  B29C2947/92209 ,  B29C2947/92428 ,  B29C2947/92447 ,  B29C2947/9259 ,  B29C2947/926 ,  B29C2947/92647 ,  B29C2947/92657 ,  B29C2947/92704 ,  B29C2947/92885 ,  B29C2947/92904 ,  B29C2947/92923

摘要： A plastic material, controlled at a desired temperature, is fed on an upstream side of a gap between a pair of rotating forming rolls arrayed almost in parallel and controlled at a desired temperature from a flat die connected to an extruding machine and having a slit outflow portion. The plastic material is pressed and formed into a sheet by the forming rolls therebetween while forming a bank or an accumulation of the plastic material on an upstream side of the gap of the forming rolls and is carried out on a downstream side of the gap. The bank quantity monitoring is performed by measuring a temperature of the sheet carried out through the gap of the forming rolls and estimating the quantity of the bank in accordance with measured temperature information. The sheet formation is performed by measuring a temperature of the sheet carried out through the gap of the forming rolls and controlling various forming conditions according to a measured temperature information. The sheet temperature measurement is performed by measuring a temperature of the sheet by directing said non-contacted temperature measuring means against an inner part of a wedge-shaped space formed by a surface of the sheet carried out of the gap and a surface of the forming roll facing on the surface of the sheet at a slight gap therebetween.

摘要翻译： 控制在期望温度的塑料材料被馈送在一对旋转成形辊（11,12）之间的间隙（13）的上游侧，该对旋转成形辊几乎平行地排列并且从连接的扁平模具（10）被控制在所需温度 到挤出机并且具有狭缝流出部分，塑料材料通过其间的成形辊（11,12）被挤压并形成为片材（15），同时形成堤（14a）或塑料材料的堆积在 成形辊的间隙（13）的上游侧，并且在间隙的下游侧进行。 通过测量通过成形辊（11,12）的间隙（13）进行的片材（15）的温度并根据测量的温度信息来估计存储体（14a）的量来进行库数量监视。 通过测量通过成形辊（11,12）的间隙（13）进行的片材的温度并根据测量的温度信息控制各种成形条件来进行片材形成。

公开(公告)号：US5972671A

公开(公告)日：1999-10-26

申请号：US30442

申请日：1998-02-25

申请人： Nobuo Kato ,  Yasuyoshi Sakai ,  Yoshiki Tani ,  Masayuki Yagi ,  Fumiyo Funatsu

发明人： Nobuo Kato ,  Yasuyoshi Sakai ,  Yoshiki Tani ,  Masayuki Yagi ,  Fumiyo Funatsu

IPC分类号： C12N1/26 ,  C12N9/06 ,  C12Q1/26 ,  C12N1/14 ,  C12N9/02 ,  C12N9/04

CPC分类号： C12N9/0022 ,  C12N1/26 ,  C12Q1/26 ,  C12R1/77 ,  Y10S435/929

摘要： A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.

摘要翻译： 来自镰刀菌属或赤霉属的新型果糖基氨基酸氧化酶，生产酶的方法，使用该酶的阿马多利化合物的试验，含有酶的试剂或试剂盒，生产果糖基赖氨酸和/或果糖基N的方法 作为果糖基氨基酸氧化酶底物的α-Z-赖氨酸，其可用于筛选和/或培养能够产生该酶的微生物，以及含有果糖基赖氨酸和/或果糖基Nα-Z-赖氨酸的培养基用于筛选 和/或培养能产生果糖基氨基酸氧化酶的微生物。

公开(公告)号：US20100240603A1

公开(公告)日：2010-09-23

申请号：US12161229

申请日：2007-06-19

申请人： Masayuki Yagi ,  Nobuyasu Matsuura

发明人： Masayuki Yagi ,  Nobuyasu Matsuura

IPC分类号： A61K31/7048 ,  C07H15/24 ,  A61P35/00 ,  A61P9/10 ,  A61P9/12 ,  A61P3/10 ,  A61P17/00 ,  A61P37/00 ,  A61P19/02 ,  A61P11/06 ,  A61P37/08 ,  A61P29/00 ,  A61P25/00 ,  A61P25/16 ,  A61P25/28 ,  A61P17/10 ,  A61P17/06

CPC分类号： A61K36/28 ,  A23L33/105 ,  A61K31/7048

摘要： An expression inhibitor of a nuclear transcription factor AP-1 is provided that is excellent in safety and activity of inhibiting the expression of a nuclear transcription factor AP-1. The AP-1 expression inhibitor of the present invention is characterized by containing chamaemeloside. In the present invention, chamaemeloside may be contained as an extract of Roman chamomile or German chamomile. The chamaemeloside is contained in Roman chamomile or German chamomile. Conventionally, Roman chamomile and German chamomile have been used as cosmetic materials and herb teas and have no problems in safety. An extract of Roman chamomile or German chamomile and chamaemeloside inhibit the expression of the AP-1 at the gene level (see FIG. 2) and therefore are excellent in an inhibition effect as compared to a conventional inhibitor that inhibits binding of the AP-1 to DNA.

摘要翻译： 提供了核转录因子AP-1的表达抑制剂，其具有优异的安全性和抑制核转录因子AP-1表达的活性。 本发明的AP-1表达抑制剂的特征在于含有chamaemeloside。 在本发明中，作为罗马洋甘菊或德国洋甘菊的提取物，可以含有沙门氏菌素。 chamaemeloside含有罗马洋甘菊或德国洋甘菊。 通常，罗马洋甘菊和德国洋甘菊已经被用作化妆品和草药茶，没有安全问题。 罗马洋甘菊或德国洋甘菊和chamaemeloside的提取物抑制AP-1在基因水平的表达（参见图2），因此与抑制AP-1结合的常规抑制剂相比，具有优异的抑制作用 到DNA。

公开(公告)号：US20100229790A1

公开(公告)日：2010-09-16

申请号：US12740816

申请日：2008-10-30

申请人： Mitsuaki Serizawa ,  Masayuki Yagi

发明人： Mitsuaki Serizawa ,  Masayuki Yagi

IPC分类号： B05D3/12 ,  B29C59/04

CPC分类号： B29C59/046 ,  B29C59/026 ,  B29C2035/0827 ,  B82Y10/00 ,  B82Y40/00 ,  G03F7/0002 ,  G03F7/2035 ,  G11B7/26 ,  G11B7/263 ,  G11B7/265

摘要： An intermittent coating member 10 delivers resin intermittently from a coating die 20 onto a length of base film 41 fed forth from a feeding member 40 to form intermittent coats of a coating film 43 on the base film 41. The coating film 43 is transferred to a transfer member 44 that actuates in synchronism with the coating action for the coating film 43, and is given a transfer by moulds 45 fixed on a mould roll 46. After the transfer, the coating film 43 is cured by an ultraviolet irradiation device 48 and wound up by a winding member 49.

摘要翻译： 间歇涂布部件10将树脂从涂布模具20间歇地输送到从供给部件40供给的基膜41的长度上，在基膜41上形成涂膜43的间歇涂层。将涂膜43转印到 转印构件44，其与涂膜43的涂布动作同步地进行驱动，并且通过固定在模制辊46上的模具45进行转印。转印后，涂膜43通过紫外线照射装置48固化并卷绕 通过绕组构件49。