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1.发明授权Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme 失效使用热稳定酶扩增，检测和/或克隆核酸序列的方法
公开(公告)号：US4965188A
公开(公告)日：1990-10-23
申请号：US63647
申请日：1987-06-17
CPC分类号： C12P19/34 , C12N9/1252 , C12Q1/6844
摘要： A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译： 用于扩增核酸或其混合物中包含的任何靶核酸序列的方法包括用摩尔过量的两个寡核苷酸引物处理所述核酸的分开的互补链，并用热稳定酶扩增引物以形成互补引物延伸产物，其起作用 作为合成所需核酸序列的模板。 可以容易地检测扩增的序列。 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。
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公开(公告)号：US06197563B1
公开(公告)日：2001-03-06
申请号：US08345367
申请日：1994-11-18
IPC分类号： C12N912
CPC分类号： C12P19/34 , B01J19/0046 , B01J2219/00495 , B01J2219/0059 , B01J2219/00689 , B01J2219/00722 , B01L7/52 , C07H21/00 , C07K14/70539 , C07K14/805 , C12N9/1252 , C12N15/10 , C12Q1/6846 , C40B40/06 , C40B60/14
摘要： The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译： 本发明涉及使用热稳定性酶扩增核酸或其混合物中包含的任何靶核酸序列的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的分开的互补链，用热稳定酶扩增引物以形成互补引物延伸产物，其用作合成所需核酸序列的模板，并检测序列 放大 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。
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公开(公告)号：US06514736B1
公开(公告)日：2003-02-04
申请号：US09704357
申请日：2000-11-01
IPC分类号： C12N912
CPC分类号： C12Q1/6858 , B01J19/0046 , B01J2219/0059 , B01J2219/00722 , B01L7/52 , C07K14/70539 , C07K14/805 , C12N9/1252 , C12N15/10 , C12P19/34 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , C40B40/06 , Y10S977/92 , C12Q2549/119 , C12Q2525/149 , C12Q2525/143 , C12Q2525/131
摘要： The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译： 本发明涉及使用热稳定性酶扩增核酸或其混合物中包含的任何靶核酸序列的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的分开的互补链，用热稳定酶扩增引物以形成互补引物延伸产物，其用作合成所需核酸序列的模板，并检测序列 放大 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。
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4.发明授权
公开(公告)号：US6040166A
公开(公告)日：2000-03-21
申请号：US312729
申请日：1994-09-27
IPC分类号： B01J19/00 , B01L7/00 , C07K14/74 , C07K14/805 , C12N9/12 , C12N15/10 , C12P19/34 , C12Q1/68 , C40B40/06
CPC分类号： C12Q1/6858 , B01J19/0046 , B01L7/52 , C07K14/70539 , C07K14/805 , C12N15/10 , C12N9/1252 , C12P19/34 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , B01J2219/0059 , B01J2219/00722 , C40B40/06 , Y10S977/92
摘要： The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译： 本发明涉及使用热稳定性酶扩增核酸或其混合物中包含的任何靶核酸序列的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的分开的互补链，用热稳定酶扩增引物以形成互补引物延伸产物，其用作合成所需核酸序列的模板，并检测序列 放大 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。
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公开(公告)号：US07445900B2
公开(公告)日：2008-11-04
申请号：US11203461
申请日：2005-08-11
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815 , C12Q2521/101 , C12Q2565/1015 , C12Q2531/113 , C12Q2521/319 , C12Q2535/131 , C12Q2521/325 , C12Q2537/157 , C12Q2561/101 , C12Q2525/186 , C12Q2525/131
摘要： A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.
摘要翻译： 使用标记的寡核苷酸检测靶核酸的过程使用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火标记的寡核苷酸，并释放标记的寡核苷酸片段进行检测。 该方法容易地并入PCR扩增测定中。
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公开(公告)号：US20080171315A1
公开(公告)日：2008-07-17
申请号：US11203461
申请日：2005-08-11
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815 , C12Q2521/101 , C12Q2565/1015 , C12Q2531/113 , C12Q2521/319 , C12Q2535/131 , C12Q2521/325 , C12Q2537/157 , C12Q2561/101 , C12Q2525/186 , C12Q2525/131
摘要： A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.
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公开(公告)号：US06214979B1
公开(公告)日：2001-04-10
申请号：US08934378
申请日：1997-09-19
IPC分类号： C07H2100
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815 , C12Q2521/101 , C12Q2565/1015 , C12Q2531/113 , C12Q2521/319 , C12Q2535/131 , C12Q2521/325 , C12Q2537/157 , C12Q2561/101 , C12Q2525/186 , C12Q2525/131
摘要： A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
摘要翻译： 使用标记的寡核苷酸检测靶核酸的过程使用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火标记的寡核苷酸，并释放标记的寡核苷酸片段进行检测。 该方法容易地并入PCR扩增测定中。
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8.发明授权Nucleic acid detection by the 5'-3'exonuclease activity of polymerases acting on adjacently hybridized oligonucleotides 失效通过作用于相邻杂交寡核苷酸的聚合酶的5'-3核酸外切酶活性检测核酸
公开(公告)号：US5487972A
公开(公告)日：1996-01-30
申请号：US961884
申请日：1993-01-05
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815
摘要： A process of detecting a target nucleic acid using labeled oligonucleotides which uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and thus releasing labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
摘要翻译： 使用使用核酸聚合酶的5'至3'核酸酶活性的标记的寡核苷酸检测靶核酸的过程，以从杂交的双链体切割退火的标记寡核苷酸，从而释放用于检测的标记的寡核苷酸片段。 该方法容易地并入PCR扩增测定中。
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9.发明授权Homogeneous assay system using the nuclease activity of a nucleic acid polymerase 失效使用核酸聚合酶的核酸酶活性的均质测定系统
公开(公告)号：US5210015A
公开(公告)日：1993-05-11
申请号：US563758
申请日：1990-08-06
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815
摘要： The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
摘要翻译： 本发明涉及使用标记的寡核苷酸检测靶核酸的方法。 该方法使用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火标记的寡核苷酸并释放标记的寡核苷酸片段进行检测。 该方法容易地并入PCR扩增测定中。
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公开(公告)号：US07141377B2
公开(公告)日：2006-11-28
申请号：US11043099
申请日：2005-01-27
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815 , C12Q2521/101 , C12Q2565/1015 , C12Q2531/113 , C12Q2521/319 , C12Q2535/131 , C12Q2521/325 , C12Q2537/157 , C12Q2561/101 , C12Q2525/186 , C12Q2525/131
摘要： A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
摘要翻译： 使用标记的寡核苷酸检测靶核酸的过程使用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火标记的寡核苷酸，并释放标记的寡核苷酸片段进行检测。 该方法容易地并入PCR扩增测定中。
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