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    Water Filtration System
    3.
    发明申请
    Water Filtration System 审中-公开
    水过滤系统

    公开(公告)号：US20120325342A1

    公开(公告)日：2012-12-27

    申请号：US13526727

    申请日：2012-06-19

    申请人： Scott Beatty ,  Terry Tang ,  Steven Fung

    发明人： Scott Beatty ,  Terry Tang ,  Steven Fung

    IPC分类号： E03B7/07

    CPC分类号： C02F9/005 ,  B67D2210/00005 ,  C02F1/001 ,  C02F2201/005 ,  C02F2201/006 ,  C02F2209/02 ,  C02F2209/40 ,  C02F2209/445 ,  C02F2301/043 ,  C02F2307/10 ,  F25D2323/121 ,  Y10T137/794

    摘要： A water filtration system is provided that includes a filter section, a tank section, a pump section, a housing section and a dispensing section. The filter section includes a high-pressure water filter disconnected from a source of tap water. The tank section includes an outer tank member and an inner tank member fluidly connected to the filter section. The pump section is fluidly connected to the filter section via a first conduit for pumping water at a high pressure through the filter and is fluidly connected to the outer tank member via a second conduit for drawing unfiltered water from the outer tank member. The housing section houses at least one of the filter section, the tank section and the pump section. The dispensing section is fluidly connected to the inner tank member for dispensing filtered water.

    摘要翻译： 提供了一种水过滤系统，其包括过滤部分，罐部分，泵部分，壳体部分和分配部分。 过滤器部分包括与自来水源断开的高​​压水过滤器。 罐部分包括一个外部罐件和一个与过滤器部分流体连接的内部罐件。 泵部分经由第一管道流体地连接到过滤器部分，第一管道用于通过过滤器高压泵送水，并且经由用于从外罐构件抽取未过滤的水的第二管道流体地连接到外罐构件。 壳体部分容纳过滤部分，罐部分和泵部分中的至少一个。 分配部分流体地连接到内罐构件以分配过滤的水。

    Probe composition containing a binding domain and polymer chain and methods of use
    7.
    发明授权
    Probe composition containing a binding domain and polymer chain and methods of use 失效
    含有结合域和聚合物链的探针组合物及其使用方法

    公开(公告)号：US06759202B2

    公开(公告)日：2004-07-06

    申请号：US10167337

    申请日：2002-06-10

    申请人： Paul David Grossman ,  Steven Fung ,  Steven Michael Menchen ,  Sam Lee Woo ,  Emily Susan Winn-Deen

    发明人： Paul David Grossman ,  Steven Fung ,  Steven Michael Menchen ,  Sam Lee Woo ,  Emily Susan Winn-Deen

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  Y10T436/143333 ,  C12Q2565/125 ,  C12Q2525/203 ,  C12Q2525/197 ,  C12Q2563/167 ,  C12Q2535/101 ,  C12Q2565/629 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2531/137 ,  C12Q2563/119 ,  C12Q2549/119 ,  C12Q2525/161 ,  C12Q2521/501 ,  C12Q2563/131 ,  C12Q2525/101 ,  C12Q2531/113

    摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.

    摘要翻译： 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在本发明的一个实施方案中，将多个不同序列探针对加入到靶多核苷酸中，其中每个探针对包含两个多核苷酸探针元件，该多核苷酸探针元件与目标中所选择的一个目标序列的相邻部分互补 多核苷酸。 在每个探针对中，探针元件中的一个包含非多核苷酸聚合物链，当对中的元件被连接时，赋予相关探针对的特异性移动性。 该对中的另一个元素包含可检测的报告物标记。 在探针对已被允许与靶多核苷酸杂交后，杂交的多核苷酸在有效连接靶结合探针元件的末端亚基的条件下处理，当它们的末端亚基与相邻的靶基碱基配对时。 然后将连接的探针对从目标多核苷酸中释放，并在筛分基质中电泳分离，或进行色谱分离。

    Probe composition containing a binding domain and polymer chain and methods of use
    9.
    发明授权
    Probe composition containing a binding domain and polymer chain and methods of use 失效
    含有结合域和聚合物链的探针组合物及其使用方法

    公开(公告)号：US5703222A

    公开(公告)日：1997-12-30

    申请号：US561478

    申请日：1995-11-21

    申请人： Paul David Grossman ,  Steven Fung ,  Steven Michael Menchen ,  Sam Lee Woo ,  Emily Susan Winn-Deen

    发明人： Paul David Grossman ,  Steven Fung ,  Steven Michael Menchen ,  Sam Lee Woo ,  Emily Susan Winn-Deen

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q2600/156 ,  G01N27/447 ,  Y10T436/143333

    摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

    摘要翻译： 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

    Method of DNA sequencing employing a mixed DNA-polymer chain probe
    10.
    发明授权
    Method of DNA sequencing employing a mixed DNA-polymer chain probe 失效
    使用混合DNA-聚合物链探针进行DNA测序的方法

    公开(公告)号：US5624800A

    公开(公告)日：1997-04-29

    申请号：US447174

    申请日：1995-05-19

    申请人： Paul D. Grossman ,  Steven Fung ,  Steven M. Menchen ,  Sam L. Woo ,  Emily S. Winn-Deen

    发明人： Paul D. Grossman ,  Steven Fung ,  Steven M. Menchen ,  Sam L. Woo ,  Emily S. Winn-Deen

    IPC分类号： C12N15/09 ,  B01D57/02 ,  C12Q1/68 ,  G01N27/447 ,  G01N30/88 ,  G01N33/566 ,  C12P19/34

    CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6818 ,  Y10T436/143333

    摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

    摘要翻译： 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。