公开(公告)号：US5211829A

公开(公告)日：1993-05-18

申请号：US664604

申请日：1991-03-04

申请人： Kazumichi Imai ,  Masataka Koga ,  Takehiko Kitamori ,  Tsuguo Sawada ,  Jiaqi Wu

发明人： Kazumichi Imai ,  Masataka Koga ,  Takehiko Kitamori ,  Tsuguo Sawada ,  Jiaqi Wu

IPC分类号： G01N25/00 ,  G01N21/00 ,  G01N27/447 ,  G01N29/00 ,  G01N30/62 ,  G01N30/74 ,  G01N30/76 ,  G01N35/08

CPC分类号： G01N30/76 ,  G01N27/44717

摘要： The present invention is applied to an analyzer according to which a sample to be analyzed is moved through a capillary tube in liquid chromatography or capillary-zone electrophoretic method. The capillary tube has a separation zone and a detection zone. The capillary tube in the detection zone has a section in which it can vibrate and both ends of this section are fixed.Analytes of the sample introduced into the analyzer are separated from each other in the separation zone. When each of the separated analytes is led to the area irradiated with beam in the detection zone, the capillary tube in the detection zone vibrates. This vibration phenomenon is brought about by tension fluctuation of the capillary tube caused by intermittent irradiation of the detection zone with an excitation beam and absorption of the excitation beam by the analyte. Concentration of analytes in the sample can be measured by detecting amplitude of the vibration.

摘要翻译： 本发明应用于分析器，通过液相色谱法或毛细管区电泳方法将待分析样品移动通过毛细管。 毛细管具有分离区和检测区。 检测区域中的毛细管具有能够振动的部分，并且该部分的两端固定。 在分离区中引入分析仪的样品的分析物彼此分离。 当每个分离的分析物被引导到在检测区域中用光束照射的区域时，检测区域中的毛细管管振动。 这种振动现象是由于由激发束间歇地照射检测区域而引起的毛细管的张力波动和被分析物吸收激发光束引起的。 可以通过检测振动的幅度来测量样品中分析物的浓度。

公开(公告)号：US5985121A

公开(公告)日：1999-11-16

申请号：US644860

申请日：1996-05-10

申请人： Jiaqi Wu ,  Janusz B. Pawliszyn

发明人： Jiaqi Wu ,  Janusz B. Pawliszyn

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44713 ,  G01N27/44743

摘要： An improved apparatus and method for the capillary electrophoretic separation of chemical compounds comprises carrying out on-line sample preparation by means of semipermeable membranes connected to the capillary separation column for selective introduction of ampholytes into the capillary separation column. Semipermeable membrane means may also be used on-line for preliminary desalting of the sample prior to its introduction into the separation column.

摘要翻译： 用于化学化合物毛细管电泳分离的改进的装置和方法包括通过连接到毛细管分离柱的半透膜进行在线样品制备，用于将两性电解质选择性引入毛细管分离柱。 可渗透膜装置也可以在线使用以在样品进入分离塔之前进行初步脱盐。

公开(公告)号：US20090194419A1

公开(公告)日：2009-08-06

申请号：US12358724

申请日：2009-01-23

申请人： Tiemin Huang ,  Jiaqi Wu

发明人： Tiemin Huang ,  Jiaqi Wu

IPC分类号： B01D57/02

CPC分类号： G01N27/44795 ,  C07K1/285 ,  C07K1/36 ,  G01N27/44721 ,  G01N27/44773

摘要： A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements.

摘要翻译： 提供了一种用于执行毛细管等电聚焦的方法和装置，随后通过诱导的流体动力学流动或化学动员动员聚焦区域。 分离的这两个维度与实时全通道电泳检测和自动样品注入相结合，以达到比使用已知的正交毛细管二维排列可获得的分离分辨率更高的分离分辨率。

公开(公告)号：US20080035484A1

公开(公告)日：2008-02-14

申请号：US11827098

申请日：2007-07-10

申请人： Jiaqi Wu ,  Tiemin Huang ,  Arthur Watson

发明人： Jiaqi Wu ,  Tiemin Huang ,  Arthur Watson

IPC分类号： B01D57/02 ,  B01J19/08

CPC分类号： G01N27/44795 ,  B01L3/5027 ,  G01N1/40 ,  G01N27/44739 ,  G01N2001/4038

摘要： An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.

摘要翻译： 公开了用于精确选择和提取在微通道中执行的等电聚焦产生的聚焦区域中的选定分析物的装置和方法。 交叉通道微流体装置包括在交点处彼此流体连通的样品混合物引入和分离通道和提取通道。 提供了用于选择性地将cIEF之后的分离区域的图案移动到交点的装置，并且提供用于施加提取压力以将包含所选分析物的单个区域引导到提取通道中并随后从提取通道收集的装置。

公开(公告)号：US10107782B2

公开(公告)日：2018-10-23

申请号：US12358724

申请日：2009-01-23

申请人： Tiemin Huang ,  Jiaqi Wu

发明人： Tiemin Huang ,  Jiaqi Wu

IPC分类号： G01N27/447 ,  C07K1/28 ,  C07K1/36

摘要： A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements.

公开(公告)号：US20140021053A1

公开(公告)日：2014-01-23

申请号：US13573799

申请日：2012-10-03

申请人： Jiaqi Wu ,  Tiemin Huang ,  Arthur H. Watson

发明人： Jiaqi Wu ,  Tiemin Huang ,  Arthur H. Watson

IPC分类号： G01N27/447

CPC分类号： G01N27/44795 ,  B01L3/5027 ,  G01N1/40 ,  G01N27/44739 ,  G01N2001/4038

摘要： An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.

摘要翻译： 公开了用于精确选择和提取在微通道中执行的等电聚焦产生的聚焦区域中的选定分析物的装置和方法。 交叉通道微流体装置包括在交点处彼此流体连通的样品混合物引入和分离通道和提取通道。 提供了用于选择性地将cIEF之后的分离区域的图案移动到交点的装置，并且提供用于施加提取压力以将包含所选分析物的单个区域引导到提取通道中并随后从提取通道收集的装置。

公开(公告)号：US20130289361A1

公开(公告)日：2013-10-31

申请号：US13459991

申请日：2012-04-30

申请人： Lauren Renee Bridge ,  David Momont ,  Brian Dentino ,  Jiaqi Wu

发明人： Lauren Renee Bridge ,  David Momont ,  Brian Dentino ,  Jiaqi Wu

IPC分类号： A61B5/04 ,  A61B5/0205 ,  A61B5/0245 ,  A61B5/11 ,  A61B5/0478 ,  A61B5/0408

CPC分类号： A61B5/0011 ,  A61B5/0006 ,  A61B5/0022 ,  A61B5/02411 ,  A61B5/0444 ,  A61B5/0476 ,  A61B5/11 ,  A61B2562/164 ,  G16H40/67

摘要： Systems, apparatus, and methods are disclosed for fetal monitoring. An example fetal monitoring device includes a removably adherent connector to connect the device to a wearer and conform the device to a contour of the wearer. The example device includes a sensor including epidermal electronics to detect physical signal(s) from the wearer and/or the wearer's fetus and collect data related to the signal(s). The example device includes a communication interface to wirelessly transmit the collected data to an external processing device. The external processing device is to process the collected data to generate an output indicative of a processing of the collected data according to a guideline or standard. The fetal monitoring device is to adhere to the contour of the wearer so as to not interfere with clothing or movement of the wearer and to form a close bond to receive signals from the wearer and her fetus.

摘要翻译： 公开了用于胎儿监测的系统，装置和方法。 胎儿监测装置的一个示例包括可移除地粘附的连接器，以将装置连接到佩戴者并使装置符合穿戴者的轮廓。 示例性装置包括传感器，其包括用于检测来自佩戴者和/或穿戴者的胎儿的身体信号的表皮电子装置，并收集与该信号有关的数据。 示例设备包括用于将收集的数据无线发送到外部处理设备的通信接口。 外部处理装置是处理所收集的数据，以根据指南或标准产生指示收集的数据的处理的输出。 胎儿监测装置要贴合穿戴者的轮廓，以便不干扰穿着者的衣服或运动，并形成紧密接合以接收穿戴者及其胎儿的信号。