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49 条记录 (耗时 0.022 秒)

    Method of separating activated human protein C
    2.
    依法登记的发明
    Method of separating activated human protein C 失效

    公开(公告)号：USH1148H

    公开(公告)日：1993-03-02

    申请号：US815870

    申请日：1992-01-03

    申请人： Kenji Wakabayashi ,  Yoshihiko Sumi ,  Yataro Ichikawa ,  Yoichi Sakata ,  Nobuo Aoki

    发明人： Kenji Wakabayashi ,  Yoshihiko Sumi ,  Yataro Ichikawa ,  Yoichi Sakata ,  Nobuo Aoki

    IPC分类号： C07K16/40 ,  C12N9/64

    CPC分类号： C12N9/6464 ,  C07K16/40 ,  C12Y304/21069

    摘要： A method of separating activated human protein C, which comprises bringing a mixture containing activated human protein C having a gamma-carboxyglutamic acid (Gla) domain in the presence of a calcium ion into contact with a fixed antibody comprising an insoluble carrier and fixed thereto an antibody to a complex of human protein C and a calcium ion bound to the Gla domain whereby the activated human protein C is captured by the fixed antibody in the form in which the calcium ion is bound to the Gla domain. A method is also provided for separating activated human protein C from a mixture containing human protein C and activated human protein C, which comprises bringing a first mixture containing human protein C and activated human protein C having a Gla domain into contact with a first fixed antibody comprising an insoluble carrier and fixed thereto a first antibody to human protein C but not to activated human protein C to form a second mixture substantially free from human protein C while the human protein C is captured by the first fixed antibody, and bringing the second mixture in the presence of a calcium ion into contact with a second fixed antibody comprising an insoluble carrier and fixed thereto a second antibody to a complex of human protein C and a calcium ion bound at the Gla domain, whereby the activated human protein C is captured by the second fixed antibody in the form in which the calcium ion is bound to the Gla domain.

    cDNA and genomic DNA encoding the amino acid sequence of human .alpha..sub.2 -plasmin inhibition
    3.
    发明授权
    cDNA and genomic DNA encoding the amino acid sequence of human .alpha..sub.2 -plasmin inhibition 失效
    编码人α2-纤溶酶抑制的氨基酸序列的cDNA和基因组DNA

    公开(公告)号：US5221738A

    公开(公告)日：1993-06-22

    申请号：US803896

    申请日：1991-12-09

    申请人： Yoshihiko Sumi ,  Yataro Ichikawa ,  Masami Murakami ,  Nobuo Aoki

    发明人： Yoshihiko Sumi ,  Yataro Ichikawa ,  Masami Murakami ,  Nobuo Aoki

    IPC分类号： C07K14/81 ,  C12N15/55

    CPC分类号： C07K14/8121

    摘要： cDNA encoding a human .alpha..sub.2 -plasmin inhibitor precursor protein represented by an amino acid sequence from the -39th Met to the 452nd Lys in FIG. 1 of the accompanying drawings; an amino acid sequence of a human .alpha..sub.2 -plasmin inhibitor precursor represented by an amino acid sequence from the -39th Met to the 452nd Lys in FIG. 1 of the accompanying drawings; and genomic DNA encoding a human .alpha..sub.2 -plasmin inhibitor protein which is composed of exons II, III, IV, V, VI, VII, VIII, IX and X in FIG. 4 of the accompanying drawings, said exons being bonded to one another via introns.

    摘要翻译： 编码人α2-纤溶酶原抑制物前体蛋白的cDNA，由图3中的第39位Met至第452位Lys的氨基酸序列表示。 附图1; 由图3中的第39位Met至第452位Lys的氨基酸序列表示的人α2-纤溶酶抑制剂前体的氨基酸序列。 附图1; 以及编码图2中由外显子II，III，IV，V，VI，VII，VIII，IX和X组成的人α2-纤溶酶抑制蛋白的基因组DNA。 附图4中，所述外显子通过内含子彼此结合。

    Immunological determination of free human protein S and C4bp-protein S complex
    6.
    发明授权
    Immunological determination of free human protein S and C4bp-protein S complex 失效
    游离人蛋白S和C4bp蛋白S复合物的免疫测定

    公开(公告)号：US5187067A

    公开(公告)日：1993-02-16

    申请号：US670383

    申请日：1991-03-14

    申请人： Yukiya Koike ,  Kenji Wakabayashi ,  Yoshihiko Sumi ,  Yataro Ichikawa

    发明人： Yukiya Koike ,  Kenji Wakabayashi ,  Yoshihiko Sumi ,  Yataro Ichikawa

    IPC分类号： C07K16/18 ,  C07K16/36 ,  C12N5/18

    CPC分类号： C07K16/18 ,  C07K16/36 ,  Y10S436/821 ,  Y10S436/824

    摘要： A method of immunologically determining free human protein S in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, the primary and secondary antibodies having the property of binding to different epitopes of free human protein S, and one of the primary and secondary antibodies being a monoclonal antibody having the property of not binding to a complex of the human protein S and human complement cofactor C4b-binding protein (C4bp) but specifically binding to the free human protein S. Also provided is a method of immunologically determining a complex of human protein S and human complement cofactor C4b-binding protein (C4bp) in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, one of the primary and secondary antibodies being a monoclonal antibody having the property of not binding to free human protein S and human complement cofactor C4b-binding protein (C4bp) but binding specifically to the complex, and the other being an antibody having the property of binding to the human complement cofactor C4b-binding protein (4Cbp).

    摘要翻译： 一种在测定样品中免疫测定游离人蛋白S的方法，其包括将固定在不溶性固体载体上的第一抗体与标记的第二抗体与测定样品接触，所述第一抗体和第二抗体具有结合不同表位的性质 游离人蛋白S，其中一种和第二种抗体是具有不与人类蛋白S和人补体辅因子C4b结合蛋白（C4bp）的复合物结合但与结合游离人蛋白质的特异性的单克隆抗体 还提供了在测定样品中免疫测定人蛋白S和人补体辅因子C4b结合蛋白（C4bp）的复合物的方法，其包括将固定于不溶性固体载体的第一抗体和标记的第二抗体与 测定样品，其中一种和第二种抗体是具有不结合特性的单克隆抗体 g释放人蛋白S和人补体辅因子C4b结合蛋白（C4bp），但与复合物特异性结合，另一种是具有与人补体辅因子C4b结合蛋白（4Cbp）结合的性质的抗体。

    Monoclonal antibody specific to human .alpha..sub.2 -plasmin inhibitor
    7.
    发明授权
    Monoclonal antibody specific to human .alpha..sub.2 -plasmin inhibitor 失效
    对人α2-纤溶酶抑制剂特异性的单克隆抗体

    公开(公告)号：US5534255A

    公开(公告)日：1996-07-09

    申请号：US716694

    申请日：1991-06-17

    申请人： Yoshihiko Sumi ,  Yukiya Koike ,  Yataro Ichikawa ,  Nobuhiko Yoshida ,  Nobuo Aoki

    发明人： Yoshihiko Sumi ,  Yukiya Koike ,  Yataro Ichikawa ,  Nobuhiko Yoshida ,  Nobuo Aoki

    IPC分类号： A61K38/00 ,  C07K14/81 ,  C07K16/38 ,  C12P21/08 ,  A61K39/00 ,  C07K16/00

    CPC分类号： C07K16/38 ,  C07K14/8121 ,  A61K38/00 ,  Y10S530/864 ,  Y10S530/866

    摘要： This invention provides a monoclonal antibody or its fragment specific to a human .alpha..sub.2 -plasmin inhibitor, said antibody having the function of specifically blocking that site of the human .alpha..sub.2 -plasmin inhibitor which inhibits the fibrinolytic activity of plasmin, and of suppressing said fibrinolytic activity inhibiting function of said .alpha..sub.2 -plasmin inhibitor, and also a hybridoma capable of producing the monoclonal antibody. Said monoclonal antibody or its fragment is useful for the immunological determination of a human .alpha..sub.2 -plasmin inhibitor, the separation or recovery of a human .alpha..sub.2 -plasmin inhibitor from a liquid containing the human .alpha..sub.2 -plasmin inhibitor, and the treatment of a thrombotic disease.

    摘要翻译： 本发明提供了对人α2-纤溶酶抑制剂特异性的单克隆抗体或其片段，所述抗体具有特异性阻断人α2-纤溶酶抑制剂的位点的功能，其抑制纤溶酶的纤维蛋白溶解活性，并抑制所述纤维蛋白溶解 所述α2-纤溶酶抑制剂的活性抑制功能，以及能够产生单克隆抗体的杂交瘤。 所述单克隆抗体或其片段可用于免疫测定人α2-纤溶酶抑制剂，从含有人α2-纤溶酶抑制剂的液体中分离或回收人α2-纤溶酶抑制剂，以及治疗 血栓性疾病

    Human immunoglobulin and enhancer gene fragments
    8.
    发明授权
    Human immunoglobulin and enhancer gene fragments 失效
    人类免疫球蛋白和增强子基因片段

    公开(公告)号：US5101024A

    公开(公告)日：1992-03-31

    申请号：US326417

    申请日：1989-03-21

    申请人： Akira Kudo ,  Satoshi Nakamura ,  Yoshihiko Sumi ,  Yataro Ichikawa ,  Takeshi Watanabe

    发明人： Akira Kudo ,  Satoshi Nakamura ,  Yoshihiko Sumi ,  Yataro Ichikawa ,  Takeshi Watanabe

    IPC分类号： C07K16/00 ,  C12N15/13 ,  C12N15/67 ,  C12N15/70

    CPC分类号： C12N15/67 ,  C07K16/00 ,  C12N15/70 ,  Y10S435/972 ,  Y10S530/866 ,  Y10S530/867

    摘要： The present invention provides an enhancer DNA segment, a gene fragment having (a) the enhancer DNA segment and (b) a structural gene such as human D, V and J gene, and a promoter. A hybrid DNA (1) having a phage DNA fragment from Charon 4A phage vector and human DNA fragments, a Charon 4A phage containing the hybrid DNA (1), a hybrid DNA (2) having a PBR322 fragment and human DNA fragment, a transformant of E. coli C600 strain transformed with the hybrid DNA (2), plasmid pSV2-H1G1 obtained by inserting a human DNA fragment into a vector pSV-2gpt, a transformant of E. coli MC 1000 strain transformed with the plasmid pSV2-H1G1 and a transformant of mouse myeloma cells J558L or NS-1 transformed with the plasmid pSV2-H1G1 are also provided.

    摘要翻译： 本发明提供增强子DNA片段，具有（a）增强子DNA片段和（b）结构基因如人D，V和J基因的基因片段和启动子。 具有来自Charon 4A噬菌体载体的噬菌体DNA片段和人DNA片段的杂交DNA（1），含有杂合DNA（1）的Charon 4A噬菌体，具有PBR322片段和人DNA片段的杂交DNA（2），转化体 通过将人DNA片段插入载体pSV-2gpt得到的质粒pSV2-H1G1，用质粒pSV2-H1G1转化的大肠杆菌MC1000菌株的转化体，和 还提供了用质粒pSV2-H1G1转化的小鼠骨髓瘤细胞J558L或NS-1的转化体。

    Terminal modifications of tumor necrosis factor
    9.
    发明授权
    Terminal modifications of tumor necrosis factor 失效
    末端修饰肿瘤坏死因子

    公开(公告)号：US5262309A

    公开(公告)日：1993-11-16

    申请号：US668517

    申请日：1991-03-22

    申请人： Satoshi Nakamura ,  Masami Fukuoka ,  Tsukio Masegi ,  Kazuo Kitai ,  Arata Kato ,  Yataro Ichikawa

    发明人： Satoshi Nakamura ,  Masami Fukuoka ,  Tsukio Masegi ,  Kazuo Kitai ,  Arata Kato ,  Yataro Ichikawa

    IPC分类号： A61K38/00 ,  C07K14/525 ,  C12N1/21 ,  C12P21/06 ,  C07K13/00 ,  C12N15/00

    CPC分类号： C07K14/525 ,  A61K38/00

    摘要： This invention relates to a novel physiologically active polypeptide containing modified amino acids on both terminals at the amino-terminus and the carboxy-terminus in the amino acid sequence of the human tumor neclosis factor (human TNF), a recombinant plasmid containing a DNA region encoding the polypeptide, a recombinant microorganism cell transformed with the recombinant plasmid, a method of producing the polypeptide by cultivating the microorganism cell, a pharmaceutical composition containing the polypeptide and a method of purifying and recovering the polypeptide. The polypeptide in this invention has excellent antitumor activity compared to the human TNF and can be utilized as an active ingredient of a pharmaceutical composition having antitumor activity.

    摘要翻译： PCT No.PCT / JP89 / 00966 Sec。 371日期1991年3月22日 102（e）1991年3月22日PCT申请1989年9月22日PCT公开号 出版物WO90 / 03395 日本1994年4月5日。本发明涉及在人肿瘤坏死因子（人TNF）的氨基酸序列的氨基末端和羧基末端两端含有修饰氨基酸的新的生理活性多肽， 含有编码该多肽的DNA区域的重组质粒，用重组质粒转化的重组微生物细胞，通过培养微生物细胞产生该多肽的方法，含该多肽的药物组合物以及纯化和回收该多肽的方法。 本发明的多肽与人TNF相比具有优异的抗肿瘤活性，可用作具有抗肿瘤活性的药物组合物的活性成分。