摘要:
This invention provides a monoclonal antibody or its fragment specific to a human .alpha..sub.2 -plasmin inhibitor, said antibody having the function of specifically blocking that site of the human .alpha..sub.2 -plasmin inhibitor which inhibits the fibrinolytic activity of plasmin, and of suppressing said fibrinolytic activity inhibiting function of said .alpha..sub.2 -plasmin inhibitor, and also a hybridoma capable of producing the monoclonal antibody. Said monoclonal antibody or its fragment is useful for the immunological determination of a human .alpha..sub.2 -plasmin inhibitor, the separation or recovery of a human .alpha..sub.2 -plasmin inhibitor from a liquid containing the human .alpha..sub.2 -plasmin inhibitor, and the treatment of a thrombotic disease.
摘要:
A method of immunologically determining free human protein S in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, the primary and secondary antibodies having the property of binding to different epitopes of free human protein S, and one of the primary and secondary antibodies being a monoclonal antibody having the property of not binding to a complex of the human protein S and human complement cofactor C4b-binding protein (C4bp) but specifically binding to the free human protein S. Also provided is a method of immunologically determining a complex of human protein S and human complement cofactor C4b-binding protein (C4bp) in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, one of the primary and secondary antibodies being a monoclonal antibody having the property of not binding to free human protein S and human complement cofactor C4b-binding protein (C4bp) but binding specifically to the complex, and the other being an antibody having the property of binding to the human complement cofactor C4b-binding protein (4Cbp).
摘要:
A protein having human plasmin inhibiting activity, substantially equivalent reactivity with human plasmin to a human .alpha..sub.2 -plasmin inhibitor derived from plasma, a binding ability to human fibrin about 1/4 to about 1/3 of that of the human .alpha..sub.2 -plasmin inhibitor derived from plasma, and a molecular weight of about 50,000 to about 77,000.
摘要:
A protein having human plasmin inhibiting activity, substantially equivalent reactivity with human plasmin to a human .alpha..sub.2 -plasmin inhibitor derived from plasma, a binding ability to human fibrin about 1/4 to about 1/3 of that of the human .alpha..sub.2 -plasmin inhibitor derived from plasma, and a molecular weight of about 50,000 to about 77,000.
摘要:
cDNA encoding a human .alpha..sub.2 -plasmin inhibitor precursor protein represented by an amino acid sequence from the -39th Met to the 452nd Lys in FIG. 1 of the accompanying drawings; an amino acid sequence of a human .alpha..sub.2 -plasmin inhibitor precursor represented by an amino acid sequence from the -39th Met to the 452nd Lys in FIG. 1 of the accompanying drawings; and genomic DNA encoding a human .alpha..sub.2 -plasmin inhibitor protein which is composed of exons II, III, IV, V, VI, VII, VIII, IX and X in FIG. 4 of the accompanying drawings, said exons being bonded to one another via introns.
摘要:
A method of separating activated human protein C, which comprises bringing a mixture containing activated human protein C having a gamma-carboxyglutamic acid (Gla) domain in the presence of a calcium ion into contact with a fixed antibody comprising an insoluble carrier and fixed thereto an antibody to a complex of human protein C and a calcium ion bound to the Gla domain whereby the activated human protein C is captured by the fixed antibody in the form in which the calcium ion is bound to the Gla domain. A method is also provided for separating activated human protein C from a mixture containing human protein C and activated human protein C, which comprises bringing a first mixture containing human protein C and activated human protein C having a Gla domain into contact with a first fixed antibody comprising an insoluble carrier and fixed thereto a first antibody to human protein C but not to activated human protein C to form a second mixture substantially free from human protein C while the human protein C is captured by the first fixed antibody, and bringing the second mixture in the presence of a calcium ion into contact with a second fixed antibody comprising an insoluble carrier and fixed thereto a second antibody to a complex of human protein C and a calcium ion bound at the Gla domain, whereby the activated human protein C is captured by the second fixed antibody in the form in which the calcium ion is bound to the Gla domain.
摘要:
A monoclonal antibody which is specific to human protein C which has calcium bound at the gammacarboxyglutamic acid(Gla) domain and does not recognize human protein C which is not bound to calcium at the Gla domain. The monoclonal antibody is used in an immunoassay method for determining human protein C having a Gla domain and in a method for recovering human protein C having a Gal domain.
摘要:
The present invention provides an enhancer DNA segment, a gene fragment having (a) the enhancer DNA segment and (b) a structural gene such as human D, V and J gene, and a promoter. A hybrid DNA (1) having a phage DNA fragment from Charon 4A phage vector and human DNA fragments, a Charon 4A phage containing the hybrid DNA (1), a hybrid DNA (2) having a PBR322 fragment and human DNA fragment, a transformant of E. coli C600 strain transformed with the hybrid DNA (2), plasmid pSV2-H1G1 obtained by inserting a human DNA fragment into a vector pSV-2gpt, a transformant of E. coli MC 1000 strain transformed with the plasmid pSV2-H1G1 and a transformant of mouse myeloma cells J558L or NS-1 transformed with the plasmid pSV2-H1G1 are also provided.
摘要:
A cylindrical body is produced which is composed of a fiber structure with a basis weight of 1-50 g/m2 and having a diameter of 0.5-50 mm and a bellows-shaped section, wherein the crest-to-crest spacing of the bellows-shaped section is no greater than 2 mm and the crest-to-valley depth of the bellows-shaped section is 0.01-1 mm; collagen is added to the cylindrical body to produce a composite including the cylindrical body and collagen.
摘要翻译:制造圆筒体,其由基重为1-50g / m 2且直径为0.5-50mm的纤维结构和波纹管状部分组成,其中波纹管的峰间距 形状截面不大于2mm,波纹状截面的波谷深度为0.01-1mm; 将胶原蛋白加入到圆柱体中以产生包括圆柱体和胶原蛋白的复合物。
摘要:
A process for production of a nonwoven fabric, which comprises a step wherein a thermoplastic polymer is dissolved in a mixed solvent composed of a volatile good solvent and a volatile poor solvent, a step wherein the resulting solution is spun by an electrospinning method and a step wherein a nonwoven fabric accumulated on a collecting sheet is obtained, is employed to provide a nonwoven fabric having a surface area sufficiently large as a matrix for cell culturing in the field of regenerative medicine, with large gaps between filaments and a low apparent density suitable for cell culturing.