摘要:
To provide an industrial purification method of PDC obtained by fermentative production.A method of purifying 2-pyrone-4,6-dicarboxylic acid which comprises including a salt of monovalent to tetravalent cations in a fermentation liquid containing microbially-produced 2-pyrone-4,6-dicarboxylic acid; and a method of purifying 2-pyrone-4,6-dicarboxylic acid which method comprises extracting 2-pyrone-4,6-dicarboxylic acid from a fermentation liquid containing microbially-produced 2-pyrone-4,6-dicarboxylic acid without forming 2-pyrone-4,6-dicarboxylate.
摘要:
There is provided a process for industrial production of simple 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone from low molecular mixtures derived from plant components such as vanillin, vanillic acid and protocatechuic acid, via a multistage enzyme reaction. A recombinant plasmid containing a vanillate demethylase gene (vanAB genes), benzaldehyde dehydrogenase gene (ligV gene) and protocatechuate 3,4-dioxygenase gene (pcaHG genes); transformants incorporating the plasmid; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of vanillin, vanillic acid, protocatechuic acid or a mixture of two or more thereof.
摘要:
There is provided a process for industrial production of simple 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone from low molecular mixtures derived from plant components such as vanillin, vanillic acid and protocatechuic acid, via a multistage enzyme reaction.A recombinant plasmid containing a vanillate demethylase gene (vanAB genes), benzaldehyde dehydrogenase gene (ligV gene) and protocatechuate 3,4-dioxygenase gene (pcaHG genes); transformants incorporating the plasmid; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of vanillin, vanillic acid, protocatechuic acid or a mixture of two or more thereof.
摘要:
A polyester containing a biomass-derived material in a repeating unit structure and having all of sufficient mechanical strength, easy mold processability into a molded product such as fiber or film, and biodegradability, is provided.A PDC-L-lactic acid copolyester represented by the following formula: —[R1—PDC]x—[OCH(CH3)CO]1-x— (I) [wherein PDC is a dicarboxylic acid residue represented by the following formula (II): R1 is selected from —O(CH2)nO—, —O(CH2CH2O)m— and —O[CH(CH3)CH2O]m—, where n represents an integer of 3 to 6 and m represents an integer of 1 to 4; and is 0.005≦x≦0.2 when n≦4 or m≦2, and is 0.005≦x≦0.4 in other cases]; and a molded product containing the PDC-L-lactic acid copolyester.
摘要:
The invention provides biodegradable polyesters, polyurethanes, comprising 3-carboxymuconolactone as a structural unit, 3-carboxymuconolactone esters and a process for their production.Polyesters comprising a repeating unit represented by the following formula (I): [wherein R represents a hydrocarbon-based divalent residue which, in the structure, optionally contains a heteroatom without an active hydrogen], polyurethanes comprising the polyester structure; 3-carboxymuconolactone esters; and a process for their production.
摘要:
Industrial-scale fermentative production of 3-carboxy-cis,cis-muconic acid from terephthalic acid. Also, a protocatechuate 4,5-ring-cleaving enzyme gene-disrupted strain in which the gene coding for (a) the amino acid sequence set forth in SEQ ID NO: 1 or 3, or (b) the amino acid sequence set forth in SEQ ID NO: 1 or 3 which has a deletion, substitution, addition and/or insertion of one or more amino acids and exhibits protocatechuate 4,5-ring cleavage activity, present in the chromosomal DNA of microbial cells, has been disrupted; recombinant plasmids comprising the Tph gene and protocatechuate 3,4-dioxygenase gene; transformants obtained by introducing the recombinant plasmids into the disrupted strain; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of terephthalic acid.
摘要翻译:从对苯二甲酸工业规模发酵生产3-羧基 - 顺式,顺式 - 粘康酸。 此外,原儿茶素4,5环切割酶基因破坏的菌株,其中编码(a)SEQ ID NO:1或3所示的氨基酸序列的基因,或(b)阐述的氨基酸序列 在具有一个或多个氨基酸的缺失,取代,添加和/或插入并且表现出存在于微生物细胞的染色体DNA中的原儿茶素4,5-环切割活性的SEQ ID NO:1或3已被破坏; 包含Tph基因和原儿茶素3,4-双加氧酶基因的重组质粒; 通过将重组质粒导入破碎的菌株获得的转化体; 以及3-羧基 - 顺式,顺式 - 粘酸和/或3-羧基粘土内酯的制备方法,其特征在于在对苯二甲酸存在下培养转化体。
摘要:
A high-elasticity biodegradable polymer comprising PDC, which has excellent solubility in solvents and reactants, in their repeating unit structures is provided in an inexpensive manner.A polymer comprising a repeating unit represented by the following formula (I): wherein R1 and R2 each independently represent a divalent hydrocarbon residue with no active hydrogens in its structure and optionally containing a heteroatom; X represents O or NH; x represents an integer of at least 1; and m and n represent 0 or 1; and a process for their production.
摘要:
In a lithium-ion secondary battery using a positive-electrode active material that includes a lithium-manganese-based oxide which includes a lithium (Li) element and a tetravalent manganese (Mn) element and whose crystal structure belongs to a layered rock-salt structure, adding a compound being selected from the group consisting of Compounds (a) through (i) into the electrolytic solution leads to the following: degradations due to oxidation-reduction decompositions of the electrolytic solution, and so on, are inhibited; and not only the shelf or storage capacity and recovered capacity upgrade in the case of being stored at high temperatures, but also the rise of internal resistance is inhibited.
摘要:
Soy milk having a concentration of 10% to 15% is mixed with coagulant agent liquid that is obtained by dissolving a coagulant agent in warm water that is in an amount of 0.5 to 1.5 times a volume of the soy milk to obtain a curd. The coagulant agent coagulates protein contained in the soy milk. Thus obtained curd is dehydrated so that its water content to mass of the whole curd is within a range from 60% to 72% to obtain a processed soybean food of the present invention. In the present invention, the coagulant agent contains a compound selected from a group consisting of magnesium chloride and calcium chloride in a ratio of 0.2% to 0.5% of mass of the soy milk.