摘要:
A compound microscope device allowing simultaneous observation of one specimen by a transmission electron microscope and an optical microscope, is provided. A compound microscope device 1 of the present invention has a transmission electron microscope 2 and an optical microscope 4. A specimen 10 and a reflection mirror 41 are disposed on an electron optical axis C of an electron ray. The reflection mirror 41 is inclined from the electron optical axis C toward the optical object lens 43 and the specimen 10. Light from the specimen 10 (fluorescent light, reflection light, and the like) is reflected by the reflection mirror 41 and entered into the optical object lens 43. The electron ray from the electron microscope 2 passes through a mounting center hole 42 of the reflection mirror 41. This makes it possible to observe one specimen simultaneously by the electron microscope 2 and the optical microscope 4.
摘要:
A compound microscope device allows simultaneous observation of one specimen by a transmission electron microscope and an optical microscope. The compound microscope device 1 of the present invention has a transmission electron microscope 2 and an optical microscope 4. A specimen 10 and a reflection mirror 41 are disposed on an electron optical axis C of an electron ray. The reflection mirror 41 is inclined from the electron optical axis C toward the optical object lens 43 and the specimen 10. Light from the specimen 10 (fluorescent light, reflection light, and the like) is reflected by the reflection mirror 41 and enters into the optical object lens 43. The electron ray from the electron microscope 2 passes through a mounting center hole 42 of the reflection mirror 41. This makes it possible to observe one specimen simultaneously by the electron microscope 2 and the optical microscope 4.
摘要:
A method of printing an image of a three-dimensional object includes printing multiple dots of a colored ink to form an image of the object and printing dots of a transparent ink having a refractive index greater than 1 within the image. The dots of transparent ink local alter the path length of light that shines through the image to create phase variations on the image.
摘要:
A phase plate (10) for phase-contrast electron microscopes is characterized by comprising a conductive core phase plate (14) which has a phase plate body (11) and a phase plate support (12) supporting it and is arranged in the path of electrons having passed through the objective lens of an electron microscope and in which the phase plate body (11) is so supported on the phase plate support (12) having an opening (13) as to cover at least a part of the opening (13) and a conductive shield thin film (15) covering the periphery of the core phase plate (14) including the upper and lower sides thereof. Consequently, a phase plane for phase-contrast electron microscopes preventing the lens effect incident to charging completely and applicable to the field of material science, its manufacturing method and a phase-contrast electron microscope can be provided.
摘要:
There is disclosed a method of symmetrizing a three-dimensional NMR spectrum which is obtained by applying a preparation pulse or pulse sequence, sampling the resulting free induction decay signals with a sequence having two evolution periods and a detection period to obtain data S (t.sub.1, t.sub.2, t.sub.3), and taking the Fourier transform of the data to create the three-dimensional NMR spectrum .alpha. (.omega..sub.1, .omega..sub.2, .omega..sub.3). The 3D NMR spectrum is sliced perpendicularly to the frequency axis .omega..sub.3. Data about the 3D NMR spectrum is arithmetically processed in such a way that peaks having no symmetrical partners at symmertical positions determined also taking account of the direction of the frequency axis .omega..sub.3 are found and erased to obtain a spectrum .alpha..sub.1 (.omega..sub.1, .omega..sub.2, .omega..sub.3). The data about this spectrum is arithmetically processed in such a way that peaks complementary to the peaks having no symmetrical partners in the sliced planes of the spectrum .alpha..sub.1 (.omega..sub.1, .omega..sub.2, .omega..sub.3 ) are added to obtain a symmetrized spectrum .alpha..sub.2 (.omega..sub.1, .omega..sub.2, .omega..sub.3).
摘要:
A method of printing an image of a three-dimensional object includes printing multiple dots of a colored ink to form an image of the object and printing dots of a transparent ink having a refractive index greater than 1 within the image. The dots of transparent ink local alter the path length of light that shines through the image to create phase variations on the image.
摘要:
A method for modifying nucleic acid bases by a chemical means, which enables the discrimination of every base species in plural species of bases in a nucleic acid comprising plural nucleotide units, while retaining the base sequence information of the nucleic acid. A nucleic acid base-modified product provided by the method. The nucleic acid base-modified product is essentially a single strand. In accordance with the invention, a novel means for sequencing a nucleic acid by a microscopic means is provided.
摘要:
Novel DNA sequence determination method and DNA sequencer system providing a sequencing speed 103 to 104 times faster than the current DNA sequencing speed (105 bases per day with a lane at maximum) of the existing DNA sequencer based on electrophoresis. The method includes the step of discriminating base-specific labels of heavy elements using a magnified image of elongated single-chain DNA or RNA produced by a transmission electron microscope (TEM). The DNA sequencer system uses this method. The invention provides a DNA sequencing speed that is higher than the existing speed by 3 or 4 orders of magnitude.
摘要:
A method for modifying nucleic acid bases by a chemical means, which enables the discrimination of every base species in plural species of bases in a nucleic acid comprising plural nucleotide units, while retaining the base sequence information of the nucleic acid. A nucleic acid base-modified product provided by the method. The nucleic acid base-modified product is essentially a single strand. In accordance with the invention, a novel means for sequencing a nucleic acid by a microscopic means is provided.
摘要:
A phase plate for an electron microscope in which a portion of a magnetic thin-wire ring or a magnetic thin-wire rod (15) spans an opening of a support member (14) having the opening, the magnetic thin-wire ring or magnetic thin-wire rod (15) generates a vector potential, and a phase difference is formed between electron beams that pass through left and right sides of a spanning portion of the magnetic thin-wire ring or the magnetic thin-wire rod (15). The phase plate prevents the electron beam loss more effectively, can be applied at an accelerating voltage within a wide rage from a low voltage to a high voltage, causes no difficulties in production, has good utility, and makes it possible to obtain a high-contrast image.