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公开(公告)号：US08642256B2
公开(公告)日：2014-02-04
申请号：US12508513
申请日：2009-07-23
申请人： Kurt Vogel , Steven Riddle , Robert Horton , Matthew Robers , Gregory Michaud , Thomas Machleidt , Kevin Vedvik , Kristin Huwiler
发明人： Kurt Vogel , Steven Riddle , Robert Horton , Matthew Robers , Gregory Michaud , Thomas Machleidt , Kevin Vedvik , Kristin Huwiler
IPC分类号： C12Q1/00
CPC分类号： G01N33/542 , C12Q1/37
摘要： Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing molecular interactions (e.g., deubiquination, ubiquination and kinase activity), e.g., using resonance energy transfer (RET) are provided.
摘要翻译： 提供了组合物，包括抗体，多肽和有机分子，试剂盒和用于探测分子相互作用（例如，去泛素化，泛素化和激酶活性）的方法，例如使用共振能量转移（RET）。
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公开(公告)号：US20080138836A1
公开(公告)日：2008-06-12
申请号：US11781124
申请日：2007-07-20
申请人： Gregory Michaud , Kurt Vogel , Matthew Robers , Robert Horton , Steven Riddle
发明人： Gregory Michaud , Kurt Vogel , Matthew Robers , Robert Horton , Steven Riddle
IPC分类号： G01N33/573 , G01N33/53
摘要： Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing molecular interactions (e.g., deubiquination, ubiquination and kinase activity) using resonance energy transfer (RET) are provided.
摘要翻译： 提供了使用共振能量转移（RET）的组合物，包括抗体，多肽和有机分子，试剂盒和用于探测分子相互作用（例如，去泛素化，泛素化和激酶活性）的方法。
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公开(公告)号：US20080213811A1
公开(公告)日：2008-09-04
申请号：US11837180
申请日：2007-08-10
申请人： Kurt Vogel , Rhonda Newman , Steven Riddle
发明人： Kurt Vogel , Rhonda Newman , Steven Riddle
CPC分类号： G01N33/542 , G01N33/84 , Y10T436/16
摘要： The present invention relates to biosensors. In some embodiments, the biosensors are modified ligand binding molecules. In some embodiments, the modified ligand binding molecule is a phosphate binding protein (PBP). In some embodiments, the modified ligand binding molecules are labeled to be capable of RET, e.g., comprising a donor and acceptor moiety. In some embodiments of the invention, there is a detectable change in RET (e.g., FRET) when the modified ligand binding molecule binds and/or releases the ligand (e.g., phosphate). The invention also provides related methods, reactions and assays.
摘要翻译： 本发明涉及生物传感器。 在一些实施方案中，生物传感器是修饰的配体结合分子。 在一些实施方案中，修饰的配体结合分子是磷酸结合蛋白（PBP）。 在一些实施方案中，修饰的配体结合分子被标记为能够RET，例如包含供体和受体部分。 在本发明的一些实施方案中，当修饰的配体结合分子结合和/或释放配体（例如磷酸盐）时，在RET（例如，FRET）中存在可检测的变化。 本发明还提供相关方法，反应和测定。
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公开(公告)号：US08409814B2
公开(公告)日：2013-04-02
申请号：US12874140
申请日：2010-09-01
申请人： Kurt Vogel , Rhonda Newman , Steven Riddle
发明人： Kurt Vogel , Rhonda Newman , Steven Riddle
CPC分类号： G01N33/542 , G01N33/84 , Y10T436/16
摘要： The present invention relates to biosensors. In some embodiments, the biosensors are modified ligand binding molecules. In some embodiments, the modified ligand binding molecule is a phosphate binding protein (PBP). In some embodiments, the modified ligand binding molecules are labeled to be capable of RET, e.g., comprising a donor and acceptor moiety. In some embodiments of the invention, there is a detectable change in RET (e.g., FRET) when the modified ligand binding molecule binds and/or releases the ligand (e.g., phosphate). The invention also provides related methods, reactions and assays.
摘要翻译： 本发明涉及生物传感器。 在一些实施方案中，生物传感器是修饰的配体结合分子。 在一些实施方案中，修饰的配体结合分子是磷酸结合蛋白（PBP）。 在一些实施方案中，修饰的配体结合分子被标记为能够RET，例如包含供体和受体部分。 在本发明的一些实施方案中，当修饰的配体结合分子结合和/或释放配体（例如磷酸盐）时，在RET（例如，FRET）中存在可检测的变化。 本发明还提供相关方法，反应和测定。
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公开(公告)号：US07619059B2
公开(公告)日：2009-11-17
申请号：US10937042
申请日：2004-09-09
申请人： Kevin R. Kupcho , Kurt Vogel , Elizabeth A. Werner , Jane A. Beebe , Tony A. Klink , David A. Lasky , Karen M. Kleman-Leyer , Richard Somberg
发明人： Kevin R. Kupcho , Kurt Vogel , Elizabeth A. Werner , Jane A. Beebe , Tony A. Klink , David A. Lasky , Karen M. Kleman-Leyer , Richard Somberg
CPC分类号： C12Q1/42 , C12Q1/485 , G01N2440/14
摘要： Compositions, methods, and kits for detecting and monitoring post-translational modification activities, including kinase or phosphatase activities, are described. The compositions typically include a peptide, a first detectable moiety, a first binding member, and a protease cleavage site. Modification of a composition by a post-translational modification enzyme, such as a kinase or phosphatase, alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition when it is associated noncovalently with a probe composition that includes a second binding member and a second detectable moiety. Panel assays for determining substrates or modulators of enzymatic activities are also described.
摘要翻译： 描述了用于检测和监测翻译后修饰活性（包括激酶或磷酸酶活性）的组合物，方法和试剂盒。 组合物通常包括肽，第一可检测部分，第一结合成员和蛋白酶切割位点。 通过翻译后修饰酶（例如激酶或磷酸酶）修饰组合物改变了肽的蛋白水解敏感性，导致当组合物非共价地与探针组合物相关联时，组合物的可检测性质发生变化，所述探针组合物包括 第二结合成员和第二可检测部分。 还描述了用于确定酶活性的底物或调节剂的面板测定。
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6.发明申请Administering bifunctional molecules containing a drug moiety and presenter protein ligand for therapy 有权管理含有药物部分的双功能分子和用于治疗的展示蛋白配体公开(公告)号：US20050209146A1
公开(公告)日：2005-09-22
申请号：US11011499
申请日：2004-12-13
申请人： Roger Briesewitz , Gerald Crabtree , Thomas Wandless , Gregory Ray , Kurt Vogel
发明人： Roger Briesewitz , Gerald Crabtree , Thomas Wandless , Gregory Ray , Kurt Vogel
CPC分类号： C12N9/90 , A61K38/00 , A61K47/54 , A61K47/552 , C07K5/1027 , C07K7/06 , C07K2319/00 , Y10S530/812
摘要： Bifunctional molecules and methods for their use in the production of binary complexes in a host are provided. The bifunctional molecule is a conjugate of a drug moiety and a presenter protein ligand. In the subject methods, an effective amount of the bifunctional molecule is administered to the host. The bifunctional molecule binds to the presenter protein to produce a binary complex that exhibits at least one of improved affinity, specificity or selectivity as compared to the corresponding free drug. The subject methods and compositions find use in a variety of therapeutic applications.
摘要翻译： 提供了双功能分子及其在宿主生产二元配合物中的应用。 双功能分子是药物部分和呈递蛋白质配体的缀合物。 在本方法中，向宿主施用有效量的双功能分子。 与相应的游离药物相比，双功能分子与展示蛋白结合以产生显示出改善的亲和力，特异性或选择性中的至少一种的二元复合物。 主题方法和组合物可用于各种治疗应用。
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公开(公告)号：US20120231541A1
公开(公告)日：2012-09-13
申请号：US13417008
申请日：2012-03-09
申请人： David Piper , Stephen Hess , Mohammed Saleh Shekhani , Thomas Livelli , Zhong Zhong , Steve Duff , Kurt Vogel
发明人： David Piper , Stephen Hess , Mohammed Saleh Shekhani , Thomas Livelli , Zhong Zhong , Steve Duff , Kurt Vogel
CPC分类号： G01N33/582 , C07D491/107 , G01N33/6872 , Y10T436/13
摘要： Disclosed are assays, methods, and kits for the screening of test compounds for their capability to induce cardiotoxicity in a subject. In particular, whether a test compound has the effect to prolong the Q-T interval as measured by an electrocardiogram in a human. The assays, methods, and kits disclosed herein make use of the binding interaction between novel fluorescent tracers and the hERG K+ channel, and the propensity of a test compound to influence that binding interaction.
摘要翻译： 公开了用于筛选测试化合物在受试者中诱导心脏毒性的能力的测定，方法和试剂盒。 特别地，测试化合物是否具有延长通过人心电图测量的Q-T间期的作用。 本文公开的测定法，方法和试剂盒利用新型荧光示踪剂和hERG K +通道之间的结合相互作用以及测试化合物影响该结合相互作用的倾向。
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公开(公告)号：US20080220118A1
公开(公告)日：2008-09-11
申请号：US11942242
申请日：2007-11-19
申请人： Allan Svendsen , Soren Flensted Lassen , Dirk Kostrewa , Luis Pasamontes , Martin Lehman , Andrea Tomschy , Adolphus Van Loon , Kurt Vogel , Markus Wyss
发明人： Allan Svendsen , Soren Flensted Lassen , Dirk Kostrewa , Luis Pasamontes , Martin Lehman , Andrea Tomschy , Adolphus Van Loon , Kurt Vogel , Markus Wyss
CPC分类号： C12N15/8257 , A23K10/14 , A23K20/189 , C12N9/16 , C12N15/8242 , C12N15/8243 , Y10S435/911 , Y10S435/916
摘要： The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1, are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.
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公开(公告)号：US07407786B2
公开(公告)日：2008-08-05
申请号：US10920059
申请日：2004-08-17
申请人： Lorraine J. Giver , Jeremy Minshull , Kurt Vogel
发明人： Lorraine J. Giver , Jeremy Minshull , Kurt Vogel
CPC分类号： C12N9/20 , A61K38/00 , C12N15/1034
摘要： New lipase enzymes (both nucleic acids and polypeptides) are provided. Compositions which include these polypeptides, proteins, nucleic acids, recombinant cells, as well as methods involving the enzymes, antibodies to the enzymes, and methods of using the enzymes are also provided.
摘要翻译： 提供新的脂肪酶（核酸和多肽）。 还提供了包括这些多肽，蛋白质，核酸，重组细胞以及涉及酶的方法，酶的抗体以及使用酶的方法的组合物。
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公开(公告)号：US08133695B2
公开(公告)日：2012-03-13
申请号：US12394605
申请日：2009-02-27
申请人： David Piper , Kurt Vogel , Mohammed Saleh Shekhani , Stephen Hess , Steve Duff , Thomas Livelli , Zhong Zhong
发明人： David Piper , Kurt Vogel , Mohammed Saleh Shekhani , Stephen Hess , Steve Duff , Thomas Livelli , Zhong Zhong
IPC分类号： G01N33/53 , G01N33/532 , G01N33/533
CPC分类号： G01N33/582 , C07D491/107 , G01N33/6872 , Y10T436/13
摘要： Disclosed are assays, methods, and kits for the screening of test compounds for their capability to induce cardiotoxicity in a subject. In particular, whether a test compound has the effect to prolong the Q-T interval as measured by an electrocardiogram in a human. The assays, methods, and kits disclosed herein make use of the binding interaction between novel fluorescent tracers and the hERG K+ channel, and the propensity of a test compound to influence that binding interaction.
摘要翻译： 公开了用于筛选测试化合物在受试者中诱导心脏毒性的能力的测定，方法和试剂盒。 特别地，测试化合物是否具有延长通过人心电图测量的Q-T间期的作用。 本文公开的测定法，方法和试剂盒利用新型荧光示踪剂和hERG K +通道之间的结合相互作用以及测试化合物影响该结合相互作用的倾向。
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