Abstract:
The present inventors discovered a novel genome coding Phytolacca insularis antiviral protein(PIP) isolated from Phytolacca insularis Nakai; and developed a recombinant vector for said PIP genome expression and a microorganism transformed therewith. PIP genome of the present invention has nucleotide homology of about 82%, compared with the genome of Phytolacca americana antiviral protein isolated from Phytolacca americana L., which is closely related to the Phytolacca insularis Nakai. PIP cDNA is consist of 918 bp of one open reading frame and termination codon; and polyadenylation signal which is ubiquitous in mRNA of most plants and animals, appears to be located in the upstream of 33 bp from the polyadenylation site. Recombinant PIP of the invention was proved to inhibit the growth of E. coli HB101 transformed with said expression vector.
Abstract:
The present invention provides a recombinant vector for phytolacca antiviral protein isolated from Phytolacca americana L. and a process for preparing transgenic plants transformed therewith. The transgenic plants of the invention were properly transformed with the recombinant vector; PAP was expressed from the transgenic plants in a successful manner; and, virus proliferation was efficiently inhibited by the expressed PAP in the transgenic plants.
Abstract translation:本发明提供了从美国植物医药公司(Phytolacca americana L.)分离的植物抗病毒蛋白质的重组载体,以及用于转化的转基因植物的制备方法。 用重组载体适当转化本发明的转基因植物; 以转基因植物成功表达PAP; 并且转基因植物中表达的PAP有效地抑制病毒增殖。
Abstract:
The present inventors discovered a novel genome coding Phytolacca insularis antiviral protein(PIP) isolated from Phytolacca insularis Nakai; and developed a recombinant vector for said PIP genome expression and a microorganism transformed therewith. PIP genome of the present invention has nucleotide hornology of about 82%, compared with the genome of Phytolacca americana antiviral protein isolated from Phytolacca americana L., which is closely related to the Phytolacca insularis Nakai. PIP cDNA is consist of 918 bp of one open reading frame and termination codon; and polyaclenylation signal which is ubiquitous in mRNA of most plants and animals, appears to be located in the upstream of 33 bp from the polyadenylation site. Recombinant PIP of the invention was proved to inhibit the growth of E. coli HB101 transformed with said expression vector.
Abstract:
An insulation structure of a cargo tank in an LNG carrier and a method of constructing the insulation structure include a first metal foil attached and installed in between the top insulation panel and the bottom insulation panel, a second metal foil attached to and installed on the first metal foil that is positioned on an upper side of a gap formed between the bottom insulation panels, and a top bridge panel attached to and installed on an upper side of the second metal foil.
Abstract:
A heater cover for microwave ovens using halogen heaters in addition to magnetrons is disclosed. The above heater cover is designed to have an optimal opening ratio while effectively protecting the halogen heaters from microwaves and having a desired structural strength. On the heater cover, a plurality of perforations are formed along a plurality of rows with both the same interval between the perforations and a radius "r" of each perforation being three times or more of the interval. The interval between horizontal phantom lines, passing through the centers of the perforations arranged on the rows, is shorter than the diameter "2r" of each perforation. The relation between the radius "r" of each perforation and the wavelength ".lambda." of microwaves is expressed by the expression, .lambda./64 .ltoreq.2r .ltoreq..lambda./8. The interval between the perforations ranges from 0.5 mm to 2 mm.
Abstract:
A peripheral device in need of power supply connected to a parallel port of an expansion device linking the portable computer system, and to a parallel port of the portable computer system, which will be supplied power through the parallel port. In this computer system, the first parallel port must be installed and the second parallel port must be extended from the portable computer to the expansion device. And between the first and second parallel port, the first and the second peripheral device interconnection recognizing mechanism must be equipped to recognize the type of peripheral device. Also the first and the second power supply must be equipped to cut off or on the power to the peripheral device through the parallel port when the first and the second peripheral device interconnection recognizing mechanism give the recognizing signal. Furthermore the first power supply and the second power supply mechanism doesn't supply power when it recognizes that no peripheral device is connected to the first and second parallel port, or a peripheral device with no need of the power supply is connected. The first and the second power supply mechanism supplies power to a peripheral device through the first and second parallel port when it recognizes that a peripheral device in need of power supply is connected to the first parallel port and the second parallel port.
Abstract:
The present invention provides a novel expression vector for Phytolacca antiviral protein isolated from Phytolacca americana L. and a microorganism transformed with said PAP expression vector. In accordance with the present invention, biologically active PAP can be produced in a massive manner from the microorganism transformed with the expression vector of the invention; and, therefore, the recombinant PAP can be practically applied to various fields, e.g., the molecular studies on PAP and treatment of AIDS employing immunoconjugate.
Abstract translation:本发明提供了从美国植物医药(Phytolacca americana L.)分离的植物疫苗抗病毒蛋白质的新型表达载体和用所述PAP表达载体转化的微生物。 根据本发明,可以从用本发明的表达载体转化的微生物中大规模生产生物活性的PAP; 因此,重组PAP可以实际应用于各种领域,例如对PAP的分子研究和使用免疫偶联物治疗AIDS。
Abstract:
An insulation structure of a cargo tank in an LNG carrier and a method of constructing the insulation structure include a first metal foil attached and installed in between the top insulation panel and the bottom insulation panel, a second metal foil attached to and installed on the first metal foil that is positioned on an upper side of a gap formed between the bottom insulation panels, and a top bridge panel attached to and installed on an upper side of the second metal foil.
Abstract:
A fixing apparatus for fixing a cargo insulation panel to a hull is provided. The fixing apparatus includes a fixing plate adhered to one face of the insulation panel, a plurality of fixing brackets protruded to the outside of the fixing plate, protruded portions of the fixing brackets being fixed by stud bolts arranged on the hull, and a coupling device configured to couple the fixing brackets to the fixing plate.
Abstract:
The present invention is related to a reinforcing member for a membrane for improving the pressure-withstanding property of the membrane having corrugations, and a membrane assembly having the reinforcing member and a method of constructing the membrane assembly. By providing a reinforcing member for a membrane having corrugations and installed in an insulating structural member of an LNG cargo, the present invention can prevent the collapse of the corrugation and attenuate shocks against a same load without increasing the facial rigidity of the corrugation, and improve the insulating property by forming an additional insulating layer.