摘要:
The present invention is directed to isolated polynucleotides coding for phosphoglucose isomerase (pgi) from coryneform bacteria. In addition, the invention includes methods for increasing the metabolic flux through pentose phosphate cycle of bacteria by reducing or eliminating the activity of pgi. These methods may be used to increase the fermentative production of nucleotides, vitamins and amino acids.
摘要:
The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.
摘要:
The invention relates to an isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequences of SEQ ID NO. 2 or SEQ ID NO. 4, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequences of SEQ ID NO. 2 or SEQ ID NO. 4 c) polynucleotide which is complementary to the polynucleotides of a) or b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c) and a process for the preparation of L-amino acids, which comprises carrying out the following steps: a) fermentation of the desired L-amino acid-producing bacteria in which at least the tal gene is amplified, b) concentration of the desired product in the medium or in the cells of the bacteria and c) isolation of the L-amino acid.
摘要翻译:本发明涉及来自棒状细菌的分离的多核苷酸,其包含选自以下的多核苷酸序列:a)与编码多肽的多核苷酸的程度相同的多核苷酸,所述多核苷酸包含氨基酸序列 SEQ ID NO: 2或SEQ ID NO。 4,b)编码多肽的多核苷酸,其包含与SEQ ID NO:1的氨基酸序列至少70%的程度相同的氨基酸序列。 2或SEQ ID NO。 4)与a)或b)的多核苷酸互补的多核苷酸和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及制备L-氨基酸的方法 其包括进行以下步骤:a)发酵所需的L-氨基酸生产细菌,其中至少有tal基因被扩增,b)所需产物在培养基中或细菌中的浓度,以及 c)分离L-氨基酸。
摘要:
The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.
摘要:
The invention is directed to DNA sequences from coryneform bacteria which encode a protein having transaldolase enzymatic activity. The invention also encompasses methods for the fermentative production of L-amino acids using bacteria in which the gene encoding transaldolase is amplified.
摘要:
This application discloses methods for the preparation of L-amino acids, which comprises fermentation of a desired L-amino acid-producing bacteria in which at least the tal gene is amplified. In some embodiments, genes of the biosynthesis pathway of the desired L-amino acid are additionally amplified.
摘要:
The invention is directed to DNA sequences from coryneform bacteria, particularly Corynebacterium glutamicum, which encode a protein having transaldolase enzymatic activity. The invention also encompasses processes for the fermentative production of L-amino acids using bacteria in which a gene encoding transaldolase is amplified.
摘要:
The present invention relates to polynucleotides that encode proteins having OpcA enzymatic activity. These polynucleotides can be used for increasing lysine biosynthesis in Coryneform glutamicum.
摘要:
The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及分离的多核苷酸,其包含多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70% b)编码多肽的多核苷酸,其包含与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列,c)与a)的多核苷酸互补的多核苷酸, 或b),和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少有citA基因 以减毒形式存在,并且使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the cstA gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in attenuated form and a vector which carries at least the cstA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码cstA基因的多核苷酸序列的分离的多核苷酸,以及具有cstA基因以减毒形式存在的棒状细胞宿主细菌的宿主载体系和至少携带cstA基因的载体 至SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。