公开(公告)号：US20230129295A1

公开(公告)日：2023-04-27

申请号：US18145688

申请日：2022-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John DONOHUE ,  Daniel BEACHAM

IPC: G01N27/414 ,  C09D189/00 ,  G01N27/28 ,  G01N27/30 ,  G01N27/407

Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

公开(公告)号：US20240310319A1

公开(公告)日：2024-09-19

申请号：US18671069

申请日：2024-05-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John DONOHUE ,  Sungjoon KIM

IPC: G01N27/414 ,  C12Q1/6869

CPC classification number: G01N27/414 ,  C12Q1/6869

Abstract: A manifestation of fluid potential noise is a periodic profile characterized by a periodic saw-tooth spike in a graph of average voltage across all columns of a sensor array as a function of time. This periodic profile is indicative of an adverse impact on the function of active sensor pixels that are proximal to reference pixels in sensor devices that include a large array of chemically-sensitive field effect transistor (ChemFET) sensors. Systems, devices, and methods are described that can mitigate the impact of reference pixels on fluid potential noise.

公开(公告)号：US20200041444A1

公开(公告)日：2020-02-06

申请号：US16362367

申请日：2019-03-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew WONG ,  Todd REARICK ,  John DONOHUE

IPC: G01N27/414 ,  G16B30/00

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：US20170030854A1

公开(公告)日：2017-02-02

申请号：US15222492

申请日：2016-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Creighton BUIE ,  John DONOHUE ,  Chiu Tai Andrew WONG ,  James A. BALL ,  Wolfgang HINZ ,  James BUSTILLO ,  Jordan OWENS ,  Shifeng LI

IPC: G01N27/327 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  G01N27/3276

Abstract: Provided herein is a sensor comprising a substrate having a first reaction region and a second reaction region, a first electrode associated with the first reaction region, a second electrode associated with the second reaction region and a third electrode wherein the third electrode is common to both the first reaction region and the second reaction region.

Abstract translation: 本文提供了一种传感器，其包括具有第一反应区域和第二反应区域的基底，与第一反应区域相关联的第一电极，与第二反应区域相关联的第二电极和第三电极，其中第三电极是共同的 第一反应区和第二反应区。

公开(公告)号：US20230324368A1

公开(公告)日：2023-10-12

申请号：US18335878

申请日：2023-06-15

Applicant: Life Technologies Corporation

Inventor： Daniel BEACHAM ,  Wolfgang HINZ ,  John DONOHUE ,  Scott PARKER

IPC: G01N33/50 ,  B01L3/00 ,  G01N15/10

CPC classification number: G01N33/5038 ,  G01N33/5044 ,  B01L3/502715 ,  G01N15/1031 ,  B01L2300/0645 ,  B01L2300/0636 ,  B01L2200/16 ,  G01N2015/0065

Abstract: Systems, devices and methods for cell analysis provide an end user with real-time cell analysis and imaging of single cells in a population. Various cell analysis systems can provide both optical imaging, as well as electroscopic imaging, which is an image of cellular response as detected by sensors covering a cell footprint or cellular efflux. An automated fluidic system can provide an end-user selected sequence of reagents to cells, while precision controlled sensor array device thermostatting, and analysis compartment environmental control provide consistency in the cell analysis system environment.

公开(公告)号：US20230125382A1

公开(公告)日：2023-04-27

申请号：US18050152

申请日：2022-10-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott PARKER ,  Hannah DEVYLDERE ,  John DONOHUE ,  Daisy CHILIN

IPC: G01N27/414 ,  G06T7/10 ,  G06T5/00

Abstract: Analyzing cells disposed on a sensor array surface of a ChemFET sensor array, may include flowing a solution having a step change in pH across the sensor array surface, wherein ChemFET sensors of the sensor array generate signals in response to the step change in pH to produce electroscopic image data. Multiple frames of the electroscopic image data are acquired during an acquisition time interval. Each frame corresponds to signal samples generated by the sensor array measured at a sampling time during the acquisition time interval. Each frame comprises pixels, wherein a given pixel in the frame corresponds to a signal sample from a given sensor in the sensor array. The electroscopic image data is segmented, based on characteristics of the signal samples, into cell regions corresponding to locations of the cells on the sensor array surface and background regions corresponding to areas on the sensor array having no cells.

公开(公告)号：US20220146450A1

公开(公告)日：2022-05-12

申请号：US17499676

申请日：2021-10-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew WONG ,  Todd REARICK ,  John DONOHUE

IPC: G01N27/414 ,  G16B30/00

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：US20190204293A1

公开(公告)日：2019-07-04

申请号：US16229272

申请日：2018-12-21

Applicant: Life Technologies Corporation

Inventor： John DONOHUE ,  Phil WAGGONER ,  Scott PARKER ,  Wolfgang HINZ ,  Chiu Tai Andrew WONG

IPC: G01N33/487 ,  G01N27/02 ,  B01L3/00

CPC classification number: G01N33/48721 ,  B01L3/5085 ,  B01L2300/0645 ,  G01N27/021 ,  G01N27/028 ,  H03K17/6871

Abstract: A chemical sensor for analyte solutions utilizes AC excitation of a sample distributed in one or more micro-wells of a measurement device. The sensors utilize narrowband filtering of the measured signal(s), resulting in a large reduction in noise. Synchronous detection is utilized to provide high discrimination of the desired signal from noise or interfering sources. Conductance and by extension impedance is measured by applying a constant alternating current (AC) voltage across the electrodes of each micro-well and measuring the resulting current.

公开(公告)号：US20160103093A1

公开(公告)日：2016-04-14

申请号：US14853026

申请日：2015-09-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew WONG ,  Todd REARICK ,  John DONOHUE

IPC: G01N27/414 ,  G06F19/22 ,  C12Q1/68

CPC classification number: G01N27/4145 ,  G16B30/00

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

Abstract translation: 用于校正核酸并入信号的方法，用于在核酸序列合成中产生的流体潜在效应或紊乱，包括：将多个模板多核苷酸链设置在设置在传感器阵列上的多个限定的空间中，所述模板多核苷酸链具有测序 引物和与之结合的聚合酶; 将模板多核苷酸链暴露于流过流体歧管的一系列核苷酸物质流，该流体歧管包括用于流动核苷酸物质的通道和用于使溶液流动的分支通道，该分支通道包括参考电极和感测电极; 获得对应于所述多个限定空间的多个核苷酸掺入信号，所述核苷酸掺入信号具有与多个核苷酸结合相关的信号强度; 以及校正所述多个核苷酸并入信号中的至少一些用于流体潜在效应或干扰。