公开(公告)号：US20190233887A1

公开(公告)日：2019-08-01

申请号：US16225720

申请日：2018-12-19

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Jon A. HOSHIZAKI ,  Joon Mo YANG ,  Maryam SHARIATI ,  David M. COX ,  Kirk M. HIRANO ,  John BRIDGHAM ,  George Stefan GOLDA ,  Sam Lee WOO

IPC分类号： C12Q1/6869 ,  G01N35/10 ,  B01F13/00 ,  B01F13/02 ,  B01J19/00 ,  C12Q1/6874 ,  B01L3/00 ,  G01N35/00

CPC分类号： C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

摘要： Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 μl). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

公开(公告)号：US20150087530A1

公开(公告)日：2015-03-26

申请号：US14498979

申请日：2014-09-26

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Jon A. HOSHIZAKI ,  Joon Mo YANG ,  Maryam SHARIATI ,  David M. COX ,  Kirk M. HIRANO ,  John BRIDGHAM ,  George Stefan GOLDA ,  Sam Lee WOO

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

摘要： Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 μl). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

摘要翻译： 本文提供了低体积测序系统的各种实施方案。 该系统可以包括具有至少一个具有确定体积（例如小于约100μl）的反应室的低体积流动池。 该系统还可以包括配置成与对应于目标反应室的入口端可逆耦合的自动化试剂输送机构，从而使试剂能够以最小的试剂废物从试剂容器准确地移动到反应室。 流通池可以包括多个反应室（例如6），从而允许多个样品的并行分析。 本文还提供了分析生物分子的各种方法。