公开(公告)号：US20190233887A1

公开(公告)日：2019-08-01

申请号：US16225720

申请日：2018-12-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon A. HOSHIZAKI ,  Joon Mo YANG ,  Maryam SHARIATI ,  David M. COX ,  Kirk M. HIRANO ,  John BRIDGHAM ,  George Stefan GOLDA ,  Sam Lee WOO

IPC: C12Q1/6869 ,  G01N35/10 ,  B01F13/00 ,  B01F13/02 ,  B01J19/00 ,  C12Q1/6874 ,  B01L3/00 ,  G01N35/00

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 μl). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

公开(公告)号：US20150087530A1

公开(公告)日：2015-03-26

申请号：US14498979

申请日：2014-09-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon A. HOSHIZAKI ,  Joon Mo YANG ,  Maryam SHARIATI ,  David M. COX ,  Kirk M. HIRANO ,  John BRIDGHAM ,  George Stefan GOLDA ,  Sam Lee WOO

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 μl). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.

Abstract translation: 本文提供了低体积测序系统的各种实施方案。 该系统可以包括具有至少一个具有确定体积（例如小于约100μl）的反应室的低体积流动池。 该系统还可以包括配置成与对应于目标反应室的入口端可逆耦合的自动化试剂输送机构，从而使试剂能够以最小的试剂废物从试剂容器准确地移动到反应室。 流通池可以包括多个反应室（例如6），从而允许多个样品的并行分析。 本文还提供了分析生物分子的各种方法。

公开(公告)号：US20150191784A1

公开(公告)日：2015-07-09

申请号：US14593749

申请日：2015-01-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David COX ,  Marc HABERSTROH ,  Patrick KINNEY ,  Albert CARRILLO ,  Jon HOSHIZAKI ,  Maryam SHARIATI ,  Howard KING ,  Joe Y. LEE ,  Matthew CHAN

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast-indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser. For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast-indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelengths are also provided herein.

Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括具有多个激发和发射滤波器的快速分度滤光轮，该激发和发射滤光片能够快速旋转进入激发源（例如，弧光灯，激光器） 轮可以被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。该系统还可以被配置为考虑快速启动和停止快速索引所产生的振动 滤光轮以及由其他光源产生的振动。本文还提供了在多个波长下快速成像样品的各种方法。