公开(公告)号：US20150080247A1

公开(公告)日：2015-03-19

申请号：US14385730

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Eliodor Ghenciu ,  Evan W. Foster ,  Jorge Fonseca ,  Kevin Maher

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/50857 ,  B01L3/50 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  C12Q1/6806 ,  C12Q1/686 ,  G03F7/2002

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

Abstract translation: 提供了用于进行生物反应的系统。 该系统包括包括基底和多个反应位点的芯片。 多个反应位点各自被配置为包括至多一个纳升级的液体样品。 此外，该系统包括被配置为启动液体样品内的生物反应的控制系统。 该系统还包括被配置为检测芯片上的生物反应的检测系统。 根据各种实施方案，芯片包括至少20000个反应位点。 在其它实施方案中，芯片包括至少30000个反应位点。

公开(公告)号：US20150072897A1

公开(公告)日：2015-03-12

申请号：US14385735

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Evan W. Foster

IPC: G01N35/10 ,  B01J19/00

CPC classification number: G01N35/1002 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/0038 ,  B01J2219/00599 ,  B01J2219/00619 ,  B01J2219/00637 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2200/12 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0406 ,  C40B60/14

Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/−15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.

Abstract translation: 提供了一种用于将液体样品加载到基底内的多个反应位点的样品装载器。 样品装载器包括第一叶片和联接到第一叶片的第二叶片。 样品装载器还包括在第一叶片和第二叶片之间的流动路径，其构造成将液体样品分配到包括多个反应位点的基底。 此外，在各种实施例中，液体样品与第一和第二叶片具有85 +/- 15度的前进接触角。 此外，从流动路径分配到多个反应位点的液体样品的装载可以基于毛细作用。

公开(公告)号：US20190381502A1

公开(公告)日：2019-12-19

申请号：US16426631

申请日：2019-05-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Eliodor Ghenciu ,  Evan Foster ,  Jorge Fonseca ,  Kevin Maher

IPC: B01L3/00 ,  C12Q1/6806 ,  G03F7/20 ,  C12Q1/686

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

公开(公告)号：US20230201839A1

公开(公告)日：2023-06-29

申请号：US18086416

申请日：2022-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Eliodor Ghenciu ,  Evan Foster ,  Jorge Fonseca ,  Kevin Maher

IPC: B01L3/00 ,  C12Q1/6806 ,  G03F7/20 ,  C12Q1/686

CPC classification number: B01L3/50857 ,  B01L3/50 ,  C12Q1/6806 ,  G03F7/2002 ,  B01L3/50851 ,  C12Q1/686 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2200/142 ,  B01L2300/046 ,  B01L2200/0642 ,  B01L7/52

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

公开(公告)号：US11590506B2

公开(公告)日：2023-02-28

申请号：US16426631

申请日：2019-05-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Eliodor Ghenciu ,  Evan Foster ,  Jorge Fonseca ,  Kevin Maher

IPC: B01L3/00 ,  C12Q1/6806 ,  G03F7/20 ,  C12Q1/686 ,  B01L7/00

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

公开(公告)号：US20150044686A1

公开(公告)日：2015-02-12

申请号：US14385765

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael Pallas ,  James C. Nurse ,  Kevin Maher ,  Jorge Fonseca ,  Eliodor Ghenciu ,  Evan Foster

IPC: C12Q1/68 ,  B01L3/00 ,  G03F7/20

CPC classification number: B01L3/50857 ,  B01L3/50 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  C12Q1/6806 ,  C12Q1/686 ,  G03F7/2002

Abstract: An article for holding a plurality of biological samples includes a substrate a substrate comprising a first surface and an opposing second surface and a plurality of reaction sites in the substrate. Each of the reaction sites extends from an opening in the first surface to an opening in the second surface. The reaction sites comprise a hexagonal shape and are configured to provide sufficient surface tension by capillary action to hold respective biological samples. The reaction sites have a density over at least a portion of the surfaces that is at least 170 holes per square millimeter. At least one of the surfaces may have a surface roughness characterized by an arithmetic average roughness (Ra) that is less than or equal to 5 nanometers.

Abstract translation: 用于保持多个生物样品的制品包括基材，其包含基材中的第一表面和相对的第二表面和多个反应位点。 每个反应位点从第一表面中的开口延伸到第二表面中的开口。 反应位点包括六边形形状并且被配置为通过毛细作用提供足够的表面张力以保持各自的生物样品。 反应部位的表面密度至少在每平方毫米170个孔以上。 至少一个表面可以具有由小于或等于5纳米的算术平均粗糙度（Ra）表征的表面粗糙度。