公开(公告)号：US20210080394A1

公开(公告)日：2021-03-18

申请号：US16967111

申请日：2019-02-05

Applicant: LUMICKS TECHNOLOGIES B.V. ,  STICHTING VU

Inventor： Iddo Heller ,  Anna Elisabeth Christina MEIJERING ,  Gijs Jan Lodewijk WUITE ,  Erwin Johannes Gerard PETERMAN ,  Andreas Sebastian BIEBRICHER

IPC: G01N21/64 ,  G16B15/00 ,  G06K9/32 ,  G06K9/00

Abstract: One aspect of this disclosure relates to a method for determining a presence of at least one guest structure at a host structure. The method comprises a light-sensitive system receiving light from the host structure. The host structure hosts one or more optically active entities at at least one part of the host structure. Herein, the at least one part does not host the at least one guest structure. Furthermore, the optically active entities cause light emission from said at least one part. The method also comprises the light-sensitive system outputting a signal based on the received light. The method further comprises determining a light value based on the output signal. The light value indicates an amount of light from the host structure being incident on the light-sensitive system. The method also comprises determining on the basis of the light value at least one of a quantity and a position of the at least one guest structure at the host structure.

公开(公告)号：US09766180B2

公开(公告)日：2017-09-19

申请号：US14895477

申请日：2014-06-02

Applicant: Stichting VU-VUmc

Inventor： Gijs Jan Lodewijk Wuite ,  Erwin Johannes Gerard Peterman ,  Iddo Heller ,  Gerrit Sitters ,  Andrea Candelli ,  Stefan Walter Hell

IPC: G01N33/00 ,  G01N35/00 ,  G01N21/00 ,  C12Q1/68 ,  G01N21/64 ,  B01L3/00 ,  G02B21/00 ,  G02B21/16 ,  G02B21/32

CPC classification number: G01N21/6428 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0454 ,  C12Q1/6816 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/32 ,  C12Q2565/629

Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

公开(公告)号：US20230366801A1

公开(公告)日：2023-11-16

申请号：US18030034

申请日：2021-10-05

Applicant: Stichting VU ,  LUMICKS CA HOLDING B.V.

Inventor： Andreas Sebastian Biebricher ,  Vadim Bogatyr ,  Gijs Jan Lodewijk Wuite ,  Erwin Johannes Gerard Peterman ,  Iddo Heller ,  Rogier Martijn Reijmers

IPC: G01N15/14 ,  G06T7/60 ,  G06T7/20 ,  G06T7/70 ,  G06V20/69

CPC classification number: G01N15/1404 ,  G01N15/1434 ,  G06T7/60 ,  G06T7/20 ,  G06T7/70 ,  G06V20/698 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1075

Abstract: A method comprises receiving images representing manipulating cellular bodies that includes exerting force pulses to the bodies on a wall surface; analyzing the images to determine the size of the bodies and tracking locations during and after each of force pulses, the tracking locations defining first trajectories of the bodies moving away from the wall surface and trajectories of the bodies moving towards the wall surface; determining densities of the bodies using the second trajectories and a sedimentation model of the bodies moving towards the wall surface and determining body velocities based on the first trajectories and a velocity model of the bodies moving away from the wall surface; and, determining a contrast factor for each body based on the sizes and the densities, the force applied to the bodies and the body velocities and determining a compressibility for each of the bodies based on the determined contrast factors.

公开(公告)号：US09952421B2

公开(公告)日：2018-04-24

申请号：US15549832

申请日：2016-02-04

Applicant: Stichting Vu

Inventor： Andreas Sebastian Biebricher ,  Andrea Candelli ,  Iddo Heller ,  Niels Laurens ,  Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite

IPC: G02B21/32 ,  G02B27/14 ,  G02B26/08 ,  G02B27/28 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G01N21/64 ,  B01L3/00 ,  G02B27/10

CPC classification number: G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1,OT2,OT3). In one method, trapping beams (TB1,TB2,TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1,OT2,OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.

公开(公告)号：US20180024342A1

公开(公告)日：2018-01-25

申请号：US15549832

申请日：2016-02-04

Applicant: Stichting Vu

Inventor： Andreas Sebastian Biebricher ,  Andrea Candelli ,  Iddo Heller ,  Niels Laurens ,  Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite

IPC: G02B21/32 ,  G02B27/14 ,  G02B26/08 ,  B01L3/00 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G01N21/64 ,  G02B27/10 ,  G02B27/28

CPC classification number: G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1, OT2, OT3). In one method, trapping beams (TB1, TB2, TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1, OT2, OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.