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    A NOVEL VECTOR AND EXPRESSION CELL LINE FOR MASS PRODUCTION OF RECOMBINANT PROTEIN AND A PROCESS OF PRODUCING RECOMBINANT PROTEIN USING SAME
    4.
    发明申请
    A NOVEL VECTOR AND EXPRESSION CELL LINE FOR MASS PRODUCTION OF RECOMBINANT PROTEIN AND A PROCESS OF PRODUCING RECOMBINANT PROTEIN USING SAME 有权
    用于生产重组蛋白质的新型载体和表达细胞系以及使用其生产重组蛋白的方法

    公开(公告)号：US20100120089A1

    公开(公告)日：2010-05-13

    申请号：US12444567

    申请日：2007-10-16

    申请人： In Young Choi ,  Chang Hwan Kim ,  Hyun Ji Lee ,  Seong Hee Park ,  Se Chang Kwon ,  Gwan Sun Lee

    发明人： In Young Choi ,  Chang Hwan Kim ,  Hyun Ji Lee ,  Seong Hee Park ,  Se Chang Kwon ,  Gwan Sun Lee

    IPC分类号： C12P21/00 ,  C12N15/74 ,  C12N5/10

    CPC分类号： C12N9/003 ,  C12N15/85 ,  C12N2510/00

    摘要： Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein.

    摘要翻译： 本文公开了包含二氢叶酸还原酶（DHFR）启动子的诱导型高表达盒，从其中部分或全部除去富含GC的重复序列，所述盒能够更有效地改善基因扩增系统。 还公开了包含可诱导表达盒和任选的编码目标重组蛋白的基因，用表达载体转化的动物细胞系的表达载体，以及通过培养转化体大量生产和纯化重组蛋白的方法。 本发明能够缩短使用低浓度的DHFR抑制剂以高水平建立产生重组蛋白质的细胞系所需的时间，从而允许更有效地产生重组蛋白质。

    NERVE REGENERATION
    5.
    发明申请
    NERVE REGENERATION 审中-公开
    神经再生

    公开(公告)号：US20060014288A1

    公开(公告)日：2006-01-19

    申请号：US11160446

    申请日：2005-06-23

    申请人： Chang Hwan Kim ,  Kwan Hee Lee

    发明人： Chang Hwan Kim ,  Kwan Hee Lee

    IPC分类号： A61K48/00 ,  C12N5/08 ,  C12N15/86

    CPC分类号： A61K48/0058 ,  A61K35/30 ,  A61K38/1875 ,  A61K38/1883 ,  A61K48/00 ,  C12N2799/021 ,  C12N2799/022 ,  C12N2799/025 ,  C12N2799/027 ,  C12N2799/028 ,  A61K2300/00

    摘要： The present application describes a method of regenerating nerve, which steps include generating a recombinant viral or plasmid vector comprising a DNA sequence encoding a member of a transforming growth factor superfamily of proteins operatively linked to a promoter; transfecting in vitro a population of cultured cells with the recombinant vector, resulting in a population of the cultured cells; and transplanting the transfected cells to an area near an injured nerve, such that expression of the DNA sequence within the area near the injured nerve causes regeneration of the nerve.

    摘要翻译： 本申请描述了再生神经的方法，该步骤包括产生重组病毒或质粒载体，其包含编码与启动子有效连接的蛋白质的转化生长因子超家族成员的DNA序列; 用重组载体体外转染培养的细胞群，导致培养细胞群体; 并将转染的细胞移植到受伤神经附近的区域，使得DNA序列在受伤神经附近的区域中的表达导致神经再生。

    Method for mass production of factor VII/VIIA
    7.
    发明授权
    Method for mass production of factor VII/VIIA 有权
    大量生产因子VII / VIIA的方法

    公开(公告)号：US09243237B2

    公开(公告)日：2016-01-26

    申请号：US13881974

    申请日：2011-10-26

    申请人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

    发明人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

    IPC分类号： A61K38/00 ,  C12N9/64 ,  C07K14/745

    CPC分类号： C12N9/6437 ,  C07K14/745 ,  C12N15/85 ,  C12N2830/85

    摘要： A method for the mass production of human coagulation Factor VII. The method includes a) providing an expression vector carrying i) a dihydrofolate reductase promoter devoid of one or more CCGCC repeat sequences from the GC-rich region thereof and a dihydrofolate reductase (DHFR) gene operably linked thereto and ii) a cytomegalovirus (CMV) promoter and a human coagulation Factor VII gene operably linked thereto; b) obtaining a transformed host cell line containing the expression vector; and c) culturing the transfected host cell in the presence of a dihydrofolate reductase inhibitor to select cells which express human coagulation Factor VII with high efficiency; and d) adding sodium butyrate to the selected host cells.

    摘要翻译： 大量生产人凝血因子VII的方法。 所述方法包括：a）提供表达载体，其携带i）没有来自其富含GC的区域的一个或多个CCGCC重复序列的二氢叶酸还原酶启动子和与其可操作地连接的二氢叶酸还原酶（DHFR）基因，和ii）巨细胞病毒（CMV） 启动子和与之有效连接的人凝血因子VII基因; b）获得含有表达载体的转化的宿主细胞系; 和c）在二氢叶酸还原酶抑制剂存在下培养转染的宿主细胞以选择以高效率表达人凝血因子VII的细胞; 和d）向所选择的宿主细胞中加入丁酸钠。

    Method for controlling the walk of humanoid robot
    8.
    发明授权
    Method for controlling the walk of humanoid robot 有权
    控制人形机器人步行的方法

    公开(公告)号：US08738178B2

    公开(公告)日：2014-05-27

    申请号：US12090350

    申请日：2005-10-24

    申请人： Young Jin Choi ,  Do Ik Kim ,  Yong Hwan Oh ,  Chang Hwan Kim ,  Bum Jae You ,  Jung San Cho

    发明人： Young Jin Choi ,  Do Ik Kim ,  Yong Hwan Oh ,  Chang Hwan Kim ,  Bum Jae You ,  Jung San Cho

    IPC分类号： G05B19/18

    CPC分类号： B62D57/032

    摘要： The invention relates to a method for controlling walking of humanoid bipedal walking robot. More specifically, the invention comprises steps of designing a zero momentum position (ZMP) of a robot for the ground surface (a); calculating trajectories of a center of gravity (COG) of the robot along with the trajectory of the ZMP (b); calculating an angular velocity of driving motors of two feet, which has the robot walk according to the trajectory of the ZMP (c); and controlling walking of the robot by driving the driving motors according to the angular velocity of the driving motors calculated above. The robot walking control method according to the invention has stability against disturbances.

    摘要翻译： 本发明涉及一种用于控制人形二足步行机器人步行的方法。 更具体地，本发明包括设计用于地面（a）的机器人的零动量位置（ZMP）的步骤。 计算机器人的重心（COG）轨迹与ZMP（b）的轨迹的轨迹; 计算具有根据ZMP（c）的轨迹的机器人行走的两英尺的驱动电动机的角速度; 以及通过根据上述计算的驱动马达的角速度驱动驱动马达来控制机器人的行走。 根据本发明的机器人步行控制方法具有抗干扰的稳定性。