公开(公告)号：US4808704A

公开(公告)日：1989-02-28

申请号：US868872

申请日：1986-05-22

申请人： Lloyd J. Old ,  Herbert F. Oettgen ,  Hisami Ikeda ,  Lucy T. C. Li ,  Kenneth O. Lloyd ,  Wolfgang G. Dippold

发明人： Lloyd J. Old ,  Herbert F. Oettgen ,  Hisami Ikeda ,  Lucy T. C. Li ,  Kenneth O. Lloyd ,  Wolfgang G. Dippold

IPC分类号： C07K16/30 ,  C12N5/02 ,  A61K39/395 ,  C12N5/00 ,  C12N15/00 ,  C12P21/00

CPC分类号： C07K16/3053

摘要： Monoclonal antibody recognizing human melanoma cells, method of production and use.

摘要翻译： 识别人黑素瘤细胞的单克隆抗体，生产和使用方法。

公开(公告)号：US4507391A

公开(公告)日：1985-03-26

申请号：US365065

申请日：1982-04-02

申请人： Clifford S. Pukel ,  Kenneth O. Lloyd ,  Luiz R. Travassos ,  Wolfgang G. Dippold ,  Herbert F. Oettgen ,  Lloyd J. Old

发明人： Clifford S. Pukel ,  Kenneth O. Lloyd ,  Luiz R. Travassos ,  Wolfgang G. Dippold ,  Herbert F. Oettgen ,  Lloyd J. Old

IPC分类号： C12Q1/04 ,  G01N33/53 ,  G01N33/574 ,  G01N33/54 ,  C12N15/00

CPC分类号： G01N33/5743 ,  G01N33/5308 ,  Y10S436/804 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/815 ,  Y10S436/828

摘要： Mouse monoclonal antibody AbR.sub.24 (Dippold et al., Proc. Natl. Acad. Sci. 77:6114-6118, 1980) has a high degree of specificity for human melanoma cells when tested on viable cultured cells using the PA-MHA serological assay. The antigen detected by this antibody has been isolated from melanoma cells and shown to be G.sub.D3 ganglioside by compositional and partial structural analysis and by comparison with authentic G.sub.D3 by thin layer chromatography (TLC). AbR.sub.24 reacts with authentic G.sub.D3, but not with any other ganglioside tested. Using TLC and reactivity with AbR.sub.24, a wide range of cells and tissues was examined for the presence of G.sub.D3. A new serological assay, termed glycolipid-mediated immune adherence (GMIA), was devised for assaying the reactivity of AbR.sub.24 with gangliosides. Melanomas (cultured cells or tumor tissue) were shown to have T.sub.D3 and G.sub.M3 as major gangliosides. Other cells and tissues examined also contained G.sub.D3, but usually only in low amounts. Melanomas (and MOLT-4, a T-cell line) were characterized by a simplified ganglioside profile with G.sub.D3 and G.sub.M3 as major components. The apparent discrepancy between the ubiquitous presence of G.sub.D3 and the serological specificity of AbR.sub.24 for melanoma cells can be explained in terms of localization and concentration of G.sub.D3 in different cells.

摘要翻译： 当使用PA-MHA血清学测定法在活细胞培养细胞上测试时，小鼠单克隆抗体AbR24（Dippold等人，Proc.Natl.Acad.Sci.77：6114-6118,1980）对人黑素瘤细胞具有高度的特异性。 由该抗体检测到的抗原已从黑素瘤细胞中分离出来，通过组成和部分结构分析显示为GD3神经节苷脂，并通过薄层色谱（TLC）与真实的GD3进行比较。 AbR24与正常的GD3反应，但不与任何其他神经节苷脂检测。 使用TLC和与AbR24的反应性，检查广泛的细胞和组织的GD3的存在。 设计了一种称为糖脂介导的免疫粘附（GMIA）的血清学测定法，用于测定AbR24与神经节苷脂的反应性。 黑色素瘤（培养细胞或肿瘤组织）显示具有TD3和GM3作为主要神经节苷脂。 检查的其他细胞和组织也含有GD3，但通常只有少量。 黑色素瘤（和MOLT-4，T细胞系）以GD3和GM3为主要成分的简化神经节苷脂特征表征。 GD3的普遍存在与黑素瘤细胞的AbR24的血清学特异性之间的明显差异可以根据不同细胞中GD3的定位和浓度来解释。

公开(公告)号：US4562160A

公开(公告)日：1985-12-31

申请号：US482153

申请日：1983-04-01

申请人： Francisco X. Real ,  M. Jules Mattes ,  Alan N. Houghton ,  Philip O. Livingston ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old

发明人： Francisco X. Real ,  M. Jules Mattes ,  Alan N. Houghton ,  Philip O. Livingston ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old

IPC分类号： C07K16/30 ,  A61K39/395 ,  A61K39/44 ,  A61K43/00

CPC分类号： C07K16/3053 ,  Y10S435/948 ,  Y10S435/961 ,  Y10S436/804 ,  Y10S436/813 ,  Y10S436/815 ,  Y10S436/823 ,  Y10S530/806 ,  Y10S530/807 ,  Y10S530/828

摘要： The present invention concerns novel immunoprecipitating autologous antibodies which recognize the Class 1 gp90 antigen on melanoma cells. These antibodies, optionally tagged with a chromophoric or radioactive label and immobilized on an inert support, may be used to recognize and isolate the gp90 antigen from melanoma cell extracts. Monoclonal antibodies to melanoma may be screened with the gp90 antigen for those which recognize epitopes other than the FD antigenic system.The cell line containing the gp90 antigen which has been cultured in vitro is a source of gp90 antigen for generation of monoclonal antibodies which will be useful in analyzing the gp90 antigen for those epitopes which may be of diagnostic value in immunoassay of melanoma.

摘要翻译： 本发明涉及识别黑素瘤细胞上的1类gp90抗原的新型免疫沉淀自体抗体。 可以使用任选地标记有发色或放射性标记并固定在惰性载体上的这些抗体来识别和分离来自黑素瘤细胞提取物的gp90抗原。 可以用gp90抗原筛选黑素瘤单克隆抗体，以识别除FD抗原系统以外的表位。 含有体外培养的gp90抗原的细胞系是用于产生单克隆抗体的gp90抗原的来源，其可用于分析可能在黑素瘤免疫测定中具有诊断价值的那些表位的gp90抗原。

公开(公告)号：US4678747A

公开(公告)日：1987-07-07

申请号：US470815

申请日：1983-02-28

申请人： Kenneth O. Lloyd ,  Lloyd J. Old ,  Karl-Anders Karlsson ,  Goran Larson ,  Nicklas Stromberg ,  Jan Thurin ,  Bernd R. Anger ,  Herbert F. Oettgen

发明人： Kenneth O. Lloyd ,  Lloyd J. Old ,  Karl-Anders Karlsson ,  Goran Larson ,  Nicklas Stromberg ,  Jan Thurin ,  Bernd R. Anger ,  Herbert F. Oettgen

IPC分类号： C07K16/30 ,  C07K16/34 ,  C12N15/00

CPC分类号： C07K16/34 ,  C07K16/3023 ,  Y10S435/94 ,  Y10S435/96

摘要： Monoclonal antibodies recognizing the difucosyl-type-2-H antigen on human cells and a method of producing said antibodies are disclosed. The monoclonal antibodies are useful in blood typing and in diagnosis of blood disorders and malignancies involving loss or gain of this H antigen.

摘要翻译： 公开了识别人细胞上二硫代基-2-H抗原的单克隆抗体及其制备方法。 单克隆抗体可用于血液分型和诊断涉及该H抗原损失或增加的血液病症和恶性肿瘤。

公开(公告)号：US4865998A

公开(公告)日：1989-09-12

申请号：US83723

申请日：1987-08-10

申请人： Hans-Joachim Feickert ,  Wolfgang Rettig ,  Karen Chorney ,  Carlos Cordon-Cardo ,  Myron R. Melamed ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Bernd Anger

发明人： Hans-Joachim Feickert ,  Wolfgang Rettig ,  Karen Chorney ,  Carlos Cordon-Cardo ,  Myron R. Melamed ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Bernd Anger

IPC分类号： C07K16/30 ,  G01N33/574

CPC分类号： C07K16/3023 ,  G01N33/57423 ,  Y10S435/948

摘要： A panel of monoclonal antibodies produced from normal human lung fibroblasts and human lung tumors as immunogen is used to diagnose the presence of lung tumors and differentiate between those which are benign and those which are cancerous.

摘要翻译： 使用由正常人肺成纤维细胞和人肺肿瘤作为免疫原产生的一组单克隆抗体来诊断肺肿瘤的存在并区分良性和癌性的那些。

公开(公告)号：US4650756A

公开(公告)日：1987-03-17

申请号：US297814

申请日：1981-08-31

申请人： Lloyd J. Old ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Willet F. Whitmore ,  Jerzy Szkudlarek ,  Connie L. Finstad ,  Donna Morrissey ,  Shun-ichiro Ogata ,  Ryuzo Ueda

发明人： Lloyd J. Old ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Willet F. Whitmore ,  Jerzy Szkudlarek ,  Connie L. Finstad ,  Donna Morrissey ,  Shun-ichiro Ogata ,  Ryuzo Ueda

IPC分类号： C12N15/02 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K14/705 ,  C07K16/00 ,  C07K16/30 ,  C07K19/00 ,  C12N5/10 ,  C12P21/08 ,  C12P21/00 ,  C12N5/00 ,  C12N15/00 ,  C12R1/91

CPC分类号： C07K16/3038 ,  Y10S435/948 ,  Y10S530/808 ,  Y10S530/809 ,  Y10S530/864

摘要： The preparation and use of monoclonal antibodies to human renal tumor cells is described. The monoclonal antibodies bind to glycoproteins of 160Kd, 120Kd and 115Kd, a glycolipid, a HLA heavy chain, group A blood and group B blood antigens.

摘要翻译： 描述了对人肾肿瘤细胞的单克隆抗体的制备和使用。 单克隆抗体结合160Kd，120Kd和115Kd的糖蛋白，糖脂，HLA重链，A组血和B组血抗原。

公开(公告)号：US4579827A

公开(公告)日：1986-04-01

申请号：US474415

申请日：1983-03-11

申请人： Junichi Sakamoto ,  Carlos Cordon-Cardo ,  Eileen Friedman ,  Connie L. Finstad ,  Warren E. Enker ,  Myron R. Melamed ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old

发明人： Junichi Sakamoto ,  Carlos Cordon-Cardo ,  Eileen Friedman ,  Connie L. Finstad ,  Warren E. Enker ,  Myron R. Melamed ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old

IPC分类号： G01N33/574 ,  A61K39/395 ,  C07K14/00 ,  C07K16/00 ,  C07K16/30 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  G01N33/577 ,  C12N5/00 ,  C12N15/00

CPC分类号： C07K16/3046 ,  Y10S435/948 ,  Y10S435/96 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/813 ,  Y10S530/864

摘要： A panel of monoclonal antibodies produced from human gastrointestinal tumors as immunogen is used to diagnose the presence of colon cancer. The antibody panel subsets the human gastrointestinal tract in its reactivity vis-a-vis esophagus, stomach, small intestine and colon. The panel is useful as a diagnostic probe for cancer.

摘要翻译： 使用由人类胃肠肿瘤作为免疫原产生的一组单克隆抗体来诊断结肠癌的存在。 抗体屏障使人胃肠道对食管，胃，小肠和结肠的反应性相对应。 该小组可用作癌症的诊断探针。

公开(公告)号：US5006470A

公开(公告)日：1991-04-09

申请号：US39479

申请日：1987-04-16

申请人： Hiroshi Yamaguchi ,  Koichi Furukawa ,  Philip O. Livingston ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Sheila R. Fortunato

发明人： Hiroshi Yamaguchi ,  Koichi Furukawa ,  Philip O. Livingston ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Sheila R. Fortunato

IPC分类号： A61K38/00 ,  A61K47/48 ,  C07K16/30

CPC分类号： A61K47/48569 ,  A61K47/48623 ,  C07K16/30 ,  C07K16/3053 ,  A61K38/00 ,  Y10S424/808 ,  Y10S435/948 ,  Y10S530/865

摘要： This invention provides a monoclonal antibody, produced by the hybridoma cell line designated GXM1, which specifically binds to a human class 1tumor antigen. This invention also provides a human monoclonal antibody, produced by a hybridoma cell line designated HJM1, which specifically binds to each of the ganglioside antigens GD2, GD3, GM3 and GD1b. This invention further provides a human monoclonal antibody, produced by a hybridoma cell line designated FCM1, which specifically binds to the ganglioside antigens GM3 and GD1a. Finally, this invention provides a human monoclonal antibody, produced by a hybridoma cell line designated DSM1, which specifically binds to a human class 2 tumor protein antigen.

摘要翻译： 本发明提供了由称为GXM1的杂交瘤细胞系产生的单克隆抗体，其特异性结合人类1型肿瘤抗原。 本发明还提供了通过与每种神经节苷脂抗原GD2，GD3，GM3和GD1b特异性结合的称为HJM1的杂交瘤细胞系产生的人单克隆抗体。 本发明还提供了通过特异性结合神经节苷脂抗原GM3和GD1a的称为FCM1的杂交瘤细胞系产生的人单克隆抗体。 最后，本发明提供了通过特异性结合人2型肿瘤蛋白抗原的称为DSM1的杂交瘤细胞系产生的人单克隆抗体。

公开(公告)号：US4762800A

公开(公告)日：1988-08-09

申请号：US604080

申请日：1984-04-26

申请人： Wolfgang Rettig ,  Carolos Cordon-Cardo ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Kenneth O. Lloyd ,  Jennifer Ng

发明人： Wolfgang Rettig ,  Carolos Cordon-Cardo ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Kenneth O. Lloyd ,  Jennifer Ng

IPC分类号： C07K16/30 ,  C12N5/00 ,  A61K39/395 ,  G01N33/533 ,  G01N33/534

CPC分类号： C07K16/30 ,  Y10S530/864

摘要： Antibody-producing hybridoma cell lines made by fusion of NS/1 cells with spleen cells of mice after immunization with human teratocarcinoma cells are presented. Monoclonal antibodies from these cell lines recognize the K4, K2 and P12 antigenic systems and are thus useful in detecting and differentiating between normal and cancerous cells. These monoclonal antibodies are especially useful in pathologic analysis of human tumors, especially teratocarcinomas.

摘要翻译： 提出了通过用人类畸胎癌细胞免疫后将NS / 1细胞与小鼠的脾细胞融合而制备的产生抗体的杂交瘤细胞系。 来自这些细胞系的单克隆抗体识别K4，K2和P12抗原系统，因此可用于检测和鉴别正常细胞与癌细胞。 这些单克隆抗体特别适用于人类肿瘤，特别是畸胎癌的病理分析。

公开(公告)号：US4666845A

公开(公告)日：1987-05-19

申请号：US562465

申请日：1983-12-16

申请人： M. Jules Mattes ,  John L. Lewis, Jr. ,  Kenneth O. Lloyd ,  Lloyd J. Old ,  Carlos Cordon-Cardo

发明人： M. Jules Mattes ,  John L. Lewis, Jr. ,  Kenneth O. Lloyd ,  Lloyd J. Old ,  Carlos Cordon-Cardo

IPC分类号： G01N33/574 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/30 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577 ,  C12N5/00 ,  C12N15/00 ,  G01N33/53

CPC分类号： C07K16/30 ,  Y10S435/948 ,  Y10S436/808 ,  Y10S436/813 ,  Y10S530/808 ,  Y10S530/864

摘要： Mouse monoclonal antibodies to several cell antigens of human ovarian, cervical and endometrial carcinomas have been produced and characterized. The distribution of the antigens was determined by mixed hemagglutination assays on 153 normal and malignant cell cultures of various types, and by immunoperoxidase staining of frozen sections of 27 normal adult and 24 fetal tissues. five monoclonal antibodies representative of five classes of mAb raised to restricted ovarian, cervical and endometrial cells were tested extensively producing mAb reactive with cancer but not normal cells. One such mAb, MF116 was readily detected in the spent culture medium of metabolically radiolabeled cells. These antibodies, reacting with relatively restricted cell surface antigens, are useful in the analysis of epithelial cell differentiation, in cancer diagnosis and therapy and in tissue typing of normal or abnormal cells.

摘要翻译： 人类卵巢癌，子宫颈癌和子宫内膜癌的几种细胞抗原的小鼠单克隆抗体已被制备和表征。 通过混合血细胞凝集测定法对各种类型的153种正常和恶性细胞培养物进行抗原分布，通过免疫过氧化物酶染色法检测27例正常成人和24例胎儿组织的冷冻切片。 代表五类单克隆抗体的五种单克隆抗体阳性细胞被广泛地测试产生与癌症但不是正常细胞反应的mAb。 在代谢放射性标记的细胞的废培养基中容易检测到一种这样的mAb，MF116。 这些抗体与相对受限的细胞表面抗原反应可用于分析上皮细胞分化，癌症诊断和治疗以及正常或异常细胞的组织分型。