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1.
公开(公告)号:US5728925A
公开(公告)日:1998-03-17
申请号:US430257
申请日:1995-04-28
申请人: Luis Herrera-Estrella , Guido Van Den Broeck , Marc Van Montagu , Peter Schreier , Jeff Schell , Hans J. Bohnert , Anthony R. Cashmore , Michael P. Timko , Albert P. Kausch
发明人: Luis Herrera-Estrella , Guido Van Den Broeck , Marc Van Montagu , Peter Schreier , Jeff Schell , Hans J. Bohnert , Anthony R. Cashmore , Michael P. Timko , Albert P. Kausch
IPC分类号: C12N15/09 , A01H1/00 , A01H5/00 , C07H21/04 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/29 , C12N15/82 , C12P21/00 , C12P21/02 , C12R1/91 , A01H5/10 , C12N15/62
CPC分类号: C12N15/8216 , C12N15/8221
摘要: Chimaeric DNA sequence which encodes: 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or polypeptide of a plant and 2) a protein or polypeptide that is heterologous to the transit peptide. The chimaeric DNA sequence can be used as a vector for transforming a plant cell so that a chimaeric precursor of the heterologous protein or polypeptide is produced in the cytoplasm of the cell and the chimaeric precursor then transports the heterologous protein or polypeptide in vivo into a chloroplast of the cell.
摘要翻译: 嵌合DNA序列,其编码:1)叶绿体蛋白或植物多肽的细胞质前体的转运肽,2)与转运肽异源的蛋白质或多肽。 嵌合DNA序列可以用作转化植物细胞的载体,使得在细胞的细胞质中产生异源蛋白质或多肽的嵌合体前体,然后将体内的异源蛋白质或多肽转运到叶绿体中 的细胞。
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2.
公开(公告)号:US5717084A
公开(公告)日:1998-02-10
申请号:US470719
申请日:1995-06-06
申请人: Luis Herrera-Estrella , Guido Van Den Broeck , Marc Van Montagu , Peter Schreier , Jeff Schell , Hans J. Bohnert , Anthony R. Cash more , Michael P. Timko , Albert P. Kausch
发明人: Luis Herrera-Estrella , Guido Van Den Broeck , Marc Van Montagu , Peter Schreier , Jeff Schell , Hans J. Bohnert , Anthony R. Cash more , Michael P. Timko , Albert P. Kausch
IPC分类号: C12N15/09 , A01H1/00 , A01H5/00 , C07H21/04 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/29 , C12N15/82 , C12P21/00 , C12P21/02 , C12R1/91 , C12N5/04 , C12N15/62
CPC分类号: C12N15/8216 , C12N15/8221
摘要: A chimaeric DNA sequence comprising: a) a nucleic acid sequence coding for a chimaeric protein or polypeptide comprising in sequence: 1) a transit peptide of a cytoplasmatic precursor of a chloroplast protein or polypeptide of a plant species, and, 2) a protein or polypeptide of interest which is different from a mature chloroplast protein or polypeptide contained in a natural cytoplasmatic precursor thereof, and, b) a promoter upstream of the nucleic acid sequence recognized by polymerases endogenous to a plant, whereby the chimaeric DNA sequence can be expressed in cells of the plant under the control of the promoter and the protein or polypeptide of interest can be translocated in the chloroplasts of the cells of the plant.
摘要翻译: 一种嵌合DNA序列,其包含:a)编码嵌合蛋白或多肽的核酸序列,其顺序包含:1)叶绿体蛋白或植物物种多肽的胞质前体的转运肽,和2)蛋白质或 不同于其天然细胞质前体中含有的成熟叶绿体蛋白或多肽的目标多肽,和b)通过植物内源性聚合酶识别的核酸序列上游的启动子,由此嵌合DNA序列可以表达于 在启动子控制下的植物细胞和感兴趣的蛋白质或多肽可以转运到植物细胞的叶绿体中。
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3.
公开(公告)号:US6063601A
公开(公告)日:2000-05-16
申请号:US468317
申请日:1995-06-06
申请人: Luis Herrera-Estrella , Guidi Van Den Broeck , Marc Van Montagu , Peter Schreier , Jeff Schell , Hans J. Bohnert , Anthony R. Cash more , Michael P. Timko , Albert P. Kausch
发明人: Luis Herrera-Estrella , Guidi Van Den Broeck , Marc Van Montagu , Peter Schreier , Jeff Schell , Hans J. Bohnert , Anthony R. Cash more , Michael P. Timko , Albert P. Kausch
IPC分类号: C12N15/09 , A01H1/00 , A01H5/00 , C07H21/04 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/29 , C12N15/82 , C12P21/00 , C12P21/02 , C12R1/91 , C12N15/62
CPC分类号: C12N15/8216 , C12N15/8221
摘要: Chimaeric DNA sequence which encodes: 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or polypeptide of a plant and 2) a protein or polypeptide that is heterologous to the transit peptide. The chimaeric DNA sequence can be used as a vector for transforming a plant cell so that a chimaeric precursor of the heterologous protein or polypeptide is produced in the cytoplasm of the cell and the chimaeric precursor then transports the heterologous protein or polypeptide in vivo into a chloroplast of the cell.
摘要翻译: 嵌合DNA序列,其编码:1)叶绿体蛋白或植物多肽的细胞质前体的转运肽,2)与转运肽异源的蛋白质或多肽。 嵌合DNA序列可以用作转化植物细胞的载体,使得在细胞的细胞质中产生异源蛋白质或多肽的嵌合体前体,然后将体内的异源蛋白质或多肽转运到叶绿体中 的细胞。
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公开(公告)号:US06262338B1
公开(公告)日:2001-07-17
申请号:US08903325
申请日:1997-07-17
申请人: Peter Schreier , Thomas Herget , Jeff Schell
发明人: Peter Schreier , Thomas Herget , Jeff Schell
IPC分类号: C12N1529
CPC分类号: C07K14/415 , C12N9/14 , C12N9/2442 , C12N15/8279 , C12Y302/01014
摘要: The present invention relates to DNA isolated from Arachis hypogaea which encodes or hybridizes to DNA which encodes a protein that repels pests. Such DNA is useful in the transformation of vectors, host organisms and plants and for the production of plants which exhibit an increased resistance to pests.
摘要翻译: 本发明涉及从编织消除有害生物的蛋白质的DNA编码或杂交的花生链球菌分离的DNA。 这样的DNA可用于转化载体,宿主生物和植物以及用于产生对害虫具有增加抗性的植物。
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公开(公告)号:US6133506A
公开(公告)日:2000-10-17
申请号:US617860
申请日:1996-05-23
IPC分类号: C07K14/415 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/16 , C12N15/82 , A01H5/00 , C07H21/04
CPC分类号: C12N9/001 , C07K14/415 , C12N15/8222 , C12N9/0006 , C12N9/1029 , C12N9/16 , C12N9/93 , C12Y103/01009 , C12Y103/0101
摘要: Promoters in the 5' non-translated region of genes from Cuphea lanceota that code for .beta.-ketoacyl-(ACP) reductase are disclosed, as well as alleles and derivatives of said promoters. These promoters in the 5' non-translated region may for example be coupled with foreign genes, forming chimeric genes, and be transmitted to plants in appropriate vector systems.
摘要翻译: PCT No.PCT / EP94 / 02950 Sec。 371日期:1996年5月23日 102(e)日期1996年5月23日PCT 1994年9月5日PCT PCT。 公开号WO95 / 07357 日期1995年3月16日公开了编码β-酮酰基 - (ACP)还原酶的来自Cuphea lanceota的5'非翻译区的启动子,以及所述启动子的等位基因和衍生物。 5'非翻译区域中的这些启动子可以例如与外来基因结合,形成嵌合基因,并在适当的载体系统中传递给植物。
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公开(公告)号:US5804184A
公开(公告)日:1998-09-08
申请号:US812025
申请日:1997-03-06
申请人: Jurgen Logemann , Guido Jach , Birgit Gornhardt , John Mundy , Jeff Schell , Peter Eckes
发明人: Jurgen Logemann , Guido Jach , Birgit Gornhardt , John Mundy , Jeff Schell , Peter Eckes
IPC分类号: A01H5/00 , C07K14/37 , C07K14/415 , C12N9/24 , C12N15/82 , A61K38/54 , A61K38/16 , A61K38/47
CPC分类号: C12Y302/01014 , C07K14/37 , C07K14/415 , C12N15/8282 , C12N9/2402 , C12N9/2442 , Y10S47/01
摘要: Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinase (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFP).
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公开(公告)号:US5689045A
公开(公告)日:1997-11-18
申请号:US457797
申请日:1995-06-01
申请人: Jurgen Logemann , Guido Jach , Birgit Gornhardt , John Mundy , Jeff Schell , Peter Eckes , Ilan Chet
发明人: Jurgen Logemann , Guido Jach , Birgit Gornhardt , John Mundy , Jeff Schell , Peter Eckes , Ilan Chet
CPC分类号: C12Y302/01014 , C07K14/37 , C07K14/415 , C12N15/8282 , C12N9/2402 , C12N9/2442 , Y10S47/01
摘要: Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinase (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFP).
摘要翻译: 转基因病原体抗性生物体,其基因组含有至少两种不同基因,在活性启动子控制下具有病原体抑制作用。 该生物体的特征在于协同的病原体抑制作用。 当基因编码基因产物几丁质酶(ChiS,ChiG),葡聚糖酶(GluG),蛋白质合成抑制剂(PSI)和抗真菌蛋白质(AFP))时,这种作用是显而易见的。
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公开(公告)号:US06271438B1
公开(公告)日:2001-08-07
申请号:US09138873
申请日:1998-08-24
申请人: Jürgen Logemann , Guido Jach , Birgit Görnhardt , John Mundy , Jeff Schell , Peter Eckes , Ilan Chet
发明人: Jürgen Logemann , Guido Jach , Birgit Görnhardt , John Mundy , Jeff Schell , Peter Eckes , Ilan Chet
IPC分类号: A01H106
CPC分类号: C12Y302/01014 , C07K14/37 , C07K14/415 , C12N9/2402 , C12N9/2442 , C12N15/8282 , Y10S47/01
摘要: Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinese (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFR).
摘要翻译: 转基因病原体抗性生物体,其基因组含有至少两种不同基因,在活性启动子控制下具有病原体抑制作用。 该生物体的特征在于协同的病原体抑制作用。 当基因编码chitinese(ChiS,ChiG),葡聚糖酶(GluG),蛋白质合成抑制剂(PSI)和抗真菌蛋白质(AFR))的基因时,这一行动是显而易见的。
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公开(公告)号:US5633442A
公开(公告)日:1997-05-27
申请号:US375186
申请日:1995-01-18
申请人: Jeff Schell , Jurgen Logemann , Guido Jach , John Mundy
发明人: Jeff Schell , Jurgen Logemann , Guido Jach , John Mundy
IPC分类号: A01H5/00 , A01N63/00 , A61K38/16 , C07K14/415 , C12N5/10 , C12N9/24 , C12N15/09 , C12N15/29 , C12N15/82 , A01H1/00 , C12P1/04
CPC分类号: C12Y302/01039 , C07K14/415 , C12N15/8282 , C12N9/2402 , C12N9/2442 , C12Y302/01014
摘要: Described are a method of producing pathogen-resistant plants in which a protein-synthesis inhibitor gene or a fusion product of the protein-synthesis inhibitor gene or of the protein-synthesis inhibitor protein with ligands permitting specific attachment to cells is introduced into the genotype of plants under the control of an active promotor, and the use of the protein-synthesis inhibitor protein obtained by introducing the protein-synthesis inhibitor gene into the bacterial overproducers for making pharmaceutical preparations.
摘要翻译: 描述了一种生产病原体抗性植物的方法,其中将蛋白质合成抑制剂基因或蛋白质合成抑制剂基因或蛋白质合成抑制剂蛋白质的融合产物与允许特异性附着于细胞的配体引入基因型 在活性启动子控制下的植物,以及通过将蛋白质合成抑制剂基因导入用于制备药物制剂的细菌过量产生而获得的蛋白质合成抑制剂蛋白质的用途。
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公开(公告)号:USRE39238E1
公开(公告)日:2006-08-15
申请号:US09729141
申请日:2000-12-01
申请人: Jürgen Logemann , Guido Jach , Birgit Görnhardt , John Mundy , Jeff Schell , Peter Eckes , Ilan Chet
发明人: Jürgen Logemann , Guido Jach , Birgit Görnhardt , John Mundy , Jeff Schell , Peter Eckes , Ilan Chet
CPC分类号: C07K14/37 , C07K14/415 , C12N9/24 , C12N15/8282 , C12Y302/01014
摘要: Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinase (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFP).
摘要翻译: 转基因病原体抗性生物体,其基因组含有至少两种不同基因,在活性启动子控制下具有病原体抑制作用。 该生物体的特征在于协同的病原体抑制作用。 当基因编码基因产物几丁质酶(ChiS,ChiG),葡聚糖酶(GluG),蛋白质合成抑制剂(PSI)和抗真菌蛋白质(AFP))时,这种作用是显而易见的。
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