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    Assay for anti transglutaminase antibodies detection useful in celicac disease diagnosis
    1.
    发明授权
    Assay for anti transglutaminase antibodies detection useful in celicac disease diagnosis 失效
    测定抗转谷氨酰胺酶抗体检测可用于celicac疾病诊断

    公开(公告)号：US06905835B2

    公开(公告)日：2005-06-14

    申请号：US09866232

    申请日：2001-05-25

    申请人： Luis Tomás Sorell Gómez ,  Boris Ernesto Acevedo Castro

    发明人： Luis Tomás Sorell Gómez ,  Boris Ernesto Acevedo Castro

    IPC分类号： A61K39/395 ,  G01N33/53 ,  G01N33/564 ,  G01N33/573

    CPC分类号： G01N33/564 ,  G01N33/573 ,  Y10S435/97 ,  Y10S435/971 ,  Y10S436/81

    摘要： A non-instrumental assay for the diagnosis of celiac disease based on the general immunochromatographic assay principles. The assay is rapid and simple and allows the reliable detection of anti transglutaminase antibodies, of both IgA and IgG isotype, in samples of human serum, plasma or blood, using as tracer the antigen transglutaminase conjugated to a colored substance, like colloidal gold or colored latex particles. The conjugated antigen is deposited onto an inert fibrous support, from where it can be released by a liquid sample. The antibodies in the sample react with the conjugated antigen developing an immunocomplex that migrates through a carrier membrane, like nitrocellulose or nylon with a pore size that allows a laminar flow of the reagents, until it reacts with the same antigen transglutaminase immobilized onto a reactive zone of the membrane. As a consequence of this reaction the immunocomplex will be trapped in the reaction site and a colored signal will be seen. Therefore a visually detectable signal in the reactive zone of the membrane indicates a positive result for the detection of anti transglutaminase antibodies in the sample.

    摘要翻译： 基于一般免疫色谱测定原理的用于诊断乳糜泻的非仪器性测定法。 该测定是快速和简单的，并且允许在人血清，血浆或血液样品中可靠地检测IgA和IgG同种型的抗转谷氨酰胺抗体，使用与着色物质缀合的抗原转谷氨酰胺酶，如胶体金或着色剂 胶乳颗粒。 缀合的抗原沉积在惰性纤维载体上，从其可以被液体样品释放。 样品中的抗体与共轭抗原反应，发生免疫复合物，其通过载体膜迁移，如硝化纤维素或尼龙，其孔径允许试剂层流，直到其与固定在反应区上的相同抗原转谷氨酰胺酶反应 的膜。 作为该反应的结果，免疫复合物将被捕获在反应位点，并且将看到有色信号。 因此，膜的反应性区域中的视觉上可检测的信号指示样品中抗转谷氨酰胺酶抗体检测的阳性结果。