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    Modified .beta..sub.2 -microglobulin
    6.
    发明授权
    Modified .beta..sub.2 -microglobulin 失效
    改良的β2-微球蛋白

    公开(公告)号：US5051371A

    公开(公告)日：1991-09-24

    申请号：US3436

    申请日：1987-01-15

    申请人： Mogens H. Nissen ,  Jesper Zeuthen ,  Flemming S. Larsen ,  Lars Thim ,  Mogens Christensen

    发明人： Mogens H. Nissen ,  Jesper Zeuthen ,  Flemming S. Larsen ,  Lars Thim ,  Mogens Christensen

    IPC分类号： C07K14/005 ,  A61K38/00 ,  A61K38/16 ,  A61K39/00 ,  A61P37/00 ,  C07K14/00 ,  C07K14/195 ,  C07K14/435 ,  C07K14/74 ,  C07K16/00 ,  C07K16/28 ,  C07K19/00 ,  C12P21/08 ,  G01N33/534 ,  G01N33/569 ,  G01N33/577 ,  G01N33/68

    CPC分类号： G01N33/6893 ,  C07K14/70539 ,  C07K16/2833 ,  A61K38/00 ,  C07K2317/34 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/864

    摘要： Substantially pure modified .beta..sub.2 -microglobulin (m.beta..sub.2 m) of the formula I ##STR1## wherein R.sub.1 is 24-amino acid residue, with the sequence Ile-Gln-Arg-Thr-Pro-Lys-Ile-Gln-Val-Tyr-Ser-Arg-His-Pro-Ala-Glu-Asn-Gly-Lys-Ser-Asn-Phe-Leu-Asn, R.sub.2 is a 30-amino acid residue with the sequence Tyr-Val-Ser-Gly-Phe-His-Pro-Ser-Asp-Ile-Glu-Val-Asp-Leu-Leu-Lys-Asn-Gly-Glu-Arg-Ile-Gly-Lys-Val-Glu-His-Ser-Asp-Leu-Ser, R.sub.3 is a 20-amino acid residue with the sequence Trp-Ser-Phe-Tyr-Leu-Leu-Tyr-Tyr-Glu-Phe-Thr-Pro-Thr-Glu-Lys-Asp-Glu-Tyr-Ala, R.sub.4 is a 19-amino acid residue with the sequence Arg-Val-Asn-His-Val-Thr-Leu-Ser-Gln-Pro-Lys-Ile-Val-Lys-Trp-Asp-Arg-Asp-Met, X is Phe, Phe-Ser, or Phe-Ser-Lys, and Y is Asp, Lys-Asp, or Ser-Lys-Asp is disclosed. The presence of the protein in body fluids is a diagnostic and/or prognostic marker for the development of a variety of disorders such as different types of cancer and diseases involving the immune system. Also disclosed are specific anti-m.beta..sub.2 m antibodies used for the detection and quantification of m.beta..sub.2 m.

    摘要翻译： 具有Ile-Gln-Arg-Thr-Pro-Lys-Ile-Gln-Val-I的序列的基本上纯的经修饰的β2-微球蛋白（mβ2m），其中R1是24-氨基酸残基， Tyr-Ser-Arg-His-Pro-Ala-Glu-Asn-Gl y-Lys-Ser-Asn-Phe-Leu-Asn，R2是具有序列Tyr-Val-Ser-Gly- Phe-His-Pro-Ser-Asp-Ile-Glu-Val-Asp-Leu-Leu-Lys-Asn-Gl y-Glu-Arg-Ile-Gly-Lys-Val-Glu-His-Ser-Asp-Leu -Ser，R3是具有序列Trp-Ser-Phe-Tyr-Leu-Leu-Tyr-Tyr-Glu-Phe-Thr-Pro-Thr-Glu-Lys-Asp-Glu-Tyr的20个氨基酸残基 -Ala，R4是具有序列Arg-Val-Asn-His-Val-Thr-Leu-Ser-Gln-Pro-Lys-Ile-Val-Lys-Trp-Asp-Arg-Asp的19个氨基酸残基 -Met，X是Phe，Phe-Ser或Phe-Ser-Lys，Y是Asp，Lys-Asp或Ser-Lys-Asp。 体液中蛋白质的存在是用于发展各种疾病如诊断和/或预后的标志物，例如不同类型的癌症和涉及免疫系统的疾病。 还公开了用于检测和定量m 2 m的特异性抗-mβ2m抗体。

    Purification of Factor VIII Using a Mixed-Mode or Multimodal Resin
    9.
    发明申请
    Purification of Factor VIII Using a Mixed-Mode or Multimodal Resin 有权
    使用混合模式或多模态树脂纯化因子VIII

    公开(公告)号：US20100204452A1

    公开(公告)日：2010-08-12

    申请号：US12668530

    申请日：2008-07-11

    申请人： Susanne Bang ,  Lars Thim ,  Johan Karlsson

    发明人： Susanne Bang ,  Lars Thim ,  Johan Karlsson

    IPC分类号： C07K1/14

    CPC分类号： C07K14/755

    摘要： A method for purifying a recombinant protein using a multimodal or mixed mode resin containing ligands which comprise a hydrophobic part and a negatively charged part is described. The invention is advantageous in that it is a single step chromatographic process which does not require adjustment of pH or conductivity during loading step and results in high yield and potency. The process is used for the purification of recombinant compositions of coagulation factor, particularly recombinant Factor VIII.

    摘要翻译： 描述了使用含有包含疏水部分和带负电荷部分的配体的多峰或混合模式树脂来纯化重组蛋白的方法。 本发明的优点在于它是单步色谱方法，其不需要在加载步骤期间调节pH或电导率，并导致高产率和效力。 该方法用于纯化凝血因子，特别是重组因子VIII的重组组合物。

    Human Spasmolytic Polypeptide in Glycosylated Form
    10.
    发明申请
    Human Spasmolytic Polypeptide in Glycosylated Form 审中-公开
    人解脂多糖在糖基化形式

    公开(公告)号：US20090011461A1

    公开(公告)日：2009-01-08

    申请号：US12040141

    申请日：2008-02-29

    申请人： Lars Thim ,  Kjeld Norris ,  Fanny Norris ,  Soren Erik Bjorn ,  Mogens Christensen ,  Per Franklin Nielsen

    发明人： Lars Thim ,  Kjeld Norris ,  Fanny Norris ,  Soren Erik Bjorn ,  Mogens Christensen ,  Per Franklin Nielsen

    IPC分类号： C12P21/04

    CPC分类号： C07K14/575 ,  A61K38/00

    摘要： Human spasmolytic polypeptide (HSP) which has the amino acid sequence Glu Lys Pro Ser Pro Cys Gln Cys Ser Arg Leu Ser Pro His Asn Arg Thr Asn Cys Gly Phe Pro Gly Ile Thr Ser Asp Gln Cys Phe Asp Asn Gly Cys Cys Phe Asp Ser Ser Val Thr Gly Val Pro Trp Cys Phe His Pro Leu Pro Lys Gln Glu Ser Asp Gln Cys Val Met Glu Val Ser Asp Arg Arg Asn Cys Gly Tyr Pro Gly Ile Ser Pro Glu Glu Cys Ala Ser Arg Lys Cys Cys Phe Ser Asn Phe Ile Phe Glu Val Pro Trp Cys Phe Phe Pro Asn Ser Val Glu Asp Cys His Tyror a functionally equivalent homologue thereof, characterized by being in glycosylated form.

    摘要翻译： 具有氨基酸序列的人解痉多肽（HSP）Glu Lys Pro Ser Pro Cys Gln Cys Ser Arg Leu Ser Pro His Asn Arg Thr Asn Cys Gly Phe Pro Gly Ile Thr Ser Asp Gln Cys Phe Asp Asn Gly Cys Cys Phe Asp Ser Ser Val Thr Gly Val Pro Trp Cys Phe His Pro Leu Pro Lys Gln Glu Ser Asp Gln Cys Val Met Glu Val Ser Asp Arg Arg Asn Cys Gly Tyr Pro Gly Ile Ser Pro Glu Glu Cys Ala Ser Arg Lys Cys Cys Phe Ser Asn Phe Ile Phe Glu Val Pro Trp Cys Phe Phe Pro Asn Ser Val Glu Asp Cys His Tyr或其功能等同同系物，其特征在于呈糖基化形式。