公开(公告)号：US08730459B2

公开(公告)日：2014-05-20

申请号：US13501941

申请日：2010-10-15

申请人： Makoto Nogami ,  Katsuhiro Kanda ,  Shinya Ito ,  Izumi Waki

发明人： Makoto Nogami ,  Katsuhiro Kanda ,  Shinya Ito ,  Izumi Waki

IPC分类号： G01N33/48

CPC分类号： G01N35/025 ,  G01N1/405 ,  G01N1/4077 ,  G01N2035/00485 ,  G01N2035/0427 ,  G01N2035/0441

摘要： Provided herein are an examination device and an examination method that can efficiently perform examination even when both whole blood and serum/blood plasma specimens are to be examined. A solid-phase extraction cartridge or a filter is placed on a continuous track of a cartridge table. A cup table is disposed below the cartridge table. A cup is placed on a continuous track of the cup table to receive a sample purified by the filter. When viewed from above, the continuous track of the cartridge table and the continuous track of the cup table cross each other at a position “m”, and each of the tracks crosses a position at which a sample probe operates. A pretreatment for serum/blood plasma is completed in one rotation of the cartridge table. A pretreatment for whole blood is completed in two rotations of the cartridge table.

摘要翻译： 这里提供即使在检查全血和血清/血浆样本时也能够有效地进行检查的检查装置和检查方法。 固相提取盒或过滤器放置在墨盒台的连续轨道上。 一个杯子桌子放置在墨盒桌子下方。 将杯子放置在杯台的连续轨道上以接收由过滤器净化的样品。 当从上方观察时，墨盒台的连续轨道和杯台的连续轨道在位置“m”处彼此交叉，并且每个轨道与样品探针操作的位置相交。 血清/血浆的预处理在药筒表的一个旋转中完成。 全血的预处理在墨盒桌子的两个旋转中完成。

公开(公告)号：US20130130401A1

公开(公告)日：2013-05-23

申请号：US13704541

申请日：2011-06-10

申请人： Katsuhiro Kanda ,  Makoto Nogami ,  Shinya Ito ,  Izumi Waki

发明人： Katsuhiro Kanda ,  Makoto Nogami ,  Shinya Ito ,  Izumi Waki

IPC分类号： G01N1/34

CPC分类号： G01N1/34 ,  G01N1/405 ,  G01N2035/1053 ,  Y10T436/255

摘要： A sample treatment apparatus is disclosed that allows a filtrate after reaction to be supplied to a solid phase extraction treatment without temporary collection. A necessary amount of a solid phase extracting agent is added to a solid phase extraction device for conditioning. An aqueous solution is added to the extracting agent and supernatants are discarded. A filter device is attached to an opening of the extraction and a sample is added into a filter reservoir. A hemolyzing agent is added to the sample, and they are stirred sufficiently to cause disruption of blood cells. A protein-denaturing solution is added to the mixture, and they stirred sufficiently to denature proteins contained in the sample. They are centrifuged to leave aggregate in the filter device, and the filtrate is supplied to the solid phase extracting agent through a filter unit without collecting the filtrate.

摘要翻译： 公开了一种样品处理装置，其允许将反应后的滤液供应至固相萃取处理而不进行临时收集。 将必要量的固相萃取剂加入到用于调理的固相萃取装置中。 向提取剂中加入水溶液，弃去上清液。 过滤装置连接到提取物的开口，并将样品加入到过滤器容器中。 将溶血剂加入到样品中，并充分搅拌以引起血细胞破裂。 将蛋白质变性溶液加入到混合物中，并充分搅拌以使包含在样品中的蛋白质变性。 将它们离心以将聚集体留在过滤装置中，并将滤液通过过滤装置供应至固相萃取剂，而不收集滤液。

公开(公告)号：US20120206713A1

公开(公告)日：2012-08-16

申请号：US13501941

申请日：2010-10-15

申请人： Makoto Nogami ,  Katsuhiro Kanda ,  Shinya Ito ,  Izumi Waki

发明人： Makoto Nogami ,  Katsuhiro Kanda ,  Shinya Ito ,  Izumi Waki

IPC分类号： G01N33/48

CPC分类号： G01N35/025 ,  G01N1/405 ,  G01N1/4077 ,  G01N2035/00485 ,  G01N2035/0427 ,  G01N2035/0441

摘要： Provided herein are an examination device and an examination method that can efficiently perform examination even when both whole blood and serum/blood plasma specimens are to be examined. A solid-phase extraction cartridge or a filter is placed on a continuous track of a cartridge table. A cup table is disposed below the cartridge table. A cup is placed on a continuous track of the cup table to receive a sample purified by the filter. When viewed from above, the continuous track of the cartridge table and the continuous track of the cup table cross each other at a position “m”, and each of the tracks crosses a position at which a sample probe operates. A pretreatment for serum/blood plasma is completed in one rotation of the cartridge table. A pretreatment for whole blood is completed in two rotations of the cartridge table.

摘要翻译： 这里提供即使在检查全血和血清/血浆样本时也能够有效地进行检查的检查装置和检查方法。 固相提取盒或过滤器放置在墨盒台的连续轨道上。 一个杯子桌子放置在墨盒桌子下方。 将杯子放置在杯台的连续轨道上以接收由过滤器净化的样品。 当从上方观察时，墨盒台的连续轨道和杯台的连续轨道在位置“m”处彼此交叉，并且每个轨道与样品探针操作的位置相交。 血清/血浆的预处理在药筒表的一个旋转中完成。 全血的预处理在墨盒桌子的两个旋转中完成。

公开(公告)号：US20110287446A1

公开(公告)日：2011-11-24

申请号：US13146952

申请日：2010-02-09

申请人： Katsuhiro Kanda ,  Makoto Nogami ,  Izumi Waki

发明人： Katsuhiro Kanda ,  Makoto Nogami ,  Izumi Waki

IPC分类号： G01N33/53 ,  C12M1/34 ,  G01N33/566 ,  G01N30/00

CPC分类号： G01N33/6848

摘要： An immunoanalytical system in which, after a sample such as a patient's serum is subjected to a pretreatment by an immunological pretreatment device, the sample is subjected to light detection by an immunological photometric detection system. Subsequently, the mass spectrometric pretreatment device performs a pretreatment, and the mass spectrometric detection system performs mass spectrometry. The mass spectrometric detection system performs mass spectrometry on components contained in a supernatant. A signal intensity and peak area for each of components are calculated from an obtained chromatograph. A quantitative value measured based on the immunoanalytical method is calculated for each of the components on the basis of the relative ratios of the components.

摘要翻译： 一种免疫分析系统，其中在诸如患者血清的样品经由免疫预处理装置进行预处理之后，通过免疫光度检测系统对样品进行光检测。 随后，质谱预处理装置进行预处理，质谱检测系统进行质谱分析。 质谱检测系统对上清液中所含的组分进行质谱分析。 从获得的色谱图中计算各组分的信号强度和峰面积。 基于组分的相对比例，对每个组分计算基于免疫分析方法测量的定量值。

公开(公告)号：US20120058009A1

公开(公告)日：2012-03-08

申请号：US13201659

申请日：2010-01-18

申请人： Makoto Nogami ,  Yuichiro Hashimoto ,  Izumi Waki ,  Katsuhiro Kanda

发明人： Makoto Nogami ,  Yuichiro Hashimoto ,  Izumi Waki ,  Katsuhiro Kanda

IPC分类号： G01N33/48

CPC分类号： G01N33/49 ,  G01N1/4055 ,  G01N2001/4083 ,  H01J49/0009 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/0436

摘要： An analyzer includes a pretreatment device and a mass spectrometer. The pretreatment device includes a solid phase extraction mechanism. The mass spectrometer performs mass spectrometry on a sample pretreated by the pretreatment device and then subjected to ionization. The analyzer also includes a storage unit that stores data on dependence of signal intensities of the analyte substance to be measured and the internal standard upon the concentration of a substance inhibiting ionization in the sample, and that stores data on a recovery rate. The analyzer also includes a correcting unit that corrects measurement results of the sample and the internal standard on the basis of the data stored in the storage unit.

摘要翻译： 分析仪包括预处理装置和质谱仪。 预处理装置包括固相提取机构。 质谱仪对由预处理装置预处理的样品进行质谱分析，然后进行电离。 分析仪还包括存储单元，其存储关于待测量的待分析物质的信号强度与内标的数据，所述数据在样品中抑制电离物质的浓度，并且存储关于回收率的数据。 分析器还包括校正单元，其基于存储在存储单元中的数据来校正样本的测量结果和内部标准。

公开(公告)号：US09207152B2

公开(公告)日：2015-12-08

申请号：US13377154

申请日：2010-06-24

申请人： Makoto Nogami ,  Katsuhiro Kanda ,  Izumi Waki

发明人： Makoto Nogami ,  Katsuhiro Kanda ,  Izumi Waki

IPC分类号： G01N33/48 ,  G01N1/40 ,  G01N35/02 ,  G01N30/00 ,  H01J49/00

CPC分类号： G01N1/4055 ,  G01N35/025 ,  G01N2001/4061 ,  G01N2030/009 ,  H01J49/00

摘要： A biological sample preprocessing apparatus includes solid-phase extraction cartridges which purify/concentrate specific constituents of a biological sample, cartridge holders which hold one of the solid-phase extraction cartridges, a cartridge transport that carries the cartridge holders along a continuous track surface, a sample probe that discharges the biological sample into the solid-phase extraction cartridge held in the cartridge holders, a water-based solvent probe that discharges a water-based solvent into the solid-phase extraction cartridge held in the cartridge holder, an organic solvent probe that discharges an organic solvent into the solid-phase extraction cartridge held in the cartridge holder, a pressure loader that applies an air pressure load to the solid-phase extraction cartridge held in the cartridge holder, and a control unit that controls operation of the cartridge transport, the sample probe, the solvent probes, and the pressure loader, wherein sample addition and solvent addition alternate with pressure loading.

摘要翻译： 生物样品预处理装置包括净化/浓缩生物样品的特定组分的固相萃取盒，固定一个固相萃取筒的盒保持器，沿连续轨迹表面承载盒保持器的盒输送器 将生物样品排出到保持在盒保持器中的固相提取盒中的样品探针，将水性溶剂排放到保持在盒保持器中的固相萃取盒中的水性溶剂探针，有机溶剂探针 将有机溶剂排放到保持在盒保持器中的固相提取盒中，对保持在盒保持器中的固相提取盒施加空气压力负荷的压力装载器和控制盒的操作的控制单元 运输，样品探针，溶剂探针和压力装载机，其中样品添加 溶剂添加与压力负荷交替。

公开(公告)号：US20120079875A1

公开(公告)日：2012-04-05

申请号：US13377154

申请日：2010-06-24

申请人： Makoto Nogami ,  Katsuhiro Kanda ,  Izumi Waki

发明人： Makoto Nogami ,  Katsuhiro Kanda ,  Izumi Waki

IPC分类号： G01N1/10 ,  G01N1/28

CPC分类号： G01N1/4055 ,  G01N35/025 ,  G01N2001/4061 ,  G01N2030/009 ,  H01J49/00

摘要： A biological sample preprocessing apparatus includes solid-phase extraction cartridges which purify/concentrate specific constituents of a biological sample, cartridge holders which hold one of the solid-phase extraction cartridges, a cartridge transport that carries the cartridge holders along a continuous track surface, a sample probe that discharges the biological sample into the solid-phase extraction cartridge held in the cartridge holders, a water-based solvent probe that discharges a water-based solvent into the solid-phase extraction cartridge held in the cartridge holder, an organic solvent probe that discharges an organic solvent into the solid-phase extraction cartridge held in the cartridge holder, a pressure loader that applies an air pressure load to the solid-phase extraction cartridge held in the cartridge holder, and a control unit that controls operation of the cartridge transport, the sample probe, the solvent probes, and the pressure loader, wherein sample addition and solvent addition alternate with pressure loading.

摘要翻译： 生物样品预处理装置包括净化/浓缩生物样品的特定组分的固相萃取盒，固定一个固相萃取筒的盒保持器，沿连续轨迹表面承载盒保持器的盒输送器 将生物样品排出到保持在盒保持器中的固相提取盒中的样品探针，将水性溶剂排放到保持在盒保持器中的固相萃取盒中的水性溶剂探针，有机溶剂探针 将有机溶剂排放到保持在盒保持器中的固相提取盒中，对保持在盒保持器中的固相提取盒施加空气压力负荷的压力装载器和控制盒的操作的控制单元 运输，样品探针，溶剂探针和压力装载机，其中样品添加 溶剂添加与压力负荷交替。

公开(公告)号：US08926903B2

公开(公告)日：2015-01-06

申请号：US13060164

申请日：2009-07-08

申请人： Makoto Nogami ,  Katsuhiro Kanda ,  Izumi Waki

发明人： Makoto Nogami ,  Katsuhiro Kanda ,  Izumi Waki

IPC分类号： G01N1/28 ,  G01N1/40 ,  G01N30/00

CPC分类号： G01N1/405 ,  G01N2030/009 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/115831 ,  Y10T436/25 ,  Y10T436/255

摘要： To perform accurate measurement in the analysis of chemical components with low concentration, pretreatment such as concentration and purification of samples is essential. For high-throughput of pretreatment of biological samples, various random clinical testing is encountered, where analytes change from sample to sample. The pretreatment apparatus has a separating agent selectively separating a specific component by allowing a sample solution to flow therethrough. A holding section holds a plurality of housing sections, which house the separating agent therein, and has an endless track. A pressurizing section applies pressure to the housing section in a continuous and random-accessible manner; and an extraction solution receiver mechanism selectively receives an extracted solution from the separating agent housed in the housing section. A mass spectrometer is that can be connected to the pretreatment apparatus. Thus, a large number of specimens can be simultaneously processed.

摘要翻译： 为了在低浓度化学成分的分析中进行精确测量，预处理如样品的浓缩和纯化至关重要。 对于生物样品预处理的高通量，遇到各种随机临床试验，其中分析物从样品变为样品。 预处理装置具有通过允许样品溶液流过其而选择性分离特定成分的分离剂。 保持部保持容纳分离剂的多个容纳部，并具有环状轨迹。 加压部以连续且随机可及的方式对壳体部施加压力; 并且提取溶液接收器机构选择性地从容纳在容纳部分中的分离剂接收提取的溶液。 质谱仪可以连接到预处理装置。 因此，可以同时处理大量的样品。

公开(公告)号：US08319179B2

公开(公告)日：2012-11-27

申请号：US13146802

申请日：2010-07-28

申请人： Katsuhiro Kanda ,  Makoto Nogami ,  Izumi Waki

发明人： Katsuhiro Kanda ,  Makoto Nogami ,  Izumi Waki

IPC分类号： G01N1/10 ,  G01N1/28 ,  G01N1/02 ,  G01N33/48 ,  H01J49/26

CPC分类号： G01N1/4077 ,  G01N1/405 ,  G01N30/82 ,  G01N2030/009 ,  H01J49/0431 ,  H01J49/26

摘要： The present invention relates to a pretreatment apparatus that performs concentration and separation of a sample, and in particular, in order to provide a sample pretreatment apparatus using a solid-phase extraction column, and a mass spectrometer using the same, which is particularly suitable for clinical analysis in which qualitative/quantitative analysis of a biological sample such as blood is performed, according to each operational step for a solid-phase extraction treatment, for example, a collection device serving as flow passages or containers for collection of waste liquid or extracted matter is installed on a bottom face of the solid-phase extraction column, and the extracted matter is separately collected without being mixed with waste liquid by switching the positions of the collection device.

摘要翻译： 本发明涉及一种进行样品浓缩分离的预处理装置，特别是为了提供使用固相萃取柱的样品预处理装置和使用其的质谱仪，特别适用于 根据固相萃取处理的每个操作步骤，例如用作流动通道的收集装置或用于收集废液或提取的容器的容器，进行诸如血液的生物样品的定性/定量分析的临床分析 物质被安装在固相萃取塔的底面上，并且通过切换收集装置的位置而分离地收集提取物而不与废液混合。

公开(公告)号：US20120121464A1

公开(公告)日：2012-05-17

申请号：US13146656

申请日：2010-01-13

申请人： Makoto Nogami ,  Katsuhiro Kanda ,  Izumi Waki

发明人： Makoto Nogami ,  Katsuhiro Kanda ,  Izumi Waki

IPC分类号： G01N27/72 ,  B01L3/00

CPC分类号： G01N1/405 ,  G01N35/00663 ,  G01N35/02 ,  G01N35/025 ,  Y10T436/115831

摘要： A clinical laboratory apparatus includes a pretreating apparatus having a high separation capability and providing high data reproducibility and reliability and a mass spectrometer is provided which executes fully automatic processing from pretreatment to detection. The pretreating apparatus that includes a cartridge for holding an extraction agent for solid-phase extraction, a pressure-loading unit for applying a pressure load to any cartridge mounted on the solid-phase extraction cartridge-retaining unit, a receiving tray mechanism for receiving a sample extracted from the cartridge, and a liquid-level sensor for detecting liquid levels in the receiving tray as well as in the cartridge, conducts feedback control for the pressure-loading unit to open its pressure release valve upon the liquid-level sensor detecting that the liquid level in the receiving tray mechanism has reached a preset liquid-level position.

摘要翻译： 临床实验室装置包括具有高分离能力并且提供高数据再现性和可靠性的预处理装置，并且提供执行从预处理到检测的全自动处理的质谱仪。 该预处理装置包括用于保持固相萃取提取剂的盒，用于对安装在固相提取盒保持单元上的任何盒施加压力负载的压力加载单元，用于接收 从墨盒提取的样品和用于检测接收托盘以及墨盒中的液位的液位传感器，对液位传感器检测到压力加载单元进行反馈控制以打开其压力释放阀， 接收托盘机构中的液位达到预设的液位位置。