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    DEVICE FOR SAMPLING CELLS BY CONTACT
    1.
    发明申请
    DEVICE FOR SAMPLING CELLS BY CONTACT 审中-公开
    联系人采集细胞的装置

    公开(公告)号：US20090317835A1

    公开(公告)日：2009-12-24

    申请号：US12307195

    申请日：2007-07-12

    申请人： Marie-Line Cosnier ,  Patrice Caillat ,  Franck Martin ,  François Berger

    发明人： Marie-Line Cosnier ,  Patrice Caillat ,  Franck Martin ,  François Berger

    IPC分类号： G01N33/567 ,  A61B10/02

    CPC分类号： A61B10/02 ,  A61B10/0291 ,  A61B2010/0216 ,  A61B2017/320008

    摘要： A microtechnological device (6) for sampling cells on or in a tissue or other element of the body. It comprises a support (12) having at least one surface of interest (14) on which is present at least one recovery zone (10) comprised of a bottom wall (22) equipped with a plurality of protuberances (24). Preferably, the microtechnological device comprises at least one recovery zone covered with a coating that has the power to attract the cells to be sampled.

    摘要翻译： 一种微技术装置（6），用于在身体的组织或其他元件上或组织中采集细胞。 它包括具有至少一个感兴趣的表面（14）的支撑件（12），在该至少一个表面上存在至少一个由配备有多个突起（24）的底壁（22）组成的回收区（10）。 优选地，微技术装置包括至少一个被涂覆层覆盖的回收区域，该涂层具有吸引待采样单元的功率。

    Device for contact molecular sampling
    2.
    发明授权
    Device for contact molecular sampling 有权
    接触分子取样装置

    公开(公告)号：US08152736B2

    公开(公告)日：2012-04-10

    申请号：US11814730

    申请日：2006-02-02

    申请人： Patrice Caillat ,  Franck Martin ,  Marie-Line Cosnier

    发明人： Patrice Caillat ,  Franck Martin ,  Marie-Line Cosnier

    IPC分类号： A61B10/00

    CPC分类号： A61B10/02 ,  A61B10/0291 ,  A61B10/04 ,  A61B2010/0216

    摘要： A device (6) to sample molecules of biological interest is described. The device (6) includes capture zones (10) whose developed surface area is much greater than the surface so that sampling by contact allows a sufficient quantity of molecules to be obtained to achieve reliable analysis, whilst being sized so that the device (6) is neither invasive nor aggressive.In particular, the device comprises tiered capture zones (10) located on a support (12) fabricated using microtechnology techniques; the capture zones (10) may advantageously be functionalised.

    摘要翻译： 描述了对生物学兴趣的分子进行样品的装置（6）。 装置（6）包括其显影表面积远大于表面的捕获区（10），使得通过接触进行取样允许获得足够数量的分子以实现可靠的分析，同时尺寸使得装置（6） 既不侵略也不侵略。 特别地，该装置包括位于使用微技术技术制造的支撑件（12）上的分层捕获区（10）; 捕获区（10）可以有利地被功能化。

    DEVICE FOR CONTACT MOLECULAR SAMPLING
    3.
    发明申请
    DEVICE FOR CONTACT MOLECULAR SAMPLING 有权
    用于接触分子采样的装置

    公开(公告)号：US20100049083A1

    公开(公告)日：2010-02-25

    申请号：US11814730

    申请日：2006-02-02

    申请人： Patrice Caillat ,  Franck Martin ,  Marie-Line Cosnier

    发明人： Patrice Caillat ,  Franck Martin ,  Marie-Line Cosnier

    IPC分类号： A61B10/04

    CPC分类号： A61B10/02 ,  A61B10/0291 ,  A61B10/04 ,  A61B2010/0216

    摘要： A device (6) to sample molecules of biological interest is described. The device (6) includes capture zones (10) whose developed surface area is much greater than the surface so that sampling by contact allows a sufficient quantity of molecules to be obtained to achieve reliable analysis, whilst being sized so that the device (6) is neither invasive nor aggressive.In particular, the device comprises tiered capture zones (10) located on a support (12) fabricated using microtechnology techniques; the capture zones (10) may advantageously be functionalised.

    摘要翻译： 描述了对生物学兴趣的分子进行样品的装置（6）。 装置（6）包括其显影表面积远大于表面的捕获区（10），使得通过接触进行取样允许获得足够数量的分子以实现可靠的分析，同时尺寸使得装置（6） 既不侵略也不侵略。 特别地，该装置包括位于使用微技术技术制造的支撑件（12）上的分层捕获区（10）; 捕获区（10）可以有利地被功能化。

    pyrC as a selection marker in Escherichia coli
    5.
    发明授权
    pyrC as a selection marker in Escherichia coli 有权
    pyrC作为大肠杆菌中的选择标记

    公开(公告)号：US08268594B2

    公开(公告)日：2012-09-18

    申请号：US12083088

    申请日：2006-10-04

    申请人： Franck Martin ,  Stefano Cencioni ,  Antonella Colagrande ,  Maria Cristina Thaller ,  Marco Maria D'Andrea ,  Gian Maria Rossolini

    发明人： Franck Martin ,  Stefano Cencioni ,  Antonella Colagrande ,  Maria Cristina Thaller ,  Marco Maria D'Andrea ,  Gian Maria Rossolini

    IPC分类号： C12P21/04 ,  C12P21/06 ,  C12N1/12 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N15/70

    摘要： The present invention relates to a method of producing a recombinant protein comprising using a selection method other than antibiotics. In particular, it relates to a stable host/vector system based on the pyrC gene complementation designed to produce high level of heterologous recombinant protein in Escherichia coli. The expression system of the present invention allows rapid selection of plasmid containing cells during the cloning phases and lead to high protein expression during fermentation. This system has a strong selective efficiency, especially during the induction phase, leading to the selection of an almost homogeneous and stable plasmid bearing cell population. Moreover the productivity of the culture is to a large extent better than the one based on antibiotic resistance. This elevated vector stability combined with its high productivity fulfils the requirements for heterologous protein production in Escherichia coli to an industrial level.

    摘要翻译： 本发明涉及一种生产重组蛋白质的方法，其包括使用抗生素以外的选择方法。 特别涉及基于设计用于在大肠杆菌中产生高水平的异源重组蛋白的pyrC基因互补的稳定的宿主/载体系统。 本发明的表达系统允许在克隆阶段快速选择含有质粒的细胞，并在发酵期间导致高蛋白质表达。 该系统具有很强的选择效率，特别是在诱导阶段，导致选择几乎均匀且稳定的携带细胞的质粒。 此外，文化的生产力在很大程度上优于基于抗生素耐药性的生产力。 这种提高的载体稳定性以及高生产率满足了将大肠杆菌中异源蛋白质生产提高到工业水平的要求。

    Novel Silane Compounds and Their Use in Functionalizing Solid Supports and Immobilizing Biological Molecules on These Supports
    6.
    发明申请
    Novel Silane Compounds and Their Use in Functionalizing Solid Supports and Immobilizing Biological Molecules on These Supports 有权
    新型硅烷化合物及其在功能化固体支持物和固定生物分子在这些支持物上的用途

    公开(公告)号：US20090005269A1

    公开(公告)日：2009-01-01

    申请号：US12162799

    申请日：2007-02-01

    申请人： Franck Martin ,  Michel Granier ,  Gerard Lanneau

    发明人： Franck Martin ,  Michel Granier ,  Gerard Lanneau

    IPC分类号： C40B50/18 ,  C07F7/02 ,  C07F7/10 ,  C07F7/08

    CPC分类号： C07F7/0896 ,  C07B2200/11 ,  C07F7/12 ,  C07F7/1804

    摘要： The invention relates to novel silane compounds corresponding to the following formula (I): A-E-X  (I) in which: X represents a silylated group which makes possible the covalent attachment of the silane compound to the hydroxyl or hydride functional groups of a support; E represents an organic spacer group; A represents a group capable of releasing an —OH functional group by acid hydrolysis, the said —OH functional group, after the said hydrolysis, being covalently bonded to E. Use of these silane compounds for functionalizing solid supports and for immobilizing biological molecules on these supports.

    摘要翻译： 本发明涉及对应于下式（I）的新型硅烷化合物：<？in-line-formula description =“In-line formula”end =“lead”？> AEX（I）<？in-line-formula description =“在线式”末端=“尾”→其中：X表示使硅烷化合物与载体的羟基或氢化物官能团共价连接成为可能的甲硅烷基; E表示有机间隔基; A表示能够通过酸水解释放-OH官能团的基团，所述-OH官能团在所述水解之后共价键合到E上。这些硅烷化合物用于官能化固体载体和用于在这些上固定生物分子 支持。

    Silane compounds and use of same for functionalizing solid supports and immobilizing biological molecules on these supports
    10.
    发明授权
    Silane compounds and use of same for functionalizing solid supports and immobilizing biological molecules on these supports 有权

    公开(公告)号：US09657042B2

    公开(公告)日：2017-05-23

    申请号：US12162793

    申请日：2007-02-01

    申请人： Franck Martin

    发明人： Franck Martin

    IPC分类号： C07F7/02 ,  C07F9/40 ,  C07F7/08 ,  C07F7/12 ,  C07F7/18

    CPC分类号： C07F9/4053 ,  C07B2200/11 ,  C07F7/0896 ,  C07F7/12 ,  C07F7/1804 ,  C07F9/409

    摘要： The invention relates to novel silane compounds corresponding to the formula (I) below: A-E-X   (I) in which: X represents a silyl group capable of creating a covalent bond after reaction with the hydroxyl or hydride functional groups of a support; E represents an organic spacer group; A represents a group chosen from the groups of formulae below: in which: Z1 to Z5 independently represent a hydrogen atom or a halogen atom; Z6 and Z7 represent a group for protecting the phosphonic acid functional group, a hydrogen atom or a monovalent cation; Z8 to Z12 independently represent a group for protecting the carboxylic acid functional group, a hydrogen atom or a monovalent cation; and Z13 represents an imidazole, N-hydroxysuccinimide, nitrophenyl, pentafluorophenyl or acid anhydride group. Use of these silane compounds for functionalizing solid supports and for immobilizing biological molecules on these supports.