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    Mutated 6-phosphogluconate dehydrogenase
    1.
    发明申请
    Mutated 6-phosphogluconate dehydrogenase 有权
    突变的6-磷酸葡萄糖酸脱氢酶

    公开(公告)号：US20050079586A1

    公开(公告)日：2005-04-14

    申请号：US10497502

    申请日：2002-12-03

    申请人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

    发明人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

    IPC分类号： C12N1/21 ,  C12N9/04 ,  C12N15/53 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/22 ,  C07H21/04 ,  C12N15/74

    CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/227

    摘要： The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

    摘要翻译： 本发明涉及一种多肽，其具有衍生自属于棒杆菌属的微生物的6-磷酸葡糖酸脱氢酶（以下简写为GND）的修饰氨基酸序列，所述修饰是将位点（ s）对应于SEQ ID NO：1所示氨基酸序列的第158位和/或第361位氨基酸，并具有GND活性; 编码该多肽的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 在染色体上携带DNA的微生物; 以及生产有用物质的方法，包括在培养基中培养转化体或微生物。

    Mutated 6-phosphogluconate dehydrogenase
    2.
    发明授权
    Mutated 6-phosphogluconate dehydrogenase 有权
    突变的6-磷酸葡萄糖酸脱氢酶

    公开(公告)号：US07524667B2

    公开(公告)日：2009-04-28

    申请号：US10497502

    申请日：2002-12-03

    申请人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

    发明人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

    IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/00 ,  C12Q1/68 ,  C12P21/04 ,  C07H21/04

    CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/227

    摘要： The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

    摘要翻译： 本发明涉及一种多肽，其具有衍生自属于棒杆菌属的微生物的6-磷酸葡糖酸脱氢酶（以下简写为GND）的修饰氨基酸序列，所述修饰是将位点（ s）对应于SEQ ID NO：1所示氨基酸序列的第158位和/或第361位氨基酸，并具有GND活性; 编码多肽的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 在染色体上携带DNA的微生物; 以及生产有用物质的方法，包括在培养基中培养转化体或微生物。

    Mutant 6-phosphogluconate dehydrogenase
    3.
    发明授权
    Mutant 6-phosphogluconate dehydrogenase 有权
    突变体6-磷酸葡萄糖酸脱氢酶

    公开(公告)号：US07989191B2

    公开(公告)日：2011-08-02

    申请号：US12430592

    申请日：2009-04-27

    申请人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

    发明人： Masato Ikeda ,  Junko Ohnishi ,  Satoshi Mitsuhashi ,  Keiko Ochiai

    IPC分类号： C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12P13/04 ,  C12P21/04 ,  C12Q1/00 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/227

    摘要： The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.

    摘要翻译： 本发明涉及一种多肽，其具有衍生自属于棒杆菌属的微生物的6-磷酸葡糖酸脱氢酶（以下简写为GND）的修饰氨基酸序列，所述修饰是将位点（ s）对应于SEQ ID NO：1所示氨基酸序列的第158位和/或第361位氨基酸，并具有GND活性; 编码多肽的DNA; 包含DNA的重组DNA; 携带重组DNA的转化体; 在染色体上携带DNA的微生物; 以及生产有用物质的方法，包括在培养基中培养转化体或微生物。

    Process for producing useful substance
    8.
    发明授权
    Process for producing useful substance 有权
    生产有用物质的方法

    公开(公告)号：US08530203B2

    公开(公告)日：2013-09-10

    申请号：US13061551

    申请日：2009-08-26

    申请人： Masato Ikeda ,  Seiki Takeno ,  Yuta Mizuno ,  Satoshi Mitsuhashi

    发明人： Masato Ikeda ,  Seiki Takeno ,  Yuta Mizuno ,  Satoshi Mitsuhashi

    IPC分类号： C12P13/04

    CPC分类号： C12P13/04 ,  C07K14/34 ,  C12P13/08 ,  C12R1/15

    摘要： The present invention provides a process for efficiently producing a useful substance; and a microorganism which belongs to coryneform bacteria and which can be used in the process. The present invention provides a process for producing a useful substance by using a microorganism belonging to coryneform bacteria, the microorganism having an ability to take a sugar, which is taken into a cell via a phosphotransferase system (PTS), into a cell via a system other than the PTS, and having an ability to produce a useful substance.

    摘要翻译： 本发明提供一种有效生产有用物质的方法; 和属于棒状细菌的微生物，可用于该过程。 本发明提供了一种通过使用属于棒状细菌的微生物来生产有用物质的方法，所述微生物具有将通过磷酸转移酶系统（PTS）摄入细胞的糖的能力通过系统进入细胞 而不是PTS，并且具有产生有用物质的能力。

    PROCESS FOR PRODUCING USEFUL SUBSTANCE
    9.
    发明申请
    PROCESS FOR PRODUCING USEFUL SUBSTANCE 审中-公开
    生产有用物质的方法

    公开(公告)号：US20120034669A1

    公开(公告)日：2012-02-09

    申请号：US13201806

    申请日：2010-02-17

    申请人： Masato Ikeda ,  Seiki Takeno ,  Satoshi Mitsuhashi

    发明人： Masato Ikeda ,  Seiki Takeno ,  Satoshi Mitsuhashi

    IPC分类号： C12P1/04

    CPC分类号： C07K14/34 ,  C12P1/04 ,  C12P13/08

    摘要： The present invention provides a process for producing a useful substance which comprises culturing a transformant obtained by transforming a microorganism capable of producing the useful substance with a DNA encoding a protein in a medium, wherein the protein has an activity to improve growth of a high concentration oxygen-requiring microorganism under low oxygen concentration; forming and accumulating the useful substance in a culture; and collecting the useful substance from the culture.

    摘要翻译： 本发明提供一种生产有用物质的方法，该方法包括培养通过用培养基中编码蛋白质的DNA转化能够产生有用物质的微生物获得的转化体，其中该蛋白质具有改善高浓度生长的活性 低氧浓度下需氧微生物; 在文化中形成和积累有用物质; 并从文化中收集有用的物质。

    Process for producing L-arginine, L-ornithine or L-citrulline
    10.
    发明授权
    Process for producing L-arginine, L-ornithine or L-citrulline 有权
    生产L-精氨酸，L-鸟氨酸或L-瓜氨酸的方法

    公开(公告)号：US07741081B2

    公开(公告)日：2010-06-22

    申请号：US11575805

    申请日：2005-09-28

    申请人： Masato Ikeda ,  Tetsuo Nakano ,  Satoshi Mitsuhashi ,  Mikiro Hayashi ,  Kenji Tanaka

    发明人： Masato Ikeda ,  Tetsuo Nakano ,  Satoshi Mitsuhashi ,  Mikiro Hayashi ,  Kenji Tanaka

    IPC分类号： C12P13/10 ,  C12N9/12 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P13/10 ,  C12N9/1217 ,  C12Y207/02008

    摘要： The present invention provides a polypeptide which has: (i) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1; or (ii) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positions 20 to 38 from the N terminus of the amino acid sequence shown in SEQ ID NO: 1 and one or more amino acid residues are deleted, substituted or added in the region at positions 1 to 19 or 39 to 294; and which has N-acetylglutamate kinase activity.

    摘要翻译： 本发明提供了一种多肽，其具有：（i）氨基酸序列，其中一个或多个氨基酸残基在SEQ ID NO：1所示的氨基酸序列的N末端的位置20至38的区域中被取代; 或（ii）其中一个或多个氨基酸残基在SEQ ID NO：1所示的氨基酸序列的N末端的位置20至38的区域中被取代并且一个或多个氨基酸残基被删除的氨基酸序列 在第1至19或39至294位的区域中被取代或添加; 并具有N-乙酰谷氨酸激酶活性。