公开(公告)号：US20200224144A1

公开(公告)日：2020-07-16

申请号：US16499780

申请日：2018-03-30

Applicant: Massachusetts Institute of Technology ,  Rensselaer Polytechnic Institute

Inventor： J. Christopher Love ,  Kerry R. Love ,  Laura Crowell ,  Alan Stockdale ,  Richard Dean Braatz ,  Amos Enshen Lu ,  Steven Cramer ,  Steven Timmick ,  Nicholas Vecchiarello ,  Chaz Goodwine ,  Craig A. Mascarenhas

IPC: C12M1/00 ,  C12M1/34

Abstract: Aspects of the present disclosure relate to systems and methods for manufacturing biologically-produced pharmaceutical products. Some of the systems described herein comprise an upstream component comprising a bioreactor and at least one filter (e.g., a filter probe) integrated with a downstream component comprising a purification module comprising at least a first partitioning unit and a second partitioning unit. In some embodiments, these integrated biomanufacturing systems may be operated under continuous or conditions and may be capable of efficiently producing pure, high-quality pharmaceutical products.

公开(公告)号：US20230053902A1

公开(公告)日：2023-02-23

申请号：US17690876

申请日：2022-03-09

Applicant: Massachusetts Institute of Technology ,  Rensselaer Polytechnic Institute

Inventor： J. Christopher Love ,  Kerry R. Love ,  Laura Crowell ,  Alan Stockdale ,  Richard Dean Braatz ,  Amos Enshen Lu ,  Steven Cramer ,  Steven Timmick ,  Nicholas Vecchiarello ,  Chaz Goodwine ,  Craig A. Mascarenhas

IPC: C12M1/00 ,  C12M1/34 ,  C07K1/20 ,  C07K16/00 ,  G16C20/20

Abstract: Aspects of the present disclosure relate to systems and methods for manufacturing biologically-produced pharmaceutical products. Some of the systems described herein comprise an upstream component comprising a bioreactor and at least one filter (e.g., a filter probe) integrated with a downstream component comprising a purification module comprising at least a first partitioning unit and a second partitioning unit. In some embodiments; these integrated biomanufacturing systems may be operated under continuous or conditions and may be capable of efficiently producing pure, high-quality pharmaceutical products.

公开(公告)号：US10078778B2

公开(公告)日：2018-09-18

申请号：US14997439

申请日：2016-01-15

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G01N15/14 ,  G01N35/00 ,  G02B21/16 ,  H04N5/225 ,  G02B21/26 ,  B01L3/00 ,  G06K9/00 ,  G01N33/483 ,  G06K9/03 ,  G06K9/46 ,  G01N15/00 ,  G01N15/10

CPC classification number: G06K9/00147 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2300/12 ,  G01N15/1425 ,  G01N15/1463 ,  G01N33/4833 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2035/00148 ,  G02B21/16 ,  G02B21/26 ,  G06K9/0014 ,  G06K9/036 ,  G06K9/4647 ,  G06K9/4652 ,  G06K9/4661 ,  H04N5/2256

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US20200251186A1

公开(公告)日：2020-08-06

申请号：US16499776

申请日：2018-03-30

Applicant: Massachusetts Institute of Technology ,  Rensselaer Polytechnic Institute

Inventor： J. Christopher Love ,  Kerry R. Love ,  Steven Cramer ,  Steven Timmick ,  Nicholas Vecchiarello ,  Chaz Goodwine ,  Laura Crowell ,  Alan Stockdale ,  Richard Dean Braatz ,  Amos Enshen Lu

IPC: G16C20/10 ,  C07K1/20 ,  G16C20/70 ,  C07K14/535 ,  C07K14/61 ,  C07K14/56 ,  C07K16/00

Abstract: Systems and methods for generating and evaluating candidate sequences of partitioning steps to partition at least one biologically produced product from at least one impurity. In some embodiments, a plurality of candidate sequences of partitioning steps may be generated, wherein at least one candidate sequence of the plurality of candidate sequences comprises a plurality of partitioning steps in a specified order. The plurality of candidate sequences may be evaluated. For instance, a data set associated with the at least one partitioning step may be accessed, the data set comprising: first data indicative of a behavior of the at least one biologically produced product with respect to the at least one partitioning step; and second data indicative of a behavior of the at least one impurity with respect to the at least one partitioning step. The at least one candidate sequence may be scored based at least in part on the data set.

公开(公告)号：US20190005304A1

公开(公告)日：2019-01-03

申请号：US16102118

申请日：2018-08-13

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G06K9/00 ,  G01N33/483 ,  G01N15/14 ,  G01N35/00

CPC classification number: G06K9/00147 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2300/12 ,  G01N15/1425 ,  G01N15/1463 ,  G01N33/4833 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2035/00148 ,  G02B21/16 ,  G02B21/26 ,  G06K9/0014 ,  G06K9/036 ,  G06K9/4647 ,  G06K9/4652 ,  G06K9/4661 ,  H04N5/2256

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US12172166B2

公开(公告)日：2024-12-24

申请号：US16990390

申请日：2020-08-11

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G01N15/01 ,  B01L3/00 ,  G01B21/08 ,  G01N15/14 ,  G01N15/1433 ,  G01N33/483 ,  G01N35/00 ,  G02B21/00 ,  G02B21/06 ,  G02B21/16 ,  G02B21/26 ,  G06V20/69 ,  H04N23/56 ,  G01N15/10

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US20200372241A1

公开(公告)日：2020-11-26

申请号：US16990390

申请日：2020-08-11

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G06K9/00 ,  B01L3/00 ,  G01N15/14 ,  G01N35/00 ,  G02B21/16 ,  G02B21/26 ,  H04N5/225 ,  G01N33/483 ,  G06K9/03 ,  G06K9/46

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US10776608B2

公开(公告)日：2020-09-15

申请号：US16102118

申请日：2018-08-13

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G06K9/00 ,  B01L3/00 ,  G01N15/14 ,  G01N35/00 ,  G02B21/16 ,  G02B21/26 ,  H04N5/225 ,  G01N33/483 ,  G06K9/03 ,  G06K9/46 ,  G01N15/00 ,  G01N15/10

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US09953209B2

公开(公告)日：2018-04-24

申请号：US15085175

申请日：2016-03-30

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G06K9/00 ,  B01L3/00 ,  G01N15/14 ,  G01N35/00 ,  G02B21/16 ,  G02B21/26 ,  H04N5/225 ,  G01N33/483 ,  G06K9/03 ,  G06K9/46 ,  G01N15/00 ,  G01N15/10

CPC classification number: G06K9/00147 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2300/12 ,  G01N15/1425 ,  G01N15/1463 ,  G01N33/4833 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2035/00148 ,  G02B21/16 ,  G02B21/26 ,  G06K9/0014 ,  G06K9/036 ,  G06K9/4647 ,  G06K9/4652 ,  G06K9/4661 ,  H04N5/2256

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

公开(公告)号：US20160217315A1

公开(公告)日：2016-07-28

申请号：US15085175

申请日：2016-03-30

Applicant: Massachusetts Institute of Technology

Inventor： Viktor A. Adalsteinsson ,  Denis Loginov ,  J. Christopher Love ,  Alan Stockdale ,  Todd Gierahn

IPC: G06K9/00 ,  G06K9/46 ,  G06K9/03 ,  G01N33/483

CPC classification number: G06K9/00147 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2300/12 ,  G01N15/1425 ,  G01N15/1463 ,  G01N33/4833 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2035/00148 ,  G02B21/16 ,  G02B21/26 ,  G06K9/0014 ,  G06K9/036 ,  G06K9/4647 ,  G06K9/4652 ,  G06K9/4661 ,  H04N5/2256

Abstract: Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.