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    Nucleic Acid Amplification and Sequencing on a Droplet Actuator
    1.
    发明申请
    Nucleic Acid Amplification and Sequencing on a Droplet Actuator 审中-公开
    滴液致动器中的核酸扩增和测序

    公开(公告)号：US20110311980A1

    公开(公告)日：2011-12-22

    申请号：US13139467

    申请日：2009-12-15

    申请人： Michael G. Pollack ,  Allen E. Eckhardt ,  Vijay Srinivasan ,  Prasanna Thwar

    发明人： Michael G. Pollack ,  Allen E. Eckhardt ,  Vijay Srinivasan ,  Prasanna Thwar

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/10 ,  B01L2200/143 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0644 ,  C12Q1/6869 ,  C12Q2565/301 ,  C12Q2565/626

    摘要： The invention provides a droplet actuator device, as well as systems, methods and devices making use of the droplet actuator device. The droplet actuator device may include a substrate having electrodes arranged for conducting one or more droplet operations. The droplet actuator device may include a substrate having a reactor path with a wash region associated with a magnet for immobilizing mobilizing beads during bead washing operations. The droplet actuator device may include nucleotide base reservoirs and dedicated nucleotide base electrode paths arranged for transporting nucleotide base droplets from nucleotide base reservoirs to the reactor path. The droplet actuator device may include one or more wash buffer reservoirs associated with electrode paths arranged for transporting wash buffer droplets from wash buffer reservoirs to the reactor path. The droplet actuator device may include one or more sample reservoirs and sample paths arranged for transporting sample droplets from the one or more sample reservoirs to the reactor path. The droplet actuator device may include one or more enzyme reservoirs and dedicated enzyme electrode paths arranged for transporting enzyme droplets from the one or more enzyme reservoirs to a detection electrode.

    摘要翻译： 本发明提供一种液滴致动器装置，以及利用液滴致动器装置的系统，方法和装置。 液滴致动器装置可以包括具有布置用于进行一个或多个液滴操作的电极的基板。 液滴致动器装置可以包括具有反应器路径的基底，其具有与磁珠相关联的洗涤区域，用于在珠洗涤操作期间固定动员珠粒。 液滴致动器装置可以包括核苷酸碱基储存器和专用核苷酸碱基电极路径，其设置用于将核苷酸碱基液滴从核苷酸碱基储存器输送到反应器路径。 液滴致动器装置可以包括一个或多个洗涤缓冲液储存器，其与设置用于​​将洗涤缓冲液滴从洗涤缓冲液储存器运送到反应器路径的电极路径相关联。 液滴致动器装置可以包括一个或多个样品储存器和样品路径，其被布置用于将样品液滴从一个或多个样品储存器输送到反应器路径。 液滴致动器装置可以包括一个或多个酶储器和用于将酶液滴从一个或多个酶储存器输送到检测电极的专用酶电极路径。

    Method of Detecting an Analyte
    5.
    发明申请
    Method of Detecting an Analyte 有权
    检测分析物的方法

    公开(公告)号：US20090311713A1

    公开(公告)日：2009-12-17

    申请号：US12546253

    申请日：2009-08-24

    申请人： Michael G. Pollack ,  Zhishan Hua ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Vijay Srinivasan ,  Vamsee K. Pamula

    发明人： Michael G. Pollack ,  Zhishan Hua ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Vijay Srinivasan ,  Vamsee K. Pamula

    IPC分类号： C12Q1/68

    CPC分类号： B01L3/502792 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/043 ,  C12Q1/6848 ,  C12Q2565/629

    摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, a method of detecting an analyte is provided and includes providing in a detection window a droplet including a signal-producing substance indicative of the presence and/or quantity of an analyte and one or more magnetically responsive beads which may interfere with signal produced by the signal producing substance. The method further includes using a magnetic field for magnetically removing the magnetically responsive beads from the detection window and/or magnetically restricting the magnetically responsive beads from entering the detection window while transporting and/or retaining the droplet in the detection window. The method additionally includes detecting a signal produced by the signal-producing substance.

    摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，提供检测分析物的方法，并且包括在检测窗口中提供包含指示分析物的存在和/或量的信号产生物质的液滴和一个或多个可能干扰信号的磁响应珠子 由信号产生物质产生。 该方法还包括使用磁场来从检测窗口磁性地去除磁响应珠子和/或磁力限制磁响应珠子进入检测窗口，同时在检测窗口中传送和/或保持液滴。 该方法还包括检测由信号产生物质产生的信号。

    Method of Ligating a Nucleic Acid
    8.
    发明申请
    Method of Ligating a Nucleic Acid 审中-公开
    核酸的方法

    公开(公告)号：US20130225450A1

    公开(公告)日：2013-08-29

    申请号：US13596548

    申请日：2012-08-28

    申请人： Michael G. Pollack ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Jeremy Rouse

    发明人： Michael G. Pollack ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Jeremy Rouse

    IPC分类号： B01J19/00

    CPC分类号： C12N15/1075 ,  B01J19/0046 ,  C12N15/1006 ,  C40B40/08 ,  C40B50/06

    摘要： A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.

    摘要翻译： 制备与油接触的液滴核酸文库的方法，包括：（a）在油中的液滴中的平端核酸片段以产生平端核酸片段; （b）使油中的液滴中的平端核酸片段磷酸化以产生磷酸化的核酸片段; 将A尾连接到油中的液滴中的磷酸化核酸片段以产生A尾核酸片段; 和（d）将核酸衔接子偶联到油中的液滴中的A尾核酸片段，以产生包含衔接子连接的核酸片段的核酸文库。