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公开(公告)号:US09675972B2
公开(公告)日:2017-06-13
申请号:US12921256
申请日:2009-03-09
申请人: Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人: Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
CPC分类号: B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
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公开(公告)号:US20110104747A1
公开(公告)日:2011-05-05
申请号:US12921256
申请日:2009-03-09
申请人: Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人: Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
IPC分类号: G01N1/40
CPC分类号: B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
摘要翻译: 提供了将珠子集中在流体装置上的液滴和/或负载珠粒中的方法,其中包括将珠粒集中在液滴中的方法,所述方法包括:(a)提供液滴致动器,其包括:(i) 内部液滴操作量; 和(ii)内部容积外部的储存器; (iii)建立在从储存器延伸到内部容积中的液体路径中的液滴; (b)在储存器中的液滴部分中提供磁响应珠; (c)通过所述液体路径将所述磁响应珠磁吸引到位于所述内部容积中的所述液滴的所述部分中; 和(d)在内部容积中形成包含一个或多个磁响应珠的液滴。
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公开(公告)号:US08852952B2
公开(公告)日:2014-10-07
申请号:US12990815
申请日:2009-05-04
申请人: Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Allen Eckhardt , Alexander Shenderov , Dwayne Allen , Vijay Srinivasan
发明人: Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Allen Eckhardt , Alexander Shenderov , Dwayne Allen , Vijay Srinivasan
IPC分类号: G01N1/00
CPC分类号: B01L3/502784 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2300/022 , B01L2300/023 , B01L2300/024 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , G01N27/44704 , G01N35/0098 , G01N2035/1046 , Y10T436/25 , Y10T436/2575
摘要: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.
摘要翻译: 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒,以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。
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公开(公告)号:US20110104816A1
公开(公告)日:2011-05-05
申请号:US12990815
申请日:2009-05-04
申请人: Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Vijay Srinivasan , Allen Eckhardt , Alexander Shenderov , Dwayne Allen
发明人: Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Vijay Srinivasan , Allen Eckhardt , Alexander Shenderov , Dwayne Allen
IPC分类号: G01N1/00
CPC分类号: B01L3/502784 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2300/022 , B01L2300/023 , B01L2300/024 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , G01N27/44704 , G01N35/0098 , G01N2035/1046 , Y10T436/25 , Y10T436/2575
摘要: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.
摘要翻译: 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒,以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。
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公开(公告)号:US08591830B2
公开(公告)日:2013-11-26
申请号:US12673893
申请日:2008-08-25
IPC分类号: B01L3/00
CPC分类号: B01L3/52 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2300/0864 , B01L2300/089 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427
摘要: A droplet actuator comprising: (a) a base substrate comprising electrodes configured for conducting droplet operations on a droplet operations surface thereof; (b) a droplet comprising one or more beads situated on the droplet operations surface; (c) a barrier arranged in relation to the droplet and the electrodes such that a droplet may be transported away from the beads using one or more droplet operations mediated by one or more of the electrodes while transport of the beads is restrained by a barrier. Related methods and kits are also provided.
摘要翻译: 一种液滴致动器,包括:(a)基底基底,包括构造成用于在其液滴操作表面上进行液滴操作的电极; (b)包含位于所述液滴操作表面上的一个或多个珠粒的液滴; (c)相对于液滴和电极布置的屏障,使得可以使用由一个或多个电极介导的一个或多个液滴操作将液滴从珠流出,同时珠的传送被屏障限制。 还提供了相关方法和试剂盒。
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公开(公告)号:US20110303542A1
公开(公告)日:2011-12-15
申请号:US12672564
申请日:2008-08-08
IPC分类号: G01N27/447 , B01D57/02
CPC分类号: B01L3/502792 , B01L2200/141 , B01L2300/0645 , B01L2400/0415 , G01N2035/00277
摘要: The invention relates to a droplet actuator with a substrate comprising electrodes arranged for conducting droplet operations on a droplet operations surface of the substrate; a filler fluid phase in contact with the droplet operations surface at least partially surrounding a droplet phase comprising a droplet arranged on one or more of the electrodes, the droplet comprising: (i) a target substance susceptible to loss from the droplet phase into the filler fluid phase; and (ii) an additive which reduces loss of the target substance to the filler fluid phase relative to a corresponding droplet not comprising the additive. The invention also relates to various compositions and methods.
摘要翻译: 本发明涉及一种液滴致动器,其具有包括布置用于在基板的液滴操作表面上进行液滴操作的电极的基板; 与液滴操作表面接触的填充流体相,至少部分地围绕包含布置在一个或多个电极上的液滴的液滴相,所述液滴包括:(i)易受从液滴相流入填料中的目标物质 流体相; 和(ii)相对于不包含添加剂的相应液滴,减少目标物质对填料流体相的损失的添加剂。 本发明还涉及各种组合物和方法。
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公开(公告)号:US20110086377A1
公开(公告)日:2011-04-14
申请号:US12673893
申请日:2008-08-25
申请人: Prasanna Thwar , Dwayne Allen , Philip Paik
发明人: Prasanna Thwar , Dwayne Allen , Philip Paik
CPC分类号: B01L3/52 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2300/0864 , B01L2300/089 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427
摘要: A droplet actuator comprising: (a) a base substrate comprising electrodes configured for conducting droplet operations on a droplet operations surface thereof; (b) a droplet comprising one or more beads situated on the droplet operations surface; (c) a barrier arranged in relation to the droplet and the electrodes such that a droplet may be transported away from the beads using one or more droplet operations mediated by one or more of the electrodes while transport of the beads is restrained by a barrier. Related methods and kits are also provided.
摘要翻译: 一种液滴致动器,包括:(a)基底基底,包括构造成用于在其液滴操作表面上进行液滴操作的电极; (b)包含位于所述液滴操作表面上的一个或多个珠粒的液滴; (c)相对于液滴和电极布置的屏障,使得可以使用由一个或多个电极介导的一个或多个液滴操作将液滴从珠流出,同时珠的传送被屏障限制。 还提供了相关方法和试剂盒。
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