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19 条记录 (耗时 0.033 秒)

    Method of conducting a droplet based enzymatic assay
    8.
    发明授权
    Method of conducting a droplet based enzymatic assay 有权
    进行基于液滴的酶法测定的方法

    公开(公告)号：US08440392B2

    公开(公告)日：2013-05-14

    申请号：US12531844

    申请日：2008-03-23

    申请人： Vamsee K. Pamula ,  Allen Eckhardt ,  Jeremy Rouse ,  Vijay Srinivasan

    发明人： Vamsee K. Pamula ,  Allen Eckhardt ,  Jeremy Rouse ,  Vijay Srinivasan

    IPC分类号： C12Q1/00

    CPC分类号： G01N21/6486 ,  B01F13/0809 ,  C07H15/04 ,  C12Q1/34 ,  G01N2333/928 ,  G01N2800/042

    摘要： A method of conducting a droplet-based enzymatic assay is provided. The method generally makes use of a droplet actuator. A droplet comprising an enzyme of interest is provided on the droplet actuator along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. The method involves executing droplet operations on the droplet actuator to combine the droplets, thereby yielding an assay droplet, and detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator. The enzyme of interest may, for example, be a potentially mutated or improperly folded enzyme exhibiting altered enzyme activity as compared to a corresponding normal enzyme.

    摘要翻译： 提供了一种进行基于液滴的酶测定的方法。 该方法通常使用液滴致动器。 包含感兴趣的酶的液滴与包含底物的液滴一起提供在液滴致动器上，所述液滴在酶的存在下可能被修饰。 该方法涉及对液滴致动器执行液滴操作以组合液滴，从而产生测定液滴，并且通过液滴致动器上的测定液滴中的酶检测底物的修饰。 感兴趣的酶可以是例如与相应的正常酶相比显示改变的酶活性的潜在突变或不正确折叠的酶。

    Integrated droplet actuator for gel; electrophoresis and molecular analysis
    10.
    发明授权
    Integrated droplet actuator for gel; electrophoresis and molecular analysis 有权
    凝胶液滴致动器 电泳和分子分析

    公开(公告)号：US09091649B2

    公开(公告)日：2015-07-28

    申请号：US13508300

    申请日：2010-11-08

    申请人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    发明人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    IPC分类号： G01N27/447 ,  B01L7/00 ,  B01L3/00

    CPC分类号： G01N27/44743 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/046 ,  G01N27/44791

    摘要： The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

    摘要翻译： 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术，液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶，捕获并随后处理电泳凝胶的产物，或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中，在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定，例如通过实时PCR的核酸（例如DNA）定量和一个或多个样品处理操作，例如样品稀释。 在一个实施方案中，电泳凝胶可以集成在液滴致动器的顶部基底上。