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公开(公告)号：US09675972B2
公开(公告)日：2017-06-13
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
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公开(公告)号：US20110104747A1
公开(公告)日：2011-05-05
申请号：US12921256
申请日：2009-03-09
申请人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
发明人： Michael G. Pollack , Ramakrishna Sista , Zhishan Hua , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Prasanna Thwar , Alexander Shenderov , Dwayne Allen
IPC分类号： G01N1/40
CPC分类号： B01L3/0282 , B01F13/002 , B01F13/0023 , B01F13/0071 , B01F13/0076 , B01F13/0818 , B01L3/502753 , B01L2200/0621 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2200/141 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2400/043 , B03C1/01 , B03C1/284 , B03C1/286 , B03C2201/18 , B03C2201/26 , B03C2201/28 , Y10T436/25
摘要： Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.
摘要翻译： 提供了将珠子集中在流体装置上的液滴和/或负载珠粒中的方法，其中包括将珠粒集中在液滴中的方法，所述方法包括：（a）提供液滴致动器，其包括：（i） 内部液滴操作量; 和（ii）内部容积外部的储存器; （iii）建立在从储存器延伸到内部容积中的液体路径中的液滴; （b）在储存器中的液滴部分中提供磁响应珠; （c）通过所述液体路径将所述磁响应珠磁吸引到位于所述内部容积中的所述液滴的所述部分中; 和（d）在内部容积中形成包含一个或多个磁响应珠的液滴。
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公开(公告)号：US20110256542A9
公开(公告)日：2011-10-20
申请号：US12546115
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：US20090311713A1
公开(公告)日：2009-12-17
申请号：US12546253
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, a method of detecting an analyte is provided and includes providing in a detection window a droplet including a signal-producing substance indicative of the presence and/or quantity of an analyte and one or more magnetically responsive beads which may interfere with signal produced by the signal producing substance. The method further includes using a magnetic field for magnetically removing the magnetically responsive beads from the detection window and/or magnetically restricting the magnetically responsive beads from entering the detection window while transporting and/or retaining the droplet in the detection window. The method additionally includes detecting a signal produced by the signal-producing substance.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，提供检测分析物的方法，并且包括在检测窗口中提供包含指示分析物的存在和/或量的信号产生物质的液滴和一个或多个可能干扰信号的磁响应珠子 由信号产生物质产生。 该方法还包括使用磁场来从检测窗口磁性地去除磁响应珠子和/或磁力限制磁响应珠子进入检测窗口，同时在检测窗口中传送和/或保持液滴。 该方法还包括检测由信号产生物质产生的信号。
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公开(公告)号：US20110097763A1
公开(公告)日：2011-04-28
申请号：US12991966
申请日：2009-05-13
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12P19/34
CPC分类号： B01L7/525 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L2200/027 , B01L2200/0668 , B01L2200/10 , B01L2300/0803 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/088 , B01L2300/0883 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/043 , C12Q1/686 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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6.发明申请Droplet Actuator Configurations and Methods of Conducting Droplet Operations 审中-公开液滴执行器配置和执行液滴操作的方法公开(公告)号：US20100270156A1
公开(公告)日：2010-10-28
申请号：US12682830
申请日：2008-12-23
申请人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Paik
发明人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Paik
IPC分类号： C07K1/26
CPC分类号： B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
摘要： A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
摘要翻译： 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。
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公开(公告)号：US20100221713A1
公开(公告)日：2010-09-02
申请号：US12546115
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：US08562807B2
公开(公告)日：2013-10-22
申请号：US12747231
申请日：2008-12-10
申请人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
发明人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
IPC分类号： G01N27/453
CPC分类号： G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
摘要： Droplet actuators for conducting droplet operations, such as droplet transport and droplet dispensing, are provided. In one embodiment, the droplet actuator may include a substrate including, droplet operations electrodes arranged for conducting droplet operations on a surface of the substrate; and reference electrodes associated with the droplet operations electrodes and extending above the surface of the substrate. Other embodiments of droplet actuators and methods of loading and using such droplet actuators are also provided.
摘要翻译： 提供用于进行液滴操作的滴液致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括基板，其包括布置成在基板的表面上进行液滴操作的液滴操作电极; 以及与液滴操作电极相关并在衬底表面上方延伸的参考电极。 还提供了液滴致动器的其他实施例以及加载和使用这种液滴致动器的方法。
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公开(公告)号：US20130288254A1
公开(公告)日：2013-10-31
申请号：US13390121
申请日：2010-08-13
申请人： Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Prasanna Thwar , Zhishan Hua , Arjun Sudarsan , Theodore Winger
发明人： Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Prasanna Thwar , Zhishan Hua , Arjun Sudarsan , Theodore Winger
IPC分类号： C12Q1/68
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L2200/0668 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/686 , G01N33/54326 , G01N2035/00425 , G01N2035/1058 , G01N2035/1062 , C12Q2549/101 , C12Q2527/15 , C12Q2527/107 , C12Q2561/113
摘要： The invention is directed to certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuator methods for detection of single nucleotide polymorphisms (SNPs) in a DNA sequence using digital microfluidics, including droplet actuator-based sample preparation and SNP analysis. In another embodiment, the invention provides droplet actuator devices and methods for providing integrated sample preparation and multiplexed detection of an infectious agent, such as HIV. In yet another embodiment, the invention provides droplet actuator devices and techniques for PCR amplification and detection of specific nucleic acid sequences using digital microfluidics, including droplet actuator-based sample preparation and target nucleic acid analysis. In yet another embodiment the invention provides methods for performing hot-start PCR on a droplet actuator. In yet another embodiment, the method of the invention combines PCR amplification with pyrosequencing to investigate specific sequences.
摘要翻译： 本发明涉及某些液滴致动分子技术。 在一个实施方案中，本发明提供用于使用数字微流体（包括基于液滴致动器的样品制备和SNP分析）检测DNA序列中的单核苷酸多态性（SNP）的液滴致动器方法。 在另一个实施方案中，本发明提供了液滴致动器装置和用于提供综合样品制备和多重检测感染因子如HIV的方法。 在另一个实施方案中，本发明提供了使用数字微流体（包括基于液滴致动器的样品制备和靶核酸分析）的PCR扩增和检测特异性核酸序列的液滴致动器装置和技术。 在另一个实施方案中，本发明提供了在液滴致动器上进行热启动PCR的方法。 在另一个实施方案中，本发明的方法将PCR扩增与焦磷酸测序结合以研究特异性序列。
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公开(公告)号：US08702938B2
公开(公告)日：2014-04-22
申请号：US12676384
申请日：2008-09-04
CPC分类号： B01L3/502792 , B01L2200/12 , B01L2300/0819 , B01L2300/089 , B01L2400/0415 , B01L2400/0421 , B01L2400/0427 , Y10T29/49826
摘要： The invention provides a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein: (a) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and (b) the top substrate comprises a first portion coupled to second portion, where the second portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.
摘要翻译： 本发明提供了一种液滴致动器，其包括分离的基底基板和顶部基板以形成间隙，其中：（a）基底基板包括构造成用于在间隙中进行液滴操作的电极; 和（b）顶部衬底包括联接到第二部分的第一部分，其中第二部分包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。
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