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    Control of materials and porous magnetic particles
    1.
    发明授权
    Control of materials and porous magnetic particles 有权
    材料和多孔磁性颗粒的控制

    公开(公告)号：US08377147B2

    公开(公告)日：2013-02-19

    申请号：US12300369

    申请日：2007-05-16

    申请人： Michael J. Sailor ,  Ji-Ho Park ,  Austin Derfus ,  Ester Segal ,  Kenneth S. Vecchio ,  Sangeeta N. Bhatia

    发明人： Michael J. Sailor ,  Ji-Ho Park ,  Austin Derfus ,  Ester Segal ,  Kenneth S. Vecchio ,  Sangeeta N. Bhatia

    IPC分类号： B32B15/00 ,  B44C1/22

    CPC分类号： H05B6/02 ,  B82Y25/00 ,  H01F1/0063 ,  Y10T428/29 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/32

    摘要： The present invention uses externally applied electromagnetic stimulus to control and heat porous magnetic particles and material associated with the particles. The particles contain magnetic material, such as superparamagnetic iron oxide and are infused with a material. Application of a DC magnetic field allows them to be moved with their infused material, and application of an AC RF electromagnetic field allows them to be heated with their infused material. The material can be infused into pores of the particles and the particles can also adhere to an aqueous droplet. The present invention also provides a multi-layer porous magnetic particle. The particle includes a host layer having pores sized to accept magnetic nanoparticles. Magnetic nanoparticles are infused within pores of the host layer. An encoding layer includes pores that define a spectral code. The pores in the encoding layer are sized to substantially exclude the magnetic nanoparticles. The encoding layer can also be a multi-layer, exhibiting, for example, a complex spectral code.

    摘要翻译： 本发明使用外部施加的电磁刺激来控制和加热与颗粒相关联的多孔磁性颗粒和材料。 颗粒包含磁性材料，例如超顺磁性氧化铁，并且注入材料。 直流磁场的应用使得它们可以通过其输入的材料移动，并且使用AC RF电磁场允许它们与其输入的材料一起被加热。 该材料可以注入到颗粒的孔中，并且颗粒也可以粘附到水滴上。 本发明还提供一种多层多孔磁性颗粒。 颗粒包括具有尺寸以接受磁性纳米颗粒的孔的主体层。 磁性纳米颗粒注入主体层的孔内。 编码层包括限定光谱代码的孔。 编码层中的孔的大小被确定为基本排除磁性纳米颗粒。 编码层也可以是多层，表现出例如复谱谱。

    CONTROL OF MATERIALS AND POROUS MAGNETIC PARTICLES
    2.
    发明申请
    CONTROL OF MATERIALS AND POROUS MAGNETIC PARTICLES 有权
    材料和多孔磁性颗粒的控制

    公开(公告)号：US20090179171A1

    公开(公告)日：2009-07-16

    申请号：US12300369

    申请日：2007-05-16

    申请人： Michael J. Sailor ,  Ji-Ho Park ,  Austin M. Derfus ,  Ester Segal ,  Kenneth S. Vecchio ,  Sangeeta N. Bhatia

    发明人： Michael J. Sailor ,  Ji-Ho Park ,  Austin M. Derfus ,  Ester Segal ,  Kenneth S. Vecchio ,  Sangeeta N. Bhatia

    IPC分类号： H01F1/00 ,  H05B6/02

    CPC分类号： H05B6/02 ,  B82Y25/00 ,  H01F1/0063 ,  Y10T428/29 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/32

    摘要： The present invention uses externally applied electromagnetic stimulus to control and heat porous magnetic particles and material associated with the particles. The particles contain magnetic material, such as superparamagnetic iron oxide and are associated with a material. Application of a DC magnetic field allows them to be moved with their associated material, and application of an AC RF electromagnetic field allows them to be heated with their associated material. The material can be associated with the particles by being contained in the pores of the particles, or in other cases the particles can adhere to the associated material, which can be an aqueous droplet. The present invention also provides a multi-layer porous magnetic particle. The particle includes a host layer having pores sized to accept magnetic nanoparticles. Magnetic nanoparticles are infused within pores of the host layer An encoding layer includes pores that define a spectral code. The pores in the encoding layer are sized to substantially exclude the magnetic nanoparticles. The encoding layer can also be a multi-layer, exhibiting, for example, a complex spectral code.

    摘要翻译： 本发明使用外部施加的电磁刺激来控制和加热与颗粒相关联的多孔磁性颗粒和材料。 颗粒含有磁性材料，例如超顺磁性氧化铁，并与材料相关联。 DC磁场的应用允许它们与其相关材料一起移动，并且AC RF电磁场的应用允许它们与其相关材料一起被加热。 该材料可以通过包含在颗粒的孔中与颗粒相关联，或者在其它情况下，颗粒可以附着到可以是水性液滴的相关材料上。 本发明还提供一种多层多孔磁性颗粒。 颗粒包括具有尺寸以接受磁性纳米颗粒的孔的主体层。 磁性纳米颗粒注入到主体层的孔内编码层包括限定光谱代码的孔。 编码层中的孔的大小被确定为基本排除磁性纳米颗粒。 编码层也可以是多层，表现出例如复谱谱。

    Method of screening compounds using a nanoporous silicon support containing macrowells for cells
    5.
    发明授权
    Method of screening compounds using a nanoporous silicon support containing macrowells for cells 失效
    使用包含用于细胞的孔眼的纳米多孔硅载体筛选化合物的方法

    公开(公告)号：US07312046B2

    公开(公告)日：2007-12-25

    申请号：US10787015

    申请日：2004-02-25

    申请人： Vicki I. Chin ,  Sangeeta N. Bhatia ,  Michael J. Sailor ,  Boyce E. Collins

    发明人： Vicki I. Chin ,  Sangeeta N. Bhatia ,  Michael J. Sailor ,  Boyce E. Collins

    IPC分类号： C12Q1/02 ,  C12Q1/18 ,  C12Q1/20 ,  C12N1/00 ,  C12N5/00 ,  C12M1/00 ,  C12M3/00

    CPC分类号： G01N33/5014 ,  C12M25/14 ,  C12M29/10 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5067 ,  G01N33/5088 ,  G01N33/552 ,  G01N2500/20

    摘要： A nanoporous silicon support comprising a plurality of macropores is provided to function as a bioreactor for the maintenance of cells in culture in a differentiated state. Each cell or group of cells is grown in an individual macropore and is provided with nutrients by means such as perfusion of the nanoporous silicon support with fluid. The macropores may be between 0.2 and 200 microns and be coated with a substance that promotes cell adhesion. The support containing cells may be used to used to test compounds for biological activity, metabolism, toxicity, mutagenicity, carcinogenicity or to characterize novel or unknown comounds. The supports are sufficiently robust that they may be assembled into larger reactors to simulate organ function or be used for the production of biomolecules.

    摘要翻译： 提供包含多个大孔的纳米多孔硅载体用作生物反应器，用于维持分化状态的培养中的细胞。 每个细胞或一组细胞在单独的大孔中生长，并通过诸如用流体灌注纳米多孔硅载体的方式提供营养。 大孔可以在0.2和200微米之间，并且涂覆有促进细胞粘附的物质。 含有细胞的载体可用于测试化合物的生物活性，代谢，毒性，致突变性，致癌性或表征新颖或未知的化合物。 载体足够坚固，可将其组装成较大的反应器以模拟器官功能或用于生产生物分子。

    Nanoporous silicon support containing macropores for use as a bioreactor
    6.
    发明授权
    Nanoporous silicon support containing macropores for use as a bioreactor 失效
    含有大孔的纳米多孔硅载体用作生物反应器

    公开(公告)号：US06734000B2

    公开(公告)日：2004-05-11

    申请号：US09981691

    申请日：2001-10-12

    申请人： Vicki I. Chin ,  Sangeeta N. Bhatia ,  Michael J. Sailor ,  Boyce E. Collins

    发明人： Vicki I. Chin ,  Sangeeta N. Bhatia ,  Michael J. Sailor ,  Boyce E. Collins

    IPC分类号： C12M1114

    CPC分类号： G01N33/5014 ,  C12M25/14 ,  C12M29/10 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5067 ,  G01N33/5088 ,  G01N33/552 ,  G01N2500/20

    摘要： A nanoporous silicon support comprising a plurality of macropores is provided to function as a bioreactor for the maintenance of cells in culture in a differentiated state. Each cell or group of cells is grown in an individual macropore and is provided with nutrients such as by perfusion of the nanoporous silicon support with fluid. The macropores may be between 0.2 and 200 microns and be coated with a substance that provides cell adhesion. The support containing cells may be used to used to test compounds for biological activity, metabolism, toxicity, mutagenicity, carcinogenicity or to characterize novel or unknown comounds. The support is sufficiently robust that it may be assembled into larger reactors to simulate organ function or be used for the production of biomolecules.

    摘要翻译： 提供包含多个大孔的纳米多孔硅载体用作生物反应器，用于维持分化状态的培养中的细胞。 每个细胞或一组细胞在单个大孔中生长并且提供营养物质，例如通过用流体灌注纳米多孔硅载体。 大孔可以在0.2至200微米之间，并且涂覆有提供细胞粘附的物质。 含有细胞的载体可用于测试化合物的生物活性，代谢，毒性，致突变性，致癌性或表征新颖或未知的化合物。 支架足够坚固，可将其装配到较大的反应器中以模拟器官功能或用于生产生物分子。

    Multi-well micropatterning by ablation
    8.
    发明申请
    Multi-well micropatterning by ablation 审中-公开
    消融多孔微图案

    公开(公告)号：US20080220516A1

    公开(公告)日：2008-09-11

    申请号：US11974341

    申请日：2007-10-12

    申请人： David T. Eddington ,  Sangeeta N. Bhatia

    发明人： David T. Eddington ,  Sangeeta N. Bhatia

    IPC分类号： C12M1/22 ,  B44C1/22

    CPC分类号： G01N33/5067 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00587 ,  B01J2219/0898 ,  B01L3/5025 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0636 ,  C12M23/12 ,  C12M23/20 ,  C12M25/04 ,  C12N5/0671 ,  C12N2502/14 ,  C12N2535/10 ,  G01N33/5008 ,  G01N33/54353 ,  G01N33/5436

    摘要： The present invention is drawn to the generation of micropatterns of biomolecules and cells on standard laboratory materials through selective ablation of a physisorbed biomolecule with oxygen plasma. In certain embodiments, oxygen plasma is able to ablate selectively physisorbed layers of biomolecules (e.g., type-I collagen, fibronectin, laminin, and Matrigel) along complex non-linear paths which are difficult or impossible to pattern using alternative methods. In addition, certain embodiments of the present invention relate to the micropatterning of multiple cell types on curved surfaces, multiwell plates, and flat bottom flasks. The invention also features kits for use with the subject methods.

    摘要翻译： 本发明涉及通过用氧等离子体选择性消融物理吸附的生物分子来生成标准实验室材料上的生物分子和细胞的微图案。 在某些实施方案中，氧等离子体能够沿着复杂的非线性路径选择性地吸收生物分子（例如，I型胶原，纤连蛋白，层粘连蛋白和Matrigel）的物理吸附层，这些复杂的非线性路径难以或不可能使用替代方法进行模式化。 此外，本发明的某些实施例涉及在曲面，多孔板和平底烧瓶上的多种细胞类型的微图案化。 本发明还具有用于本发明方法的试剂盒。

    Micromechanical devices for control of cell-cell interaction, and methods of use thereof
    10.
    发明授权
    Micromechanical devices for control of cell-cell interaction, and methods of use thereof 有权

    公开(公告)号：US09611453B2

    公开(公告)日：2017-04-04

    申请号：US12449994

    申请日：2008-03-10

    申请人： Sangeeta N. Bhatia ,  Elliot Hui

    发明人： Sangeeta N. Bhatia ,  Elliot Hui

    IPC分类号： C12Q1/02 ,  C12M1/42 ,  C12M1/12 ,  C12M1/00

    CPC分类号： C12M35/08 ,  C12M23/04 ,  C12M23/20

    摘要： The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By employing a micromachined silicon substrate with moving parts, herein is disclosed the dynamic regulation of cell-cell interactions via direct manipulation of adherent cells with micron-scale precision. The inventive devices and methods allow mechanical control of both tissue composition and spatial organization. The inventive device and methods enable the investigation of dynamic cell-cell interaction in a multitude of applications, such as intercellular communication, spanning embryogenesis, homeostasis, and pathogenic processes.