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18 条记录 (耗时 0.123 秒)

    HOMOGENOUS POPULATIONS OF MOLECULES
    3.
    发明申请
    HOMOGENOUS POPULATIONS OF MOLECULES 审中-公开
    均质的分子群体

    公开(公告)号：US20120184712A1

    公开(公告)日：2012-07-19

    申请号：US13329883

    申请日：2011-12-19

    申请人： Joseph W. Amshey ,  Roumen A. Bogoev

    发明人： Joseph W. Amshey ,  Roumen A. Bogoev

    IPC分类号： C07K14/00

    CPC分类号： G01N33/6842 ,  C40B40/10 ,  G01N2496/00

    摘要： The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.

    摘要翻译： 本发明提供了为一种或多种分子特征制备或被处理成均匀的分子群体。 在一方面，本发明涉及可用于测定未表征的分子（例如蛋白质和核酸）以及其它应用的分子量或表观分子量的分子量标准品。 在一个方面，分子量标准物被预染色。

    Homogenous populations of molecules
    5.
    发明授权
    Homogenous populations of molecules 有权
    均质群体的分子

    公开(公告)号：US09523692B2

    公开(公告)日：2016-12-20

    申请号：US13329883

    申请日：2011-12-19

    申请人： Joseph W. Amshey ,  Roumen A. Bogoev

    发明人： Joseph W. Amshey ,  Roumen A. Bogoev

    IPC分类号： G01N33/68 ,  C40B40/10

    CPC分类号： G01N33/6842 ,  C40B40/10 ,  G01N2496/00

    摘要： The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.

    摘要翻译： 本发明提供了为一种或多种分子特征制备或被处理成均匀的分子群体。 在一方面，本发明涉及可用于测定未表征的分子（例如蛋白质和核酸）以及其它应用的分子量或表观分子量的分子量标准品。 在一个方面，分子量标准物被预染色。

    Three Frame cDNA Expression Libraries for Functional Screening of Proteins
    6.
    发明申请
    Three Frame cDNA Expression Libraries for Functional Screening of Proteins 审中-公开
    用于功能筛选蛋白质的三帧cDNA表达文库

    公开(公告)号：US20100105576A1

    公开(公告)日：2010-04-29

    申请号：US12552986

    申请日：2009-09-02

    申请人： Thomas R. Jackson ,  Adam S. Henry ,  Joseph W. Amshey ,  Roumen A. Bogoev

    发明人： Thomas R. Jackson ,  Adam S. Henry ,  Joseph W. Amshey ,  Roumen A. Bogoev

    IPC分类号： C40B50/06 ,  C40B50/00 ,  C07H21/00

    CPC分类号： C12N15/1093 ,  C12N15/1086

    摘要： Compositions and methods are provided for generating three frame cDNA expression libraries for functional screening of proteins. The invention includes sets of 5′ adapters for cloning cDNA molecules in which the sets include three adapters that can be used to clone a particular cDNA in all three reading frames. The libraries so generated have greater complexity of expressed open reading frames, and thus can improve the success of functional protein screens, such as two hybrid screens. The adapters also have recombination sites for efficient cloning and transfer between systems.

    摘要翻译： 提供组合物和方法用于产生用于蛋白质功能筛选的三个框架cDNA表达文库。 本发明包括用于克隆cDNA分子的5'衔接子的集合，其中所述组包括可用于在所有三个阅读框中克隆特定cDNA的三个衔接子。 如此产生的文库具有更高的表达开放阅读框的复杂性，因此可以提高功能蛋白质屏幕的成功，例如两个混合屏幕。 适配器还具有用于系统之间有效克隆和转移的重组位点。

    METHODS, ARTICLES AND KITS FOR LABELING POLYMER GELS
    8.
    发明申请
    METHODS, ARTICLES AND KITS FOR LABELING POLYMER GELS 审中-公开
    用于标记聚合物凝胶的方法，文章和工具

    公开(公告)号：US20080237043A1

    公开(公告)日：2008-10-02

    申请号：US12039705

    申请日：2008-02-28

    申请人： Joseph W. Amshey ,  Allen Bautista

    发明人： Joseph W. Amshey ,  Allen Bautista

    IPC分类号： C07K1/26

    CPC分类号： G01N27/44704 ,  G01N27/44747

    摘要： The invention provides methods, apparatus and kits for integrally labeling polymeric gels, such as polyacrylamide and agarose gels, with indicia that can serve, inter alia, unambiguously to identify the individual gel, using polymeric film labeled with detectable indicia and coated with materials that bond to the gel matrix as the gel polymerizes.

    摘要翻译： 本发明提供用于整体标记聚合物凝胶（例如聚丙烯酰胺和琼脂糖凝胶）的方法，装置和试剂盒，其标记尤其可以明确地用于鉴别个体凝胶，使用标记有可检测标记的聚合物膜并涂覆有粘合的材料 凝胶基团凝胶聚合。

    Device and methods for subdividing and filtering gel material and extracting molecules therefrom
    9.
    发明授权
    Device and methods for subdividing and filtering gel material and extracting molecules therefrom 失效
    用于细分和过滤凝胶材料并从中提取分子的装置和方法

    公开(公告)号：US06916423B2

    公开(公告)日：2005-07-12

    申请号：US09906792

    申请日：2001-07-18

    申请人： Roumen A. Bogoev ,  Scott E. Whitney ,  Joseph W. Amshey

    发明人： Roumen A. Bogoev ,  Scott E. Whitney ,  Joseph W. Amshey

    IPC分类号： G01N33/48 ,  C07K1/34 ,  C12M1/00 ,  C12M1/33 ,  C12N15/09 ,  C12Q1/68 ,  G01N1/10 ,  G01N1/28 ,  B01D11/04 ,  G01N27/26

    CPC分类号： C07K1/34 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

    摘要： An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.

    摘要翻译： 用于切割凝胶的设备和方法包括凝胶切割管和网状材料。 网状材料延伸穿过管的端部。 为了使用切碎装置对凝胶进行细分，将凝胶放置在打孔管中的网状材料上，在离心机中将切细管，网状材料和凝胶纺丝，迫使凝胶通过网状材料，使凝胶细分 变成大致均匀的较小碎片。 网状材料可以以嵌套管的形式固定到管。 嵌套管嵌套在回收容器的开口内。 网状材料可以与嵌套管中的条状多孔膜串联放置。 将嵌套管和回收容器离心通过使凝胶通过网状材料将凝胶材料细分成碎片。 凝胶随后落在膜上，并且可以在膜上处理以提取或以其它方式处理凝胶材料中的分析物。