公开(公告)号：US20110020649A1

公开(公告)日：2011-01-27

申请号：US12934322

申请日：2009-03-13

申请人： Morihito Ikeda ,  Taisei Nishimi ,  Junichi Katada

发明人： Morihito Ikeda ,  Taisei Nishimi ,  Junichi Katada

IPC分类号： C07F7/02 ,  C08G63/91 ,  C07K2/00 ,  C07K14/76 ,  C07H99/00 ,  C07K14/78 ,  C08F120/10 ,  C08F120/06 ,  C08F20/56 ,  C08F226/10 ,  B32B5/16

CPC分类号： C07F7/1804 ,  G01N33/54353 ,  G01N33/54393 ,  Y10T428/2991

摘要： An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

摘要翻译： 一种用于将生理活性物质固定在固相载体上的固定方法，所述方法包括：使固相载体与由下式（I）表示的含酸酐官能团的硅烷偶联剂接触; 并且在0℃至60℃的范围内的温度下，将生理活性物质与酸酐官能团结合，同时保持接触后的固相载体; 提供生理活性物质固定化载体和用于固定的载体。 此外，包括用下述通式（I）表示的含酸酐官能团的硅烷偶联剂处理的多孔材料的载体，固定在多孔材料上的封端剂; 并提供其制造方法。

公开(公告)号：US08404621B2

公开(公告)日：2013-03-26

申请号：US12934322

申请日：2009-03-13

申请人： Morihito Ikeda ,  Taisei Nishimi ,  Junichi Katada

发明人： Morihito Ikeda ,  Taisei Nishimi ,  Junichi Katada

IPC分类号： C40B60/00

CPC分类号： C07F7/1804 ,  G01N33/54353 ,  G01N33/54393 ,  Y10T428/2991

摘要： An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

摘要翻译： 一种用于将生理活性物质固定在固相载体上的固定方法，所述方法包括：使固相载体与由下式（I）表示的含酸酐官能团的硅烷偶联剂接触; 并且在0℃至60℃的范围内的温度下，将生理活性物质与酸酐官能团结合，同时保持接触后的固相载体; 提供生理活性物质固定化载体和用于固定的载体。 此外，包括用下述通式（I）表示的含酸酐官能团的硅烷偶联剂处理的多孔材料的载体，固定在多孔材料上的封端剂; 并提供其制造方法。

公开(公告)号：US20070122308A1

公开(公告)日：2007-05-31

申请号：US11604698

申请日：2006-11-28

申请人： Morihito Ikeda ,  Taisei Nishimi ,  Hirohiko Tsuzuki ,  Toshihide Ezoe

发明人： Morihito Ikeda ,  Taisei Nishimi ,  Hirohiko Tsuzuki ,  Toshihide Ezoe

IPC分类号： G01N31/22

CPC分类号： G01N21/553 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54373 ,  G01N33/544 ,  G01N2610/00

摘要： An object of the present invention is to provide a biosensor having high capability for suppressing nonspecific protein adsorption and high capability for extracting a target protein. The present invention provides a biosensor which comprises a flow channel that is formed on a substrate and is composed of a detection plane for detecting interaction between a physiologically active substance and a test substance and a non-detection plane where said interaction is not detected, wherein the substrate is a metal surface or a metal film and the surfaces of the detection plane and the non-detection plane are modified with a self-assembled monolayer (SAM) having a hydroxy group and a functional group for binding with a physiologically active substance.

摘要翻译： 本发明的目的是提供一种能够抑制非特异性蛋白质吸附的能力高，提取目标蛋白质的能力高的生物传感器。 本发明提供一种生物传感器，其包括形成在基板上的流路，其由用于检测生理活性物质与检测物质之间的相互作用的检测面以及未检测到相互作用的非检测面构成， 基板是金属表面或金属膜，检测面和非检测面的表面用具有羟基和官能团的自组装单层（SAM）修饰，用于与生理活性物质结合。

公开(公告)号：US08367213B2

公开(公告)日：2013-02-05

申请号：US12442978

申请日：2007-09-28

申请人： Taisei Nishimi ,  Satoshi Hoshi ,  Morihito Ikeda ,  Takashi Kubo ,  Satoshi Tanaka

发明人： Taisei Nishimi ,  Satoshi Hoshi ,  Morihito Ikeda ,  Takashi Kubo ,  Satoshi Tanaka

IPC分类号： B32B9/04

CPC分类号： G01N33/54393 ,  B01L3/50 ,  B01L2300/165 ,  C08J7/047 ,  C08J2483/00 ,  Y10T428/31504 ,  Y10T428/31663 ,  Y10T428/31667 ,  Y10T428/31725 ,  Y10T428/31855 ,  Y10T428/31971

摘要： An object of the present invention is to provide a biochemical instrument having a surface that can simultaneously inhibit adsorption of a biopolymer and that of a hydrophobic low-molecular-weight compound. The present invention provides a biochemical instrument comprising a region that inhibits adsorption of a hydrophobic low-molecular-weight compound and a region that inhibits adsorption of a biopolymer on the surface of a water-insoluble material.

摘要翻译： 本发明的目的是提供一种具有能够同时抑制生物聚合物和疏水性低分子量化合物的吸附的表面的生物化学仪器。 本发明提供了一种生物化学仪器，其包括抑制疏水性低分子量化合物的吸附的区域和抑制生物聚合物吸附在水不溶性材料表面上的区域。

公开(公告)号：US20100035071A1

公开(公告)日：2010-02-11

申请号：US12442978

申请日：2007-09-28

申请人： Taisei Nishimi ,  Satoshi Hoshi ,  Morihito Ikeda ,  Takashi Kubo ,  Satoshi Tanaka

发明人： Taisei Nishimi ,  Satoshi Hoshi ,  Morihito Ikeda ,  Takashi Kubo ,  Satoshi Tanaka

IPC分类号： B32B9/04 ,  B32B27/28 ,  B32B27/34 ,  B32B27/30 ,  B32B27/00 ,  B05D3/00

CPC分类号： G01N33/54393 ,  B01L3/50 ,  B01L2300/165 ,  C08J7/047 ,  C08J2483/00 ,  Y10T428/31504 ,  Y10T428/31663 ,  Y10T428/31667 ,  Y10T428/31725 ,  Y10T428/31855 ,  Y10T428/31971

摘要： An object of the present invention is to provide a biochemical instrument having a surface that can simultaneously inhibit adsorption of a biopolymer and that of a hydrophobic low-molecular-weight compound. The present invention provides a biochemical instrument comprising a region that inhibits adsorption of a hydrophobic low-molecular-weight compound and a region that inhibits adsorption of a biopolymer on the surface of a water-insoluble material.

摘要翻译： 本发明的目的是提供一种具有能够同时抑制生物聚合物和疏水性低分子量化合物的吸附的表面的生物化学仪器。 本发明提供了一种生物化学仪器，其包括抑制疏水性低分子量化合物的吸附的区域和抑制生物聚合物吸附在水不溶性材料表面上的区域。

公开(公告)号：US08585972B2

公开(公告)日：2013-11-19

申请号：US12778555

申请日：2010-05-12

申请人： Taisei Nishimi ,  Toshihide Ezoe ,  Toshiaki Kubo ,  Hidetoshi Tomita

发明人： Taisei Nishimi ,  Toshihide Ezoe ,  Toshiaki Kubo ,  Hidetoshi Tomita

IPC分类号： G01N33/53

CPC分类号： G01N33/54353 ,  G01N33/54373

摘要： One object of the present invention is to provide a biosensor and a production method therefor, by which hydrogel that enables immobilization of a physiologically active substance can be conveniently produced using safe raw materials. The present invention provides a biosensor which comprises a substrate having a metal layer on its surface, wherein a hydrophilic polymer having a reactive functional group capable of reacting with a hydroxyl group or an amino group of a physiologically active substance is bound to the metal layer directly or indirectly via an intermediate layer.

摘要翻译： 本发明的一个目的是提供一种生物传感器及其生产方法，通过该生物传感器及其生产方法，可以使用安全的原料方便地生产能够固定生理活性物质的水凝胶。 本发明提供一种生物传感器，其包括在其表面具有金属层的基材，其中具有能够与生理活性物质的羟基或氨基反应的反应性官能团的亲水性聚合物直接结合到金属层 或间接经由中间层。

公开(公告)号：US07928191B2

公开(公告)日：2011-04-19

申请号：US12201594

申请日：2008-08-29

申请人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

发明人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

IPC分类号： C07K1/16

CPC分类号： G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

摘要： A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

摘要翻译： 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质由10mmol / L至1mol / L的咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

公开(公告)号：US20080188010A1

公开(公告)日：2008-08-07

申请号：US12022606

申请日：2008-01-30

申请人： Yukou Saitoh ,  Toshihide Ezoe ,  Taisei Nishimi

发明人： Yukou Saitoh ,  Toshihide Ezoe ,  Taisei Nishimi

IPC分类号： G01N33/551 ,  B01J19/00

CPC分类号： G01N33/54373

摘要： It is an object of the present invention to provide a biosensor substrate that prevents non-specific adsorption and is used for immobilizing a physiologically active substance. The present invention provides a biosensor substrate which comprises an inorganic oxide film and a hydrophilic polymer having a physiologically active substance-immobilizing group on the substrate, wherein the hydrophilic polymer is bound to the inorganic oxide film through a low molecular compound.

摘要翻译： 本发明的目的是提供一种防止非特异性吸附并用于固定生理活性物质的生物传感器基板。 本发明提供一种生物传感器基板，其包含无机氧化物膜和在基板上具有生理活性物质固定基团的亲水性聚合物，其中亲水性聚合物通过低分子化合物与无机氧化物膜结合。

公开(公告)号：US20060246512A1

公开(公告)日：2006-11-02

申请号：US11377554

申请日：2006-03-17

申请人： Toshihide Ezoe ,  Taisei Nishimi ,  Yukou Saito

发明人： Toshihide Ezoe ,  Taisei Nishimi ,  Yukou Saito

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： C12Q1/002 ,  G01N21/553 ,  G01N21/554 ,  G01N33/5438 ,  Y10S436/805

摘要： It is an object of the present invention to provide a biosensor, wherein non-specific adsorption on a surface that does not retain a physiologically active substance (reference unit) is suppressed. The present invention provides a biosensor which comprises a substrate having at least two types of surfaces on a single plane, wherein at least one of said surfaces does not retain a physiologically active substance, and wherein at least two types of hydrophilic compounds with different molecular weights are bound to said surface that does not retain a physiologically active substance.

摘要翻译： 本发明的目的是提供一种生物传感器，其中抑制了不保留生理活性物质（参考单元）的表面上的非特异性吸附。 本发明提供了一种生物传感器，其包括在单个平面上具有至少两种类型的表面的基底，其中所述表面中的至少一个不保留生理活性物质，并且其中至少两种具有不同分子量的亲水性化合物 结合到不保留生理活性物质的所述表面。

公开(公告)号：US20060040410A1

公开(公告)日：2006-02-23

申请号：US11205183

申请日：2005-08-17

申请人： Taisei Nishimi ,  Toshihide Ezoe ,  Toshiaki Kubo

发明人： Taisei Nishimi ,  Toshihide Ezoe ,  Toshiaki Kubo

IPC分类号： C12M1/34 ,  G01N33/553

CPC分类号： G01N33/54393 ,  G01N33/54373

摘要： It is an object of the present invention to provide a technique of converting carboxylic acid into an active ester with no generation of air bubbles and a technique of stabilizing the obtained active ester. The present invention provides a biosensor wherein a carboxyl group existing on the surface of a substrate thereof is activated with any one compound selected from an uronium salt, a phosphonium salt or a triazine derivative which are defined in the present application, so as to form a carboxylic acid amide group.

摘要翻译： 本发明的目的是提供一种不产生气泡而将羧酸转化成活性酯的技术，以及稳定所得活性酯的技术。 本发明提供一种生物传感器，其中存在于其底物表面上的羧基由选自本申请中定义的铀盐，鏻盐或三嗪衍生物的任何一种化合物活化，从而形成 羧酸酰胺基。