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公开(公告)号：US20110223596A1
公开(公告)日：2011-09-15
申请号：US13030622
申请日：2011-02-18
申请人： Timothy M. WOUDENBERG , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
发明人： Timothy M. WOUDENBERG , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2565/1025 , C12Q2563/185 , C12Q2535/131 , C12Q2563/107
摘要： The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.
摘要翻译： 本发明一般涉及检测分析物，特别是样品中的生物分子。 本发明还涉及通常以多重检测形式检测分析物的存在的组合物，方法和试剂盒。 本发明还涉及用于确定样品中至少一种分析物的存在的方法，采用单分子检测技术单独检测至少一种分子复合物或至少部分分子复合物的方法。
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公开(公告)号：US20090280497A1
公开(公告)日：2009-11-12
申请号：US12493063
申请日：2009-06-26
申请人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
发明人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2565/1025 , C12Q2563/185 , C12Q2535/131 , C12Q2563/107
摘要： The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.
摘要翻译： 本发明一般涉及检测分析物，特别是样品中的生物分子。 本发明还涉及通常以多重检测形式检测分析物的存在的组合物，方法和试剂盒。 本发明还涉及用于确定样品中至少一种分析物的存在的方法，采用单分子检测技术单独检测至少一种分子复合物或至少部分分子复合物的方法。
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3.发明申请公开(公告)号：US20100112572A1
公开(公告)日：2010-05-06
申请号：US12507016
申请日：2009-07-21
申请人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen , Benjamin Schroeder , Paolo Vatta
发明人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen , Benjamin Schroeder , Paolo Vatta
IPC分类号： C12Q1/68
CPC分类号： G01N33/533 , C12Q1/6816 , G01N33/532 , G01N2035/00158 , C12Q2565/102 , C12Q2563/179
摘要： The teachings herein generally relates to probes comprising fabricated coded molecular tags for detecting analytes. The teachings also relate to compositions, methods, and kits for fabricating coded molecular tags comprising a multiplicity or reporter groups in an ordered pattern.
摘要翻译： 本文的教导通常涉及包含用于检测分析物的制造的编码分子标签的探针。 该教导还涉及用于制造编码分子标签的组合物，方法和试剂盒，其包含有序图案中的多个或报告基团。
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公开(公告)号：US07198900B2
公开(公告)日：2007-04-03
申请号：US10652430
申请日：2003-08-29
申请人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
发明人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2565/1025 , C12Q2563/185 , C12Q2535/131 , C12Q2563/107
摘要： The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.
摘要翻译： 本发明一般涉及检测分析物，特别是样品中的生物分子。 本发明还涉及通常以多重检测形式检测分析物的存在的组合物，方法和试剂盒。 本发明还涉及用于确定样品中至少一种分析物的存在的方法，采用单分子检测技术单独检测至少一种分子复合物或至少部分分子复合物的方法。
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5.发明申请公开(公告)号：US20100105886A1
公开(公告)日：2010-04-29
申请号：US12507035
申请日：2009-07-21
申请人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell
发明人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell
IPC分类号： C07H21/04
CPC分类号： C12Q1/6816 , C12Q2565/1025 , C12Q2563/107
摘要： The present teachings generally relate to probes and probes sets for detecting analytes. The teachings also relates to compositions, methods, and kits for assembling probes comprising at least one coded molecular tag.
摘要翻译： 本教导通常涉及用于检测分析物的探针和探针组。 该教导还涉及用于组装包含至少一个编码分子标签的探针的组合物，方法和试剂盒。
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公开(公告)号：US20120316083A1
公开(公告)日：2012-12-13
申请号：US13590794
申请日：2012-08-21
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6876 , G01N27/44726 , G01N27/44791 , C12Q2565/631
摘要： The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.
摘要翻译： 本公开内容涉及通过使用编码分子鉴定靶核酸的方法及其通过纳米孔易位的分析。 通常，经编码的分子经受目标多核苷酸依赖性修饰。 通过在通过纳米孔分析之前将经修饰的编码分子与未修饰的编码分子分离，或者当通过纳米孔分析时，通过检测编码分子的信号图谱的变化来检测修饰的编码分子。
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公开(公告)号：US20100291548A1
公开(公告)日：2010-11-18
申请号：US11685189
申请日：2007-03-12
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6876 , G01N27/44726 , G01N27/44791 , C12Q2565/631
摘要： The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.
摘要翻译： 本公开内容涉及通过使用编码分子鉴定靶核酸的方法及其通过纳米孔易位的分析。 通常，经编码的分子经受目标多核苷酸依赖性修饰。 通过在通过纳米孔分析之前将经修饰的编码分子与未修饰的编码分子分离，或者当通过纳米孔分析时，通过检测编码分子的信号图谱的变化来检测修饰的编码分子。
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公开(公告)号：US06821402B1
公开(公告)日：2004-11-23
申请号：US09154178
申请日：1998-09-16
IPC分类号： G01N2726
CPC分类号： G01N27/44726 , Y10T436/10
摘要： The invention relates to methods, compositions, and systems for calibrating a fluorescent polynucleotide separation apparatus. One aspect of the invention is multiple color calibration standards and their use. A multiple color calibration standard is a mixture of at least two polynucleotides of different length, wherein each of the polynucleotides is labeled with a spectrally distinct fluorescent dye. Another aspect of the invention is to produce total emission temporal profiles of multiple color calibration standards for use in calibrating fluorescent polynucleotide separation apparatus. The peaks corresponding to the fluorescently labeled polynucleotides in the total emission temporal profile may be detected using a peak detector that is driven by changes in the slopes of the total emission temporal profile. Calibration of fluorescent polynucleotide separation apparatus, with various embodiments of the methods of the invention, includes the step of identification of the labeled polynucleotides of the multiple color calibration standards. The process of spectral calibration of a fluorescent polynucleotide separation apparatus using a multiple color calibration standard may include the step of the estimating (extracting) of the dyes' reference spectra, using information from the peak detection process performed on the total emission temporal profile. Other aspects of the invention include systems for separating and detecting fluorescently labeled polynucleotides, wherein the system is designed for spectral calibration in accordance with the subject calibration methods employing multiple color calibration standards. Another aspect of the invention is methods and compositions for detecting the flow of electrical current through a separation channel of a fluorescent polynucleotide separation apparatus. These methods and compositions employ monitoring dyes. Monitoring dyes are fluorescent dyes that are spectrally distinct from the dye on the polynucleotide intended to convey genetic information, e.g., fluorescent polynucleotide sequencing reaction products.
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公开(公告)号：US06991712B2
公开(公告)日：2006-01-31
申请号：US09927791
申请日：2001-08-10
CPC分类号： G01N27/44726 , Y10T436/10
摘要： The invention relates to methods, compositions, and systems for calibrating a fluorescent polynucleotide separation apparatus. One aspect of the invention is multiple color calibration standards and their use. A multiple color calibration standard is a mixture of at least two polynucleotides of different length, wherein each of the polynucleotides is labeled with a spectrally distinct fluorescent dye. Another aspect of the invention is to produce total emission temporal profiles of multiple color calibration standards for use in calibrating fluorescent polynucleotide separation apparatus. The peaks corresponding to the fluorescently labeled polynucleotides in the total emission temporal profile may be detected using a peak detector that is driven by changes in the slopes of the total emission temporal profile. Calibration of fluorescent polynucleotide separation apparatus, with various embodiments of the methods of the invention, includes the step of identification of the labeled polynucleotides of the multiple color calibration standards. The process of spectral calibration of a fluorescent polynucleotide separation apparatus using a multiple color calibration standard may include the step of the estimating (extracting) of the dyes' reference spectra, using information from the peak detection process performed on the total emission temporal profile. Other aspects of the invention include systems for separating and detecting fluorescently labeled polynucleotides, wherein the system is designed for spectral calibration in accordance with the subject calibration methods employing multiple color calibration standards. Another aspect of the invention is methods and compositions for detecting the flow of electrical current through a separation channel of a fluorescent polynucleotide separation apparatus. These methods and compositions employ monitoring dyes. Monitoring dyes are fluorescent dyes that are spectrally distinct from the dye on the polynucleotide intended to convey genetic information, e.g., fluorescent polynucleotide sequencing reaction products.
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公开(公告)号：US06964848B2
公开(公告)日：2005-11-15
申请号：US09816150
申请日：2001-03-26
CPC分类号： C12Q1/6823 , C12Q2527/113 , C12Q2537/165 , C12Q2561/109 , C12Q2561/113
摘要： The invention relates to improved methods of detecting and characterizing polynucleotide sequences and variations in polynucleotide sequences. The invention further relates to a device that can be used to detect and characterize polynucleotide sequences and variations in polynucleotide sequences.
摘要翻译： 本发明涉及检测和表征多核苷酸序列和多核苷酸序列变异的改进方法。 本发明还涉及可用于检测和表征多核苷酸序列和多核苷酸序列变异的装置。
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