Polymer coated nanofibrillar structures and methods for cell maintenance and differentiation
    1.
    发明申请
    Polymer coated nanofibrillar structures and methods for cell maintenance and differentiation 审中-公开
    聚合物包被的纳米纤维结构和细胞维持和分化的方法

    公开(公告)号:US20070082393A1

    公开(公告)日:2007-04-12

    申请号:US11489964

    申请日:2006-07-20

    IPC分类号: C12N5/06 C12N5/08 C12M1/12

    摘要: The invention provides cell-adherent polymeric coatings for articles having a nanofibrillar structure. The coatings include a synthetic, non-biodegradable polymer having at least one pendent amine group, wherein the polymer is covalently immobilized on the article via latent reactive groups. The invention also provides methods for the long term culturing of cells using the polymer coated nanofibrillar structures. The polymer coated nanofibrillar structures of the invention have been found to be particularly useful for the growth and differentiation of cells, including neural precursors.

    摘要翻译: 本发明提供了具有纳米纤维结构的制品的细胞粘附聚合物涂层。 涂层包括具有至少一个侧挂胺基团的合成的,不可生物降解的聚合物,其中聚合物通过潜在的反应性基团共价固定在制品上。 本发明还提供了使用聚合物包被的纳米纤维结构长期培养细胞的方法。 已经发现本发明的聚合物包被的纳米纤丝结构对于包括神经前体在内的细胞的生长和分化特别有用。

    Array print buffers
    4.
    发明申请
    Array print buffers 审中-公开
    阵列打印缓冲区

    公开(公告)号:US20050202447A1

    公开(公告)日:2005-09-15

    申请号:US10800043

    申请日:2004-03-11

    摘要: The present invention relates to compositions suitable for spotting compounds onto a substrate, and methods employing these compositions. The composition can include one or more organic anions of formula I: R(X)m(Y)n. In an embodiment of formula I, R is an organic moiety, X is an anionic moiety, and, Y is a neutral hydrophilic moiety. The composition can include one or more neutral hydrophilic polymers. In an embodiment, the neutral hydrophilic polymer can be represented by formula V: In Formula V, the group in parenthesis represents the polymer backbone, A can be absent or is a carbon or a heteroatom, and B is pendant neutral hydrophilic group. The present invention includes arrays, which can be made with or include compositions according to the present invention and can be made by using the method of the present invention.

    摘要翻译: 本发明涉及适用于将化合物污染在基材上的组合物,以及使用这些组合物的方法。 组合物可以包括一种或多种式I的有机阴离子:R(X)m(Y)n n。 在式I的实施方案中,R是有机部分,X是阴离子部分,Y是中性亲水部分。 组合物可以包括一种或多种中性亲水性聚合物。 在一个实施方案中,中性亲水性聚合物可以由式V表示:在式V中,括号中的基团表示聚合物主链,A可以不存在或是不含碳或杂原子,B是中性亲水基团。 本发明包括可以由本发明的组合物制成的阵列,也可以使用本发明的方法制成。

    ARRAY PRINT BUFFERS
    6.
    发明申请
    ARRAY PRINT BUFFERS 审中-公开
    阵列打印缓冲区

    公开(公告)号:US20090176663A1

    公开(公告)日:2009-07-09

    申请号:US12329696

    申请日:2008-12-08

    IPC分类号: C40B40/08 C40B80/00 C40B50/18

    摘要: The present invention relates to compositions suitable for spotting compounds onto a substrate, and methods employing these compositions. The composition can include one or more organic anions of formula I: R(X)m(Y)n. In an embodiment of formula I, R is an organic moiety, X is an anionic moiety, and, Y is a neutral hydrophilic moiety. The composition can include one or more neutral hydrophilic polymers. In an embodiment, the neutral hydrophilic polymer can be represented by formula V: In Formula V, the group in parenthesis represents the polymer backbone, A can be absent or is a carbon or a heteroatom, and B is pendant neutral hydrophilic group. The present invention includes arrays, which can be made with or include compositions according to the present invention and can be made by using the method of the present invention.

    摘要翻译: 本发明涉及适用于将化合物污染在基材上的组合物,以及使用这些组合物的方法。 组合物可以包括一种或多种式I的有机阴离子:R(X)m(Y)n。 在式I的实施方案中,R是有机部分,X是阴离子部分,Y是中性亲水部分。 组合物可以包括一种或多种中性亲水性聚合物。 在一个实施方案中,中性亲水性聚合物可以由式V表示:在式V中,括号中的基团表示聚合物主链,A可以不存在或是不含碳或杂原子,B是中性亲水基团。 本发明包括可以由本发明的组合物制成的阵列,也可以使用本发明的方法制成。