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    Recombinant yeast PDI and process for production thereof
    1.
    发明授权
    Recombinant yeast PDI and process for production thereof 失效
    重组酵母PDI及其制备方法

    公开(公告)号：US06387683B1

    公开(公告)日：2002-05-14

    申请号：US09368588

    申请日：1999-08-05

    申请人： Nobuyoshi Ishii ,  Yasuo Suzuki ,  Kohji Uchida ,  Yushi Matuo ,  Hideo Tanaka

    发明人： Nobuyoshi Ishii ,  Yasuo Suzuki ,  Kohji Uchida ,  Yushi Matuo ,  Hideo Tanaka

    IPC分类号： C12N990

    CPC分类号： C12N9/90 ,  Y10S435/942

    摘要： The present invention provides a process for producing biologically active recombinant yeast protein disulfide isomerase, which includes the steps of: a) deleting, substituting, or adding one or more bases in a region encoding an endoplasmic reticulum retention signal in a gene encoding protein disulfide isomerase of yeast to modify the gene so as not to encode part or all of the endoplasmic reticulum retention signal; b) incorporating the modified gene into an expression vector; c) transforming host cells with the expression vector; and d) culturing the host cells transformed with the expression vector in a culture medium kept at a nearly neutral pH, thereby causing protein disulfide isomerase to be secreted in an active state outside the host cells. According to the process of the invention, recombinant yeast protein disulfide isomerase can be secreted in a large amount in a biologically active state into a culture medium, and can be collected by a simple purification method.

    摘要翻译： 本发明提供生产生物活性重组酵母蛋白二硫键异构酶的方法，其包括以下步骤：a）在编码蛋白质二硫键异构酶的基因的编码内质网保持信号的区域中缺失，取代或添加一个或多个碱基 的酵母修饰基因，以免编码部分或全部内质网保持信号; b）将修饰的基因并入表达载体中; c）用表达载体转化宿主细胞; 和d）在保持接近中性pH的培养基中培养用表达载体转化的宿主细胞，从而使蛋白质二硫键异构酶以宿主细胞外的活性状态分泌。 根据本发明的方法，可以将重组酵母蛋白二硫键异构酶以大量的生物活性状态分泌到培养基中，并且可以通过简单的纯化方法收集。

    Method for producing DNA nested deletions by an in vitro reaction using transposase
    3.
    发明授权
    Method for producing DNA nested deletions by an in vitro reaction using transposase 失效
    通过使用转座酶的体外反应产生DNA嵌套缺失的方法

    公开(公告)号：US06265159B1

    公开(公告)日：2001-07-24

    申请号：US08998712

    申请日：1997-12-29

    申请人： Yoshinobu Sugino ,  Masayuki Morita ,  Yushi Matuo ,  Kohji Uchida

    发明人： Yoshinobu Sugino ,  Masayuki Morita ,  Yushi Matuo ,  Kohji Uchida

    IPC分类号： C12Q168

    CPC分类号： C12N15/00

    摘要： The present invention provides a method for producing nested deletions in vitro in desired DNA, comprising: 1) preparing a vector containing a DNA fragment in which nested deletions are to be generated and the terminal repeat of a transposon; 2) incubating the vector in vitro with transposase and a DNA replication system; 3) obtaining the vector incorporating the DNA fragment with nested deletions as a reaction product; and optionally, 4) transforming a host cell with the reaction product and growing it; and 5) recovering a vector incorporating said DNA fragment with nested deletions from the grown host cell.

    摘要翻译： 本发明提供了一种用于在所需DNA中体外产生嵌套缺失的方法，包括：1）制备含有其中产生嵌套缺失的DNA片段和转座子末端重复的载体; 2）体外培养载体 具有转座酶和DNA复制体系; 3）获得含有嵌合缺失的DNA片段作为反应产物的载体; 和任选地，4）用反应产物转化宿主细胞并使其生长; 和5）从生长的宿主细胞中回收含有嵌套缺失的所述DNA片段的载体。

    DNA encoding the subunit of avian lactate dehydrogenase
    4.
    发明授权
    DNA encoding the subunit of avian lactate dehydrogenase 有权
    编码禽类乳酸脱氢酶亚基的DNA

    公开(公告)号：US6057141A

    公开(公告)日：2000-05-02

    申请号：US128967

    申请日：1998-08-04

    申请人： Kohji Uchida ,  Hirokazu Matsukawa ,  Tuyosi Fujita ,  Yushi Matuo

    发明人： Kohji Uchida ,  Hirokazu Matsukawa ,  Tuyosi Fujita ,  Yushi Matuo

    IPC分类号： C12N9/04 ,  A23J1/00 ,  C12N5/00

    CPC分类号： C12N9/0006 ,  C12Y101/01027 ,  C12Y101/01028

    摘要： The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present invention is obtained by plaque hybridization or the like from a cDNA library derived from avian heart muscle and encodes the type B subunit protein of avian lactate dehydrogenase having an amino acid sequence shown as SEQ ID NO. 2.

    摘要翻译： 本发明提供编码禽类乳酸脱氢酶的B型亚基蛋白的基因。 本发明的编码禽类乳酸脱氢酶B型亚单位蛋白的基因是通过来自禽心肌的cDNA文库的斑块杂交等获得的，并编码具有氨基酸序列的禽类乳酸脱氢酶的B亚基蛋白 如SEQ ID NO。 2。

    Image recording apparatus and application device thereof
    5.
    发明授权
    Image recording apparatus and application device thereof 失效
    图像记录装置及其应用装置

    公开(公告)号：US06201594B1

    公开(公告)日：2001-03-13

    申请号：US09148476

    申请日：1998-09-08

    申请人： Hiroyuki Kohda ,  Astuhiro Doi ,  Kohji Uchida

    发明人： Hiroyuki Kohda ,  Astuhiro Doi ,  Kohji Uchida

    IPC分类号： G03B2752

    CPC分类号： H04N1/00259 ,  G03B27/32 ,  G03C1/49881 ,  G03C8/4093 ,  G03D13/002 ,  H04N1/00249 ,  H04N1/00275 ,  H04N1/032

    摘要： An image recording apparatus which effects digital exposure while aiming for compactness. An exposure unit digitally exposes a photosensitive material successively while moving above a stage. An application unit moves from the rear side of the exposure unit and applies water successively to the photosensitive material. A superposing unit moves from the rear side of the application unit, and successively superposes an image-receiving material on the photosensitive material. Heat development transfer is carried out, and an image is obtained on the image-receiving material.

    摘要翻译： 一种在瞄准紧凑性的同时实现数字曝光的图像记录装置。 曝光单元在舞台上方移动时，依次数字地曝光感光材料。 应用单元从曝光单元的后侧移动并依次向感光材料施加水。 叠加单元从施加单元的后侧移动，并且将感光材料上的图像接收材料相继叠置。 进行热显影转印，并且在图像接收材料上获得图像。

    Image recording apparatus
    6.
    发明授权
    Image recording apparatus 失效
    图像记录装置

    公开(公告)号：US6115106A

    公开(公告)日：2000-09-05

    申请号：US15526

    申请日：1998-01-29

    申请人： Hiroyuki Kohda ,  Kohji Uchida ,  Atsuhiro Doi ,  Yasuhiro Endo

    发明人： Hiroyuki Kohda ,  Kohji Uchida ,  Atsuhiro Doi ,  Yasuhiro Endo

    IPC分类号： G03B27/32 ,  G03B27/00

    CPC分类号： G03B27/32

    摘要： An image recording apparatus, in which a necessary place can be suitably exposed by simple operation and the workability of inspection, maintenance and the like improves, is obtained. In the image recording apparatus, an upper cover is rotatably connected to and supported at a base stand by an intermediate hinge. Accordingly, there are a first stage opened/closed state, in which the upper cover opens and closes around a supporting shaft, and a second stage opened/closed state, in which the upper cover integrally opens and closes along with the intermediate hinge around another supporting shaft. In the second stage opened state, the inner portion of the base stand is exposed by a large amount, the distal end portion of a stage is lifted, and further, the holding of a supply magazine and a take-up magazine is released. As a result, the innermost place within the apparatus is exposed and the workability of inspection and maintenance, such as replacement of the supply magazine or the take-up magazine, improves.

    摘要翻译： 可以获得能够通过简单的操作适当地曝光必要的地方，检查，维护等的可操作性提高的图像记录装置。 在图像记录装置中，上盖通过中间铰链可转动地连接到支架上并被支撑在基架上。 因此，存在第一阶段打开/关闭状态，其中上盖围绕支撑轴打开和关闭，以及第二阶段打开/关闭状态，其中上盖与中间铰链一起打开和关闭，并且围绕另一状态 支撑轴。 在第二阶段打开状态下，基座的内部被大量暴露，台架的前端部被提起，并且进一步释放供料盒和卷取盒的保持。 结果，设备内的最内部被暴露，并且改进了供应盒或卷取盒的更换的检查和维护的可操作性。

    Vacuum deposition device and pretreatment method for vacuum deposition
    9.
    发明申请
    Vacuum deposition device and pretreatment method for vacuum deposition 审中-公开
    真空沉积装置和真空沉积预处理方法

    公开(公告)号：US20050120959A1

    公开(公告)日：2005-06-09

    申请号：US10952829

    申请日：2004-09-30

    申请人： Yuji Isoda ,  Kohji Uchida

    发明人： Yuji Isoda ,  Kohji Uchida

    IPC分类号： G21K4/00 ,  C09K11/08 ,  C09K11/77 ,  C23C14/06 ,  C23C14/24 ,  C23C14/50 ,  C23C16/00

    CPC分类号： C23C14/505 ,  C09K11/7733 ,  C23C14/0694 ,  C23C14/24 ,  G21K4/00 ,  Y02P20/149

    摘要： The vacuum deposition device includes a vacuum deposition chamber, a vacuum evacuator for evacuating an inside of the chamber, an evaporating unit for evaporating one or more film forming materials in the chamber, a holding unit provided above the evaporating unit for holding a substrate for the vacuum deposition, and a preventing unit for preventing evaporated particles of the one or more materials from adhering to the substrate when the vacuum deposition is carried out at a pressure of 0.05 to 10 Pa. The pretreatment method for the vacuum deposition prepares the prevention unit in the chamber and melts the one or more materials by heating in the chamber at the pressure of 0.05 to 10 Pa while preventing the evaporated particles from adhering to the substrate by using the preventing unit.

    摘要翻译： 真空沉积装置包括真空沉积室，用于抽空室内的真空抽气机，用于蒸发腔室中的一种或多种成膜材料的蒸发单元，设置在蒸发单元上方的保持单元的保持单元，用于保持用于 真空沉积以及当在0.05〜10Pa的压力下进行真空蒸镀时，能够防止一种或多种材料的蒸发的粒子附着在基板上的防止单元，进行真空蒸镀的预处理方法， 并且通过在0.05至10Pa的压力下在室中加热来熔化一种或多种材料，同时通过使用防止单元防止蒸发的颗粒粘附到基板。

    Image recording apparatus and application device thereof
    10.
    发明授权
    Image recording apparatus and application device thereof 失效
    图像记录装置及其应用装置

    公开(公告)号：US6163370A

    公开(公告)日：2000-12-19

    申请号：US149016

    申请日：1998-09-08

    申请人： Hiroyuki Kohda ,  Astuhiro Doi ,  Kohji Uchida

    发明人： Hiroyuki Kohda ,  Astuhiro Doi ,  Kohji Uchida

    IPC分类号： G03B27/30 ,  G03B27/32 ,  G03C1/498 ,  G03C8/40 ,  G03D13/00 ,  H04N1/00 ,  H04N1/032

    CPC分类号： H04N1/00259 ,  G03B27/32 ,  G03C1/49881 ,  G03C8/4093 ,  G03D13/002 ,  H04N1/00249 ,  H04N1/00275 ,  H04N1/032

    摘要： An image recording apparatus which effects digital exposure while aiming for compactness. An exposure unit digitally exposes a photosensitive material successively while moving above a stage. An application unit moves from the rear side of the exposure unit and applies water successively to the photosensitive material. A superposing unit moves from the rear side of the application unit, and successively superposes an image-receiving material on the photosensitive material. Heat development transfer is carried out, and an image is obtained on the image-receiving material.

    摘要翻译： 一种在瞄准紧凑性的同时实现数字曝光的图像记录装置。 曝光单元在舞台上方移动时，依次数字地曝光感光材料。 应用单元从曝光单元的后侧移动并依次向感光材料施加水。 叠加单元从施加单元的后侧移动，并且将感光材料上的图像接收材料相继叠置。 进行热显影转印，并且在图像接收材料上获得图像。